LOCUS BBa_K759010 3918 bp DNA linear 26-Nov-2024 DEFINITION P3HB producing construct (without a promoter) ACCESSION BBa_K759010 VERSION BBa_K759010.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 21..1809 /label=BBa_K342001 misc_feature 1822..1825 /label=conserved RBS 1818..1829 /label=BBa_B0034 CDS 1836..3017 /label=BBa_K759004 misc_feature 3030..3033 /label=conserved RBS 3026..3037 /label=BBa_B0034 CDS 3044..3781 /label=BBa_K759005 stem_loop 3801..3844 /label=stem_loop misc_feature 3790..3869 /label=BBa_B0010 terminator 3790..3869 /label=BBa_B0010 stem_loop 3885..3904 /label=T7 TE misc_feature 3911..3911 /label=stop polyA_site 3905..3918 /label=polya misc_feature 3878..3918 /label=BBa_B0012 terminator 3878..3918 /label=BBa_B0012 terminator 3790..3918 /label=BBa_B0015 ORIGIN 1 aaagaggaga aatactagag aaggaggtat ataatggcga ccggcaaagg cgcggcagct 61 tccacgcagg aaggcaagtc ccaaccattc aaggtcacgc cggggccatt cgatccagcc 121 acatggctgg aatggtcccg ccagtggcag ggcactgaag gcaacggcca cgcgaccgcg 181 tccggcattc cgggcctgga tgcgctggca ggcgtcaaga tcgcgccggc gcagctgggt 241 gatatccagc agcgctacat gaaggacttc tcagcgctgt ggcaggccat ggccgagggc 301 aaggccgagg ccaccggtcc gctgcacgac cggcgcttcg ccggcgacgc atggcgcacc 361 aacctcccat atcgcttcgc tgccgcgttc tacctgctca atgcgcgcgc cttgaccgag 421 ctggccgatg ccgtcgaggc cgatgccaag acccgccagc gcatccgctt cgcgatctcg 481 caatgggtcg atgcgatgtc gcccgccaac ttccttgcca ccaatcccga ggcgcagcgc 541 ctgctgatcg agtcgggcgg cgaatcgctg cgtgccggcg tgcgcaacat gatggaagac 601 ctgacacgcg gcaagatctc gcagaccgac gagagcgcgt ttgaggtcgg ccgcaatgtc 661 gcggtgaccg aaggcgccgt ggtcttcgag aacgagtact tccagctgtt gcagtacaag 721 ccgctgaccg acaaggtgca cgcgcgcccg ctgctgatgg tgccgccgtg catcaacaag 781 tactacatcc tggacatgca gccggagagc tcgctggtgc gccatgtggt ggagcaggga 841 catacggtgt ttctggtgtc gtggcgcaat ccggacgcca gcatggccgg cagcacctgg 901 gacgactaca tcgagcacgc ggccatccgc gccatcgaag tcgcgcgcga catcagcggc 961 caggacaaga tcaacgtgct cggcttctgc gtgggcggca ccattgtctc gaccgcgctg 1021 gcggtgctgg ccgcgcgcgg cgagcacccg gccgccagcg tcacgctgct gaccacgctg 1081 ctggactttg ccgacacggg catcctcgac gtctttgtcg acgagggcca tgtgcagttg 1141 cgcgaggcca cgctgggcgg cggcgccggc gcgccgtgcg cgctgctgcg cggccttgag 1201 ctggccaata ccttctcgtt cttgcgcccg aacgacctgg tgtggaacta cgtggtcgac 1261 aactacctga agggcaacac gccggtgccg ttcgacctgc tgttctggaa cggcgacgcc 1321 accaacctgc cggggccgtg gtactgctgg tacctgcgcc acacctacat gcagaacgag 1381 ctcaaggtac cgggcaagct gaccgtgtgc ggcgtgccgg tggacctggc cagcatcgac 1441 gtgccgacct atatctacgg ctcgcgcgaa gaccatatcg tgccgtggac cgcggcctat 1501 gcctcgaccg cgctgctggc gaacaagctg cgcttcgtgc tgggtgcgtc gggccatatc 1561 gccggtgtga tcaacccgcc ggccaagaac aagcgcagcc actggactaa cgatgcgctg 1621 ccggagtcgc cgcagcaatg gctggccggc gccatcgagc atcacggcag ctggtggccg 1681 gactggaccg catggctggc cgggcaggcc ggcgcgaaac gcgccgcgcc cgccaactat 1741 ggcaatgcgc gctatcgcgc aatcgaaccc gcgcctgggc gatacgtcaa agccaaggca 1801 tgataataat actagagaaa gaggagaaat actagatgac tgacgttgtc atcgtatccg 1861 ccgcccgcac cgcggtcggc aagtttggcg gctcgctggc caagatcccg gcaccggaac 1921 tgggtgccgt ggtcatcaag gccgcgctgg agcgcgccgg cgtcaagccg gagcaggtga 1981 gcgaagtcat catgggccag gtgctgaccg ccggttcggg ccagaacccc gcacgccagg 2041 ccgcgatcaa ggccggcctg ccggcgatgg tgccggccat gaccatcaac aaggtgtgcg 2101 gctcgggcct gaaggccgtg atgctggccg ccaacgcgat catggcgggc gacgccgaga 2161 tcgtggtggc cggcggccag gaaaacatga gcgccgcccc gcacgtgctg ccgggctcgc 2221 gcgatggttt ccgcatgggc gatgccaagc tggtcgacac catgatcgtc gacggcctgt 2281 gggacgtgta caaccagtac cacatgggca tcaccgccga gaacgtggcc aaggaatacg 2341 gcatcacacg cgaggcgcag gatgagttcg ccgtcggctc gcagaacaag gccgaagccg 2401 cgcagaaggc cggcaagttt gacgaagaga tcgtcccggt gctgatcccg cagcgcaagg 2461 gcgacccggt ggccttcaag accgacgagt tcgtgcgcca gggcgccacg ctggacagca 2521 tgtccggcct caagcccgcc ttcgacaagg ccggcacggt gaccgcggcc aacgcctcgg 2581 gcctgaacga cggcgccgcc gcggtggtgg tgatgtcggc ggccaaggcc aaggaactgg 2641 gcctgacccc gctggccacg atcaagagct atgccaacgc cggtgtcgat cccaaggtga 2701 tgggcatggg cccggtgccg gcctccaagc gcgccctgtc gcgcgccgag tggaccccgc 2761 aagacctgga cctgatggag atcaacgagg cctttgccgc gcaggcgctg gcggtgcacc 2821 agcagatggg ctgggacacc tccaaggtca atgtgaacgg cggcgccatc gccatcggcc 2881 acccgatcgg cgcgtcgggc tgccgtatcc tggtgacgct gctgcacgag atgaagcgcc 2941 gtgacgcgaa gaagggcctg gcctcgctgt gcatcggcgg cggcatgggc gtggcgctgg 3001 cagtcgagcg caaatactac tagagaaaga ggagaaatac tagatgactc agcgcattgc 3061 gtatgtgacc ggcggcatgg gtggtatcgg aaccgccatt tgccagcggc tggccaagga 3121 tggctttcgt gtggtggccg gttgcggccc caactcgccg cgccgcgaaa agtggctgga 3181 gcagcagaag gccctgggct tcgatttcat tgcctcggaa ggcaatgtgg ctgactggga 3241 ctcgaccaag accgcattcg acaaggtcaa gtccgaggtc ggcgaggttg atgtgctgat 3301 caacaacgcc ggtatcaccc gcgacgtggt gttccgcaag atgacccgcg ccgactggga 3361 tgcggtgatc gacaccaacc tgacctcgct gttcaacgtc accaagcagg tgatcgacgg 3421 catggccgac cgtggctggg gccgcatcgt caacatctcg tcggtgaacg ggcagaaggg 3481 ccagttcggc cagaccaact actccaccgc caaggccggc ctgcatggct tcaccatggc 3541 actggcgcag gaagtggcga ccaagggcgt gaccgtcaac acggtctctc cgggctatat 3601 cgccaccgac atggtcaagg cgatccgcca ggacgtgctc gacaagatcg tcgcgacgat 3661 cccggtcaag cgcctgggcc tgccggaaga gatcgcctcg atctgcgcct ggttgtcgtc 3721 ggaggagtcc ggtttctcga ccggcgccga cttctcgctc aacggcggcc tgcatatggg 3781 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 3841 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 3901 ggcctttctg cgtttata //