LOCUS       BBa_K750111             1867 bp    DNA     linear       22-Dec-2024
DEFINITION  PtetGLT:GFP(lva) expression system controlled by 
            (anhydro)tetracyclin
ACCESSION   BBa_K750111
VERSION     BBa_K750111.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..746
                     /label=BBa_C0040
     stem_loop       766..809
                     /label=stem_loop
     misc_feature    755..834
                     /label=BBa_B0010
     terminator      755..834
                     /label=BBa_B0010
     stem_loop       850..869
                     /label=T7 TE
     misc_feature    876..876
                     /label=stop
     polyA_site      870..883
                     /label=polya
     misc_feature    843..883
                     /label=BBa_B0012
     terminator      843..883
                     /label=BBa_B0012
     terminator      755..883
                     /label=BBa_B0015
     misc_feature    892..910
                     /label=TetR 1
     misc_feature    892..945
                     /label=BBa_R0040
     promoter        911..916
                     /label=-35
     misc_feature    917..935
                     /label=TetR 2
     promoter        934..939
                     /label=-10
     promoter        892..945
                     /label=BBa_R0040
     misc_feature    958..961
                     /label=conserved
     RBS             954..965
                     /label=BBa_B0034
     misc_feature    972..974
                     /label=start
     misc_feature    1725..1727
                     /label=stop
     misc_feature    1728..1730
                     /label=stop
     misc_feature    1688..1724
                     /label=LVA tag
     CDS             975..1687
                     /label=cds
     misc_feature    972..1730
                     /label=BBa_K145015
     stem_loop       1750..1793
                     /label=stem_loop
     misc_feature    1739..1818
                     /label=BBa_B0010
     terminator      1739..1818
                     /label=BBa_B0010
     stem_loop       1834..1853
                     /label=T7 TE
     misc_feature    1860..1860
                     /label=stop
     polyA_site      1854..1867
                     /label=polya
     misc_feature    1827..1867
                     /label=BBa_B0012
     terminator      1827..1867
                     /label=BBa_B0012
     terminator      1739..1867
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca
      901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg
      961 agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
     1021 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
     1081 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
     1141 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc
     1201 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
     1261 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
     1321 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
     1381 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
     1441 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
     1501 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
     1561 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
     1621 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
     1681 acaaaaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa tactagagcc
     1741 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1801 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1861 gtttata
//