LOCUS       BBa_K750113             2047 bp    DNA     linear       22-Dec-2024
DEFINITION  pBADcIT-PcIGLT: Pbad->cI(lva)->PcI->GFP(lva)
ACCESSION   BBa_K750113
VERSION     BBa_K750113.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             160..867
                     /label=cI lambda
     misc_feature    868..900
                     /label=LVA
     misc_feature    907..931
                     /label=Help:Barcodes
     CDS             157..931
                     /label=BBa_C0051
     stem_loop       951..994
                     /label=stem_loop
     misc_feature    940..1019
                     /label=BBa_B0010
     terminator      940..1019
                     /label=BBa_B0010
     stem_loop       1035..1054
                     /label=T7 TE
     misc_feature    1061..1061
                     /label=stop
     polyA_site      1055..1068
                     /label=polya
     misc_feature    1028..1068
                     /label=BBa_B0012
     terminator      1028..1068
                     /label=BBa_B0012
     terminator      940..1068
                     /label=BBa_B0015
     misc_feature    1..1068
                     /label=BBa_K750104
     promoter        1114..1119
                     /label=-10
     promoter        1091..1096
                     /label=-35
     misc_feature    1101..1117
                     /label=OR1
     misc_feature    1077..1093
                     /label=OR2
     misc_feature    1077..1125
                     /label=BBa_R0051
     promoter        1077..1125
                     /label=BBa_R0051
     misc_feature    1138..1141
                     /label=conserved
     RBS             1134..1145
                     /label=BBa_B0034
     misc_feature    1152..1154
                     /label=start
     misc_feature    1905..1907
                     /label=stop
     misc_feature    1908..1910
                     /label=stop
     misc_feature    1868..1904
                     /label=LVA tag
     CDS             1155..1867
                     /label=cds
     misc_feature    1152..1910
                     /label=BBa_K145015
     stem_loop       1930..1973
                     /label=stem_loop
     misc_feature    1919..1998
                     /label=BBa_B0010
     terminator      1919..1998
                     /label=BBa_B0010
     stem_loop       2014..2033
                     /label=T7 TE
     misc_feature    2040..2040
                     /label=stop
     polyA_site      2034..2047
                     /label=polya
     misc_feature    2007..2047
                     /label=BBa_B0012
     terminator      2007..2047
                     /label=BBa_B0012
     terminator      1919..2047
                     /label=BBa_B0015
     misc_feature    1077..2047
                     /label=BBa_K750103
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta
      181 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat
      241 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt
      301 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa
      361 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg
      421 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct
      481 catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag
      541 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt
      601 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc
      661 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat
      721 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac
      781 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct
      841 agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct
      901 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa
      961 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1021 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtaac
     1081 accgtgcgtg ttgactattt tacctctggc ggtgataatg gttgctacta gagaaagagg
     1141 agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
     1201 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
     1261 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
     1321 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc
     1381 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
     1441 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
     1501 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
     1561 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
     1621 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
     1681 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
     1741 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
     1801 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
     1861 acaaaaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa tactagagcc
     1921 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1981 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     2041 gtttata
//