LOCUS       BBa_K750102             1883 bp    DNA     linear       22-Dec-2024
DEFINITION  PtetcIT:atc sensor(J23119 promoter) with cI system
ACCESSION   BBa_K750102
VERSION     BBa_K750102.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..721
                     /label=BBa_C0040
     stem_loop       766..809
                     /label=stem_loop
     misc_feature    755..834
                     /label=BBa_B0010
     terminator      755..834
                     /label=BBa_B0010
     stem_loop       850..869
                     /label=T7 TE
     misc_feature    876..876
                     /label=stop
     polyA_site      870..883
                     /label=polya
     misc_feature    843..883
                     /label=BBa_B0012
     terminator      843..883
                     /label=BBa_B0012
     misc_feature    892..910
                     /label=TetR 1
     misc_feature    892..945
                     /label=BBa_R0040
     promoter        911..916
                     /label=-35
     misc_feature    917..935
                     /label=TetR 2
     promoter        934..939
                     /label=-10
     promoter        892..945
                     /label=BBa_R0040
     misc_feature    44..945
                     /label=BBa_Q04400
     misc_feature    958..961
                     /label=conserved
     RBS             954..965
                     /label=BBa_B0034
     CDS             975..1682
                     /label=cI lambda
     misc_feature    1683..1715
                     /label=LVA
     misc_feature    1722..1746
                     /label=Help:Barcodes
     CDS             972..1746
                     /label=BBa_C0051
     stem_loop       1766..1809
                     /label=stem_loop
     misc_feature    1755..1834
                     /label=BBa_B0010
     terminator      1755..1834
                     /label=BBa_B0010
     stem_loop       1850..1869
                     /label=T7 TE
     misc_feature    1876..1876
                     /label=stop
     polyA_site      1870..1883
                     /label=polya
     misc_feature    1843..1883
                     /label=BBa_B0012
     terminator      1843..1883
                     /label=BBa_B0012
     terminator      1755..1883
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca
      901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg
      961 agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac
     1021 gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg
     1081 tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg
     1141 cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat
     1201 ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt
     1261 cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac
     1321 ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag
     1381 ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct
     1441 ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc
     1501 caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca
     1561 gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca
     1621 atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg
     1681 gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag tgctagtgta
     1741 gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1801 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1861 gggtgggcct ttctgcgttt ata
//