LOCUS BBa_K759012 5242 bp DNA linear 25-Nov-2024 DEFINITION eCFP + E. coli aggregation (Ag43) device induced by pBAD promoter. ACCESSION BBa_K759012 VERSION BBa_K759012.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1959 /label=BBa_E0020 misc_feature 1972..1975 /label=conserved RBS 1968..1979 /label=BBa_B0034 CDS 1986..5105 /label=agn43 CDS 1986..5105 /label=BBa_K346007 stem_loop 5125..5168 /label=stem_loop misc_feature 5114..5193 /label=BBa_B0010 terminator 5114..5193 /label=BBa_B0010 stem_loop 5209..5228 /label=T7 TE misc_feature 5235..5235 /label=stop polyA_site 5229..5242 /label=polya misc_feature 5202..5242 /label=BBa_B0012 terminator 5202..5242 /label=BBa_B0012 terminator 5114..5242 /label=BBa_B0015 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg 1261 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg ccacaagttc 1321 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 1381 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc 1441 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 1501 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 1561 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 1621 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc 1681 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc 1741 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 1801 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 1861 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 1921 gggatcactc tcggcatgga cgagctgtac aagtaataat actagagaaa gaggagaaat 1981 actagatgaa acgacatctg aatacctgct acaggctggt atggaatcac atgacgggcg 2041 ctttcgtggt tgcctccgaa ctggcccgcg cacggggtaa acgtggcggt gtggcggttg 2101 cactgtctct tgccgcagtc acgtcactcc cggtgctggc tgctgacatc gttgtgcacc 2161 cgggagaaac cgtgaacggc ggaacactgg caaatcatga caaccagatt gtcttcggta 2221 cgaccaacgg aatgaccatc agtaccgggc tggagtatgg gccggataac gaggccaata 2281 ccggcgggca atgggtacag gatggcggaa cagccaacaa aacgactgtc accagtggtg 2341 gtcttcagag agtgaacccc ggtggaagtg tctcagacac ggttatcagt gccggaggcg 2401 gacagagcct tcagggacgg gctgtgaaca ccacgctgaa tggtggcgaa cagtggatgc 2461 atgagggggc gatagccaca ggaaccgtca ttaatgataa gggctggcag gtcgtcaagc 2521 ccggtacagt ggcaacggat accgttgtta ataccggggc ggaaggggga ccggatgcag 2581 aaaacggtga taccgggcag tttgttcgcg gggatgccgt acgcacaacc atcaataaaa 2641 acggtcgcca gattgtgaga gctgaaggaa cggcaaatac cactgtggtt tatgccggcg 2701 gcgaccagac tgtacatggt cacgcactgg ataccacgct gaatggggga taccagtatg 2761 tgcacaacgg cggtacagcg tctgacactg ttgtgaacag tgacggctgg cagattgtca 2821 aaaacggggg tgtggccggg aataccaccg ttaatcagaa gggcagactg caagtggacg 2881 ccggtggtac agccacgaat gtcaccctga agcagggcgg cgcactggtt accagtacgg 2941 ctgcaaccgt taccggcata aaccgcctgg gagcattctc tgttgtggag ggtaaagctg 3001 ataatgtcgt actggaaaat ggcggacgcc tggatgtgct gaccggacac acagccacta 3061 atacccgcgt ggatgatggc ggaacgctgg atgtccgcaa cggtggcacc gccaccaccg 3121 tatccatggg aaatggcggt gtactgctgg ccgattccgg tgccgctgtc agtggtaccc 3181 ggagcgacgg aaaggcattc agtatcggag gcggtcaggc ggatgccctg atgctggaaa 3241 aaggcagttc attcacgctg aacgccggtg atacggccac ggataccacg gtaaatggcg 3301 gactgttcac cgccaggggc ggcacactgg cgggcaccac cacgctgaat aacggcgcca 3361 tacttaccct ttccgggaag acggtgaaca acgataccct gaccatccgt gaaggcgatg 3421 cactcctgca aggaggctct ctcaccggta acggcagcgt ggaaaaatca ggaagtggca 3481 cactcactgt cagcaacacc acactcaccc agaaagccgt caacctgaat gaaggcacgc 3541 tgacgctgaa cgacagtacc gtcaccacgg atgtcattgc tcagcgcggt acagccctga 3601 agctgaccgg cagcactgtg ctgaacggtg ccattgaccc cacgaatgtc actctcgcct 3661 ccggtgccac ctggaatatc cccgataacg ccacggtgca gtcggtggtg gatgacctca 3721 gccatgccgg acagattcat ttcacctcca cccgcacagg gaagttcgta ccggcaaccc 3781 tgaaagtgaa aaacctgaac ggacagaatg gcaccatcag cctgcgtgta cgcccggata 3841 tggcacagaa caatgctgac agactggtca ttgacggcgg cagggcaacc ggaaaaacca 3901 tcctgaacct ggtgaacgcc ggcaacagtg cgtcggggct ggcgaccagc ggtaagggta 3961 ttcaggtggt ggaagccatt aacggtgcca ccacggagga aggggccttt gtccagggga 4021 acaggctgca agccggtgcc tttaactact ccctcaaccg ggacagtgat gagagctggt 4081 atctgcgcag tgaaaatgct tatcgtgcag aagtccccct gtatgcctcc atgctgacac 4141 aggcaatgga ctatgaccgg attgtggcag gctcccgcag ccatcagacc ggtgtaaatg 4201 gtgaaaacaa cagcgtccgt ctcagcattc agggcggtca tctcggtcac gataacaatg 4261 gcggtattgc ccgtggggcc acgccggaaa gcagcggcag ctatggattc gtccgtctgg 4321 agggtgacct gatgagaaca gaggttgccg gtatgtctgt gaccgcgggg gtatatggtg 4381 ctgctggcca ttcttccgtt gatgttaagg atgatgacgg ctcccgtgcc ggcacggtcc 4441 gggatgatgc cggcagcctg ggcggatacc tgaatctggt acacacgtcc tccggcctgt 4501 gggctgacat tgtggcacag ggaacccgcc acagcatgaa agcgtcatcg gacaataacg 4561 acttccgcgc ccggggctgg ggctggctgg gctcactgga aaccggtctg cccttcagta 4621 tcactgacaa cctgatgctg gagccacaac tgcaatatac ctggcaggga ctttccctgg 4681 atgacggtaa ggacaacgcc ggttatgtga agttcgggca tggcagtgca caacatgtgc 4741 gtgccggttt ccgtctgggc agccacaacg atatgacctt tggcgaaggc acctcatccc 4801 gtgcccccct gcgtgacagt gcaaaacaca gtgtgagtga attaccggtg aactggtggg 4861 tacagccttc tgttatccgc accttcagct cccggggaga tatgcgtgtg gggacttcca 4921 ctgcaagcag cgggatgacg ttctctccct cacagaatgg cacatcactg gacctgcaag 4981 ccggactgga agcccgtgtc cgggaaaata tcaccctggg cgttcaggcc ggttatgccc 5041 acagcgtcag cggcagcagc gctgaagggt ataacggtca ggccacactg aatgtgacct 5101 tctgatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 5161 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 5221 ggtgggcctt tctgcgttta ta //