LOCUS BBa_K750110 2847 bp DNA linear 12-May-2024 DEFINITION GFP(lva) expression system controlled by cI and atc(RBS strength:0.3) ACCESSION BBa_K750110 VERSION BBa_K750110.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 misc_feature 62..65 /label=conserved RBS 58..69 /label=BBa_B0034 misc_feature 76..78 /label=start misc_feature 829..831 /label=stop misc_feature 832..834 /label=stop misc_feature 792..828 /label=LVA tag CDS 79..791 /label=cds misc_feature 76..834 /label=BBa_K145015 stem_loop 854..897 /label=stem_loop misc_feature 843..922 /label=BBa_B0010 terminator 843..922 /label=BBa_B0010 stem_loop 938..957 /label=T7 TE misc_feature 964..964 /label=stop polyA_site 958..971 /label=polya misc_feature 931..971 /label=BBa_B0012 terminator 931..971 /label=BBa_B0012 terminator 843..971 /label=BBa_B0015 misc_feature 1..971 /label=BBa_K750103 CDS 1044..1660 /label=tetR misc_feature 1661..1694 /label=SsrA misc_feature 1701..1725 /label=Help:Barcodes CDS 1041..1700 /label=BBa_C0040 promoter 980..1014 /label=BBa_J23116 misc_feature 1027..1030 /label=conserved RBS 1023..1034 /label=BBa_B0034 CDS 1044..1660 /label=tetR misc_feature 1661..1694 /label=SsrA misc_feature 1701..1725 /label=Help:Barcodes CDS 1041..1725 /label=BBa_C0040 stem_loop 1745..1788 /label=stem_loop misc_feature 1734..1813 /label=BBa_B0010 terminator 1734..1813 /label=BBa_B0010 stem_loop 1829..1848 /label=T7 TE misc_feature 1855..1855 /label=stop polyA_site 1849..1862 /label=polya misc_feature 1822..1862 /label=BBa_B0012 terminator 1822..1862 /label=BBa_B0012 terminator 1734..1862 /label=BBa_B0015 misc_feature 980..1862 /label=BBa_K145201 misc_feature 1871..1889 /label=TetR 1 misc_feature 1871..1924 /label=BBa_R0040 promoter 1890..1895 /label=-35 misc_feature 1896..1914 /label=TetR 2 promoter 1913..1918 /label=-10 promoter 1871..1924 /label=BBa_R0040 RBS 1933..1945 /label=RBS-3\Weak RBS 1939..1942 /label=RBS misc_feature 1933..1945 /label=BBa_B0032 RBS 1933..1945 /label=BBa_B0032 misc_feature 1952..1954 /label=start misc_feature 2705..2707 /label=stop misc_feature 2708..2710 /label=stop misc_feature 2668..2704 /label=LVA tag CDS 1955..2667 /label=cds misc_feature 1952..2710 /label=BBa_K145015 stem_loop 2730..2773 /label=stem_loop misc_feature 2719..2798 /label=BBa_B0010 terminator 2719..2798 /label=BBa_B0010 stem_loop 2814..2833 /label=T7 TE misc_feature 2840..2840 /label=stop polyA_site 2834..2847 /label=polya misc_feature 2807..2847 /label=BBa_B0012 terminator 2807..2847 /label=BBa_B0012 misc_feature 1871..2847 /label=BBa_K145205 misc_feature 980..2847 /label=BBa_K145280 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa 61 gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt 121 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt 181 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt 241 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca 301 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa 361 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa 421 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac 481 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac 541 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc 601 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta 661 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga 721 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa 781 ctatacaaaa ggcctgctgc aaacgacgaa aactacgctt tagtagctta ataatactag 841 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 901 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 961 ctgcgtttat atactagagt tgacagctag ctcagtccta gggactatgc tagctactag 1021 agaaagagga gaaatactag atgtccagat tagataaaag taaagtgatt aacagcgcat 1081 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc 1141 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg 1201 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa 1261 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc 1321 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg 1381 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac 1441 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg 1501 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta 1561 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca 1621 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact 1681 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagccaggca 1741 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 1801 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 1861 tatactagag tccctatcag tgatagagat tgacatccct atcagtgata gagatactga 1921 gcactactag agtcacacag gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg 1981 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag 2041 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac 2101 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg 2161 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga 2221 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc 2281 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt 2341 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt 2401 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa 2461 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga 2521 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga 2581 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac 2641 acatggcatg gatgaactat acaaaaggcc tgctgcaaac gacgaaaact acgctttagt 2701 agcttaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 2761 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 2821 cttcgggtgg gcctttctgc gtttata //