eCFP + E. coli aggregation (Ag43) device induced by pBAD promoter.
Ag43(BBA_K346007) was derived from E. coli K-12 strain by Peking 2010 iGEM.
In the presence of L-arabinose, pBAD promoter is induced and then wildtype Antigen 43 (Ag43) protein and eCFP (engineered cyan fluorescent protein)is produced. The wildtype Ag43 have 6 PstI sites. eCFP locates in the upstream of Ag43 for estimating the relationship between expressed protein amount and aggregation efficiency.
6 PstI sites exists in Ag43 coding site so we assembled this composite part without using PstI.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
engineered cyan fluorescent protein derived from A. victoria GFP
Double terminator consisting of BBa_B0010 and BBa_B0012
This part is the coding sequence of antigen 43 in K12 strain. Antigen 43 can cause auto-aggregation and specific colony morphology when expressed.
We got the coding sequence of this gene and add a standard RBS(B0034) to replace the original RBS of this gene, which is regulated by other factors in the cell.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1