BBa_K762000 1 BBa_K762000 Translational Coupling Cassette 2012-09-24T11:00:00Z 2015-05-08T01:13:13Z Mendez-Perez, D., S. Gunasekaran, V. J. Orler, and B. F. Pfleger. "A Translation-coupling DNA Cassette for Monitoring Protein Translation in Escherichia Coli." Metabolic Engineering 4 (2012): 298-305. In synthetic biology, a powerful method for the production of novel metabolites is the expression of heterologous genes in Escherichia coli. A common challenge when using non-native genes in metabolic engineering is determining if they are being properly expressed. To address this issue, we have constructed a BioFusion compatible system for testing the translation of a gene of interest. This system couples the translation of the target gene to a fluorescent reporter gene. Fluorescence will only be detected when the target gene is entirely translated. This construct enables synthetic biologists to quickly determine if a gene is being expressed without the need for costly antibodies or analytical instruments (e.g. mass spectrometry). false false _1013_ 0 11672 9 In stock true Adapted design from Mendez-Perez, et al. false Menuka Samaranayake annotation2198657 1 RBS for Response Gene range2198657 1 24 29 annotation2198655 1 His-Tag for fused Target Gene range2198655 1 1 18 annotation2198656 1 Stop Codon for fused Target Gene range2198656 1 19 21 annotation2198658 1 Red Florescent Protein ORF range2198658 1 38 715 annotation2198672 1 mRNA Hairpin range2198672 1 2 36 BBa_K762000_sequence 1 catcatcaccaccatcattaggatggtggtgatgataatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z