##gff-version 3
##sequence-region BBa_K750012 1 2963
BBa_K750012	.	engineered_region	944	2963	.	+	0	ID=BBa_K750007;Name=BBa_K750007
BBa_K750012	.	CDS	2107	2826	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K750007
BBa_K750012	.	CDS	2107	2826	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K750012	.	terminator	2835	2963	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K750007
BBa_K750012	.	terminator	2835	2914	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750012	.	stem_loop	2846	2889	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750012	.	engineered_region	2835	2914	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750012	.	terminator	2923	2963	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750012	.	engineered_region	2923	2963	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750012	.	stop_codon	2956	2956	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750012	.	polyA_site	2950	2963	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750012	.	stem_loop	2930	2949	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750012	.	engineered_region	944	2017	.	+	0	ID=BBa_K750006;Name=BBa_K750006;Parent=BBa_K750007
BBa_K750012	.	terminator	1889	2017	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K750006
BBa_K750012	.	terminator	1889	1968	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750012	.	stem_loop	1900	1943	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750012	.	engineered_region	1889	1968	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750012	.	terminator	1977	2017	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750012	.	engineered_region	1977	2017	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750012	.	stop_codon	2010	2010	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750012	.	polyA_site	2004	2017	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750012	.	stem_loop	1984	2003	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750012	.	CDS	1100	1855	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K750006
BBa_K750012	.	point_mutation	1591	1591	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K750012	.	engineered_region	1100	1855	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K750012	.	engineered_region	1100	1101	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K750012	.	CDS	1100	1849	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K750012	.	point_mutation	1273	1273	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K750012	.	engineered_tag	1856	1880	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K750012	.	promoter	944	1073	.	+	0	ID=BBa_K206000;Name=BBa_K206000;Parent=BBa_K750006
BBa_K750012	.	operator	1004	1021	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K750012	.	promoter	944	1074	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K750012	.	operator	983	1000	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K750012	.	ribosome_entry_site	1082	1093	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K750006
BBa_K750012	.	conserved_region	1086	1089	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K750012	.	ribosome_entry_site	2089	2100	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K750007
BBa_K750012	.	conserved_region	2093	2096	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K750012	.	promoter	2026	2080	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K750007
BBa_K750012	.	promoter	2045	2050	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K750012	.	engineered_region	2026	2080	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K750012	.	start_codon	2078	2078	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K750012	.	promoter	2067	2072	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K750012	.	non_covalent_binding_site	2026	2045	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K750012	.	engineered_region	1	935	.	+	0	ID=BBa_K750005;Name=BBa_K750005
BBa_K750012	.	promoter	1	130	.	+	0	ID=BBa_K206000;Name=BBa_K206000;Parent=BBa_K750005
BBa_K750012	.	operator	61	78	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K750012	.	promoter	1	131	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K750012	.	operator	40	57	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K750012	.	ribosome_entry_site	139	149	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_K750005
BBa_K750012	.	ribosome_entry_site	145	148	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K750012	.	ribosome_entry_site	139	149	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K750012	.	engineered_region	139	149	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K750012	.	terminator	807	935	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K750005
BBa_K750012	.	terminator	807	886	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750012	.	stem_loop	818	861	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750012	.	engineered_region	807	886	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750012	.	terminator	895	935	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750012	.	engineered_region	895	935	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750012	.	stop_codon	928	928	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750012	.	polyA_site	922	935	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750012	.	stem_loop	902	921	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750012	.	CDS	156	773	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K750005
BBa_K750012	.	engineered_region	156	773	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K750012	.	engineered_tag	774	798	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K750012	.	tag	735	766	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K750012	.	CDS	156	734	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
>BBa_K750012
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagtcacacaggactactagatg
actataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaag
tgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcaga
atatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctg
aaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctg
taggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgc
tgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttcct
tgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaac
agtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtg
tagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacatt
gattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttt
tatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaaaa
acataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgctt
atctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgat
atttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatag
tagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaa
tgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcgga
atgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaa
ttgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagaga
aaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtact
gtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaa
caggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaag
aaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcact
ggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtg
atgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcac
tactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatg
cccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttg
aaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaatt
ggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatt
agacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcc
ttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcct
tcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata