LOCUS BBa_K750109 2969 bp DNA linear 25-Nov-2024 DEFINITION cI->GFP(lva)<-arabinose ACCESSION BBa_K750109 VERSION BBa_K750109.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 22..729 /label=cI lambda misc_feature 730..762 /label=LVA misc_feature 769..793 /label=Help:Barcodes CDS 19..793 /label=BBa_C0051 stem_loop 813..856 /label=stem_loop misc_feature 802..881 /label=BBa_B0010 terminator 802..881 /label=BBa_B0010 stem_loop 897..916 /label=T7 TE misc_feature 923..923 /label=stop polyA_site 917..930 /label=polya misc_feature 890..930 /label=BBa_B0012 terminator 890..930 /label=BBa_B0012 terminator 802..930 /label=BBa_B0015 stem_loop 1792..1835 /label=stem_loop misc_feature 1781..1860 /label=BBa_B0010 terminator 1781..1860 /label=BBa_B0010 stem_loop 1876..1895 /label=T7 TE misc_feature 1902..1902 /label=stop polyA_site 1896..1909 /label=polya misc_feature 1869..1909 /label=BBa_B0012 terminator 1869..1909 /label=BBa_B0012 terminator 1781..1909 /label=BBa_B0015 stem_loop 2852..2895 /label=stem_loop misc_feature 2841..2920 /label=BBa_B0010 terminator 2841..2920 /label=BBa_B0010 stem_loop 2936..2955 /label=T7 TE misc_feature 2962..2962 /label=stop polyA_site 2956..2969 /label=polya misc_feature 2929..2969 /label=BBa_B0012 terminator 2929..2969 /label=BBa_B0012 terminator 2841..2969 /label=BBa_B0015 promoter 976..981 /label=-10 promoter 953..958 /label=-35 misc_feature 963..979 /label=OR1 misc_feature 939..955 /label=OR2 misc_feature 939..987 /label=BBa_R0051 promoter 939..987 /label=BBa_R0051 misc_feature 1000..1003 /label=conserved RBS 996..1007 /label=BBa_B0034 misc_feature 1014..1016 /label=start misc_feature 1767..1769 /label=stop misc_feature 1770..1772 /label=stop misc_feature 1730..1766 /label=LVA tag CDS 1017..1729 /label=cds misc_feature 1014..1772 /label=BBa_K145015 stem_loop 1792..1835 /label=stem_loop misc_feature 1781..1860 /label=BBa_B0010 terminator 1781..1860 /label=BBa_B0010 stem_loop 1876..1895 /label=T7 TE misc_feature 1902..1902 /label=stop polyA_site 1896..1909 /label=polya misc_feature 1869..1909 /label=BBa_B0012 terminator 1869..1909 /label=BBa_B0012 terminator 1781..1909 /label=BBa_B0015 misc_feature 939..1909 /label=BBa_K750103 promoter 1918..2048 /label=promoter misc_feature 1957..1974 /label=AraI1 misc_feature 1978..1995 /label=AraI2 promoter 1918..2047 /label=BBa_K206000 misc_feature 2060..2063 /label=conserved RBS 2056..2067 /label=BBa_B0034 misc_feature 2074..2076 /label=start misc_feature 2827..2829 /label=stop misc_feature 2830..2832 /label=stop misc_feature 2790..2826 /label=LVA tag CDS 2077..2789 /label=cds misc_feature 2074..2832 /label=BBa_K145015 stem_loop 2852..2895 /label=stem_loop misc_feature 2841..2920 /label=BBa_B0010 terminator 2841..2920 /label=BBa_B0010 stem_loop 2936..2955 /label=T7 TE misc_feature 2962..2962 /label=stop polyA_site 2956..2969 /label=polya misc_feature 2929..2969 /label=BBa_B0012 terminator 2929..2969 /label=BBa_B0012 terminator 2841..2969 /label=BBa_B0015 misc_feature 1918..2969 /label=BBa_K750000 misc_feature 939..2969 /label=BBa_K750108 ORIGIN 1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag 61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag 121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc 181 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt 241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg 301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg 361 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc 421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca 481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct 541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa 601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc 661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag 721 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc 781 tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 901 caccttcggg tgggcctttc tgcgtttata tactagagta acaccgtgcg tgttgactat 961 tttacctctg gcggtgataa tggttgctac tagagaaaga ggagaaatac tagatgcgta 1021 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta 1081 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta 1141 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta 1201 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact 1261 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg 1321 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa 1381 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat 1441 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag 1501 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc 1561 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca 1621 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt 1681 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaaagg cctgctgcaa 1741 acgacgaaaa ctacgcttta gtagcttaat aatactagag ccaggcatca aataaaacga 1801 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 1861 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagaca 1921 ttgattattt gcacggcgtc acactttgct atgccatagc aagatagtcc ataagattag 1981 cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac cgtttttttg 2041 ggctagctac tagagaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact 2101 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc 2161 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact 2221 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa 2281 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc 2341 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt 2401 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat 2461 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat 2521 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac 2581 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc 2641 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa 2701 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt 2761 acacatggca tggatgaact atacaaaagg cctgctgcaa acgacgaaaa ctacgcttta 2821 gtagcttaat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 2881 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 2941 accttcgggt gggcctttct gcgtttata //