LOCUS BBa_K759002 3306 bp DNA linear 25-Nov-2024 DEFINITION E. coli agregation (Ag43) device induced by T7 promoter. ACCESSION BBa_K759002 VERSION BBa_K759002.1 FEATURES Location/Qualifiers promoter 1..23 /label=BBa_I719005 misc_feature 36..39 /label=conserved RBS 32..43 /label=BBa_B0034 CDS 50..3169 /label=agn43 CDS 50..3169 /label=BBa_K346007 stem_loop 3189..3232 /label=stem_loop misc_feature 3178..3257 /label=BBa_B0010 terminator 3178..3257 /label=BBa_B0010 stem_loop 3273..3292 /label=T7 TE misc_feature 3299..3299 /label=stop polyA_site 3293..3306 /label=polya misc_feature 3266..3306 /label=BBa_B0012 terminator 3266..3306 /label=BBa_B0012 terminator 3178..3306 /label=BBa_B0015 ORIGIN 1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tgaaacgaca 61 tctgaatacc tgctacaggc tggtatggaa tcacatgacg ggcgctttcg tggttgcctc 121 cgaactggcc cgcgcacggg gtaaacgtgg cggtgtggcg gttgcactgt ctcttgccgc 181 agtcacgtca ctcccggtgc tggctgctga catcgttgtg cacccgggag aaaccgtgaa 241 cggcggaaca ctggcaaatc atgacaacca gattgtcttc ggtacgacca acggaatgac 301 catcagtacc gggctggagt atgggccgga taacgaggcc aataccggcg ggcaatgggt 361 acaggatggc ggaacagcca acaaaacgac tgtcaccagt ggtggtcttc agagagtgaa 421 ccccggtgga agtgtctcag acacggttat cagtgccgga ggcggacaga gccttcaggg 481 acgggctgtg aacaccacgc tgaatggtgg cgaacagtgg atgcatgagg gggcgatagc 541 cacaggaacc gtcattaatg ataagggctg gcaggtcgtc aagcccggta cagtggcaac 601 ggataccgtt gttaataccg gggcggaagg gggaccggat gcagaaaacg gtgataccgg 661 gcagtttgtt cgcggggatg ccgtacgcac aaccatcaat aaaaacggtc gccagattgt 721 gagagctgaa ggaacggcaa ataccactgt ggtttatgcc ggcggcgacc agactgtaca 781 tggtcacgca ctggatacca cgctgaatgg gggataccag tatgtgcaca acggcggtac 841 agcgtctgac actgttgtga acagtgacgg ctggcagatt gtcaaaaacg ggggtgtggc 901 cgggaatacc accgttaatc agaagggcag actgcaagtg gacgccggtg gtacagccac 961 gaatgtcacc ctgaagcagg gcggcgcact ggttaccagt acggctgcaa ccgttaccgg 1021 cataaaccgc ctgggagcat tctctgttgt ggagggtaaa gctgataatg tcgtactgga 1081 aaatggcgga cgcctggatg tgctgaccgg acacacagcc actaataccc gcgtggatga 1141 tggcggaacg ctggatgtcc gcaacggtgg caccgccacc accgtatcca tgggaaatgg 1201 cggtgtactg ctggccgatt ccggtgccgc tgtcagtggt acccggagcg acggaaaggc 1261 attcagtatc ggaggcggtc aggcggatgc cctgatgctg gaaaaaggca gttcattcac 1321 gctgaacgcc ggtgatacgg ccacggatac cacggtaaat ggcggactgt tcaccgccag 1381 gggcggcaca ctggcgggca ccaccacgct gaataacggc gccatactta ccctttccgg 1441 gaagacggtg aacaacgata ccctgaccat ccgtgaaggc gatgcactcc tgcaaggagg 1501 ctctctcacc ggtaacggca gcgtggaaaa atcaggaagt ggcacactca ctgtcagcaa 1561 caccacactc acccagaaag ccgtcaacct gaatgaaggc acgctgacgc tgaacgacag 1621 taccgtcacc acggatgtca ttgctcagcg cggtacagcc ctgaagctga ccggcagcac 1681 tgtgctgaac ggtgccattg accccacgaa tgtcactctc gcctccggtg ccacctggaa 1741 tatccccgat aacgccacgg tgcagtcggt ggtggatgac ctcagccatg ccggacagat 1801 tcatttcacc tccacccgca cagggaagtt cgtaccggca accctgaaag tgaaaaacct 1861 gaacggacag aatggcacca tcagcctgcg tgtacgcccg gatatggcac agaacaatgc 1921 tgacagactg gtcattgacg gcggcagggc aaccggaaaa accatcctga acctggtgaa 1981 cgccggcaac agtgcgtcgg ggctggcgac cagcggtaag ggtattcagg tggtggaagc 2041 cattaacggt gccaccacgg aggaaggggc ctttgtccag gggaacaggc tgcaagccgg 2101 tgcctttaac tactccctca accgggacag tgatgagagc tggtatctgc gcagtgaaaa 2161 tgcttatcgt gcagaagtcc ccctgtatgc ctccatgctg acacaggcaa tggactatga 2221 ccggattgtg gcaggctccc gcagccatca gaccggtgta aatggtgaaa acaacagcgt 2281 ccgtctcagc attcagggcg gtcatctcgg tcacgataac aatggcggta ttgcccgtgg 2341 ggccacgccg gaaagcagcg gcagctatgg attcgtccgt ctggagggtg acctgatgag 2401 aacagaggtt gccggtatgt ctgtgaccgc gggggtatat ggtgctgctg gccattcttc 2461 cgttgatgtt aaggatgatg acggctcccg tgccggcacg gtccgggatg atgccggcag 2521 cctgggcgga tacctgaatc tggtacacac gtcctccggc ctgtgggctg acattgtggc 2581 acagggaacc cgccacagca tgaaagcgtc atcggacaat aacgacttcc gcgcccgggg 2641 ctggggctgg ctgggctcac tggaaaccgg tctgcccttc agtatcactg acaacctgat 2701 gctggagcca caactgcaat atacctggca gggactttcc ctggatgacg gtaaggacaa 2761 cgccggttat gtgaagttcg ggcatggcag tgcacaacat gtgcgtgccg gtttccgtct 2821 gggcagccac aacgatatga cctttggcga aggcacctca tcccgtgccc ccctgcgtga 2881 cagtgcaaaa cacagtgtga gtgaattacc ggtgaactgg tgggtacagc cttctgttat 2941 ccgcaccttc agctcccggg gagatatgcg tgtggggact tccactgcaa gcagcgggat 3001 gacgttctct ccctcacaga atggcacatc actggacctg caagccggac tggaagcccg 3061 tgtccgggaa aatatcaccc tgggcgttca ggccggttat gcccacagcg tcagcggcag 3121 cagcgctgaa gggtataacg gtcaggccac actgaatgtg accttctgat actagagcca 3181 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 3241 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 3301 tttata //