LOCUS       BBa_K750108             2031 bp    DNA     linear       22-Dec-2024
DEFINITION  PcIGLT-pBADGLT:GFP(lva) expression system controlled by cI protein 
            or arabinose
ACCESSION   BBa_K750108
VERSION     BBa_K750108.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     misc_feature    76..78
                     /label=start
     misc_feature    829..831
                     /label=stop
     misc_feature    832..834
                     /label=stop
     misc_feature    792..828
                     /label=LVA tag
     CDS             79..791
                     /label=cds
     misc_feature    76..834
                     /label=BBa_K145015
     stem_loop       854..897
                     /label=stem_loop
     misc_feature    843..922
                     /label=BBa_B0010
     terminator      843..922
                     /label=BBa_B0010
     stem_loop       938..957
                     /label=T7 TE
     misc_feature    964..964
                     /label=stop
     polyA_site      958..971
                     /label=polya
     misc_feature    931..971
                     /label=BBa_B0012
     terminator      931..971
                     /label=BBa_B0012
     terminator      843..971
                     /label=BBa_B0015
     misc_feature    1..971
                     /label=BBa_K750103
     promoter        980..1110
                     /label=promoter
     misc_feature    1019..1036
                     /label=AraI1
     misc_feature    1040..1057
                     /label=AraI2
     promoter        980..1109
                     /label=BBa_K206000
     misc_feature    1122..1125
                     /label=conserved
     RBS             1118..1129
                     /label=BBa_B0034
     misc_feature    1136..1138
                     /label=start
     misc_feature    1889..1891
                     /label=stop
     misc_feature    1892..1894
                     /label=stop
     misc_feature    1852..1888
                     /label=LVA tag
     CDS             1139..1851
                     /label=cds
     misc_feature    1136..1894
                     /label=BBa_K145015
     stem_loop       1914..1957
                     /label=stem_loop
     misc_feature    1903..1982
                     /label=BBa_B0010
     terminator      1903..1982
                     /label=BBa_B0010
     stem_loop       1998..2017
                     /label=T7 TE
     misc_feature    2024..2024
                     /label=stop
     polyA_site      2018..2031
                     /label=polya
     misc_feature    1991..2031
                     /label=BBa_B0012
     terminator      1991..2031
                     /label=BBa_B0012
     terminator      1903..2031
                     /label=BBa_B0015
     misc_feature    980..2031
                     /label=BBa_K750000
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
      121 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      181 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
      241 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
      301 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      361 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
      421 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      481 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
      541 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
      601 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
      661 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
      721 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
      781 ctatacaaaa ggcctgctgc aaacgacgaa aactacgctt tagtagctta ataatactag
      841 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      901 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
      961 ctgcgtttat atactagaga cattgattat ttgcacggcg tcacactttg ctatgccata
     1021 gcaagatagt ccataagatt agcggatcct acctgacgct ttttatcgca actctctact
     1081 gtttctccat accgtttttt tgggctagct actagagaaa gaggagaaat actagatgcg
     1141 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
     1201 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
     1261 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
     1321 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga
     1381 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
     1441 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     1501 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
     1561 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     1621 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
     1681 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     1741 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     1801 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaaa ggcctgctgc
     1861 aaacgacgaa aactacgctt tagtagctta ataatactag agccaggcat caaataaaac
     1921 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1981 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//