LOCUS BBa_K762001 2453 bp DNA linear 23-Dec-2024 DEFINITION TCC Negative control ACCESSION BBa_K762001 VERSION BBa_K762001.1 FEATURES Location/Qualifiers misc_feature 1..35 /label=BBa_J23102 RBS 44..62 /label=RBS misc_feature 63..1727 /label=Limonene Synthase ORF misc_feature 834..836 /label=Embedded Stop 1 misc_feature 867..869 /label=Embedded Stop 2 misc_feature 1736..2453 /label=BBa_K762000 misc_feature 1736..1753 /label=His-Tag for fused Target Gene stem_loop 1737..1771 /label=mRNA Hairpin misc_feature 1754..1756 /label=Stop Codon for fused Target Gene RBS 1759..1764 /label=RBS for Response Gene misc_feature 1773..2450 /label=Red Florescent Protein ORF ORIGIN 1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gaggaaagag gagaaatact 61 agatgcgtcg tagcgcgaat tatcaacctt caatttggga ccacgacttt ttacaaagcc 121 tgaacagcaa ctatacggac gaagcttata aacgtcgcgc agaagagctg cgcggaaaag 181 ttaaaattgc gatcaaagat gttattgaac cgctggacca acttgagctg attgataacc 241 tgcaacgtct gggcctcgcg catcgcttcg aaacagaaat tcgcaatatc ttgaacaata 301 tttataacaa taataaagac tataattggc gtaaggagaa cttgtatgcg acatcgctgg 361 agttccgtct gttacgccag catggctacc cagtcagcca ggaagtcttt aacggtttta 421 aggatgacca aggtggtttt atttgtgatg attttaaagg gattctgtcg ttacacgaag 481 cctcttacta tagtttggaa ggggaaagca tcatggaaga agcatggcaa ttcacttcga 541 aacatctgaa agaagtcatg attagcaaaa acatggaaga ggacgttttc gtagcagaac 601 aagcgaaacg cgctctggag ctgcctctgc attggaaggt acccatgctg gaagcccgct 661 ggtttattca catctatgag cgtcgtgagg ataaaaacca tcttctgctg gagctcgcca 721 aaatggaatt taacacgttg caggctattt atcaggaaga actgaaggaa atctcgggtt 781 ggtggaaaga taccgggctg ggtgagaagt tatcctttgc gcgtacagga tggtgacgcc 841 cgttttatga acacggcgac cgtgtataag cctctttcgc atattgccgc cgtgtgttga 901 ctattagcat tgccctgatc acagtgattg acgacatcta cgacgtatat ggtaccttgg 961 atgagctgga aattttcacg gatgctgtag aacgctggga cattaactat gcgctgaaac 1021 atttaccggg atacatgaag atgtgttttc tggccctgta taattttgtg aatgaatttg 1081 cctattatgt tctcaaacag caagacttcg atctgctgct ttcgattaag aatgcttggc 1141 ttggtctgat tcaggcatat ctggttgaag ccaaatggta tcatagtaag tacactccga 1201 aattagagga atatttagaa aatggacttg tgtctatcac cggcccactg atcattacga 1261 ttagctatct cagcgggacc aatcccatta tcaagaaaga gttggagttt ctggaatcta 1321 atccagatat cgtgcattgg tcatctaaga ttttccgtct gcaagatgat ttgggcacgt 1381 cgagcgacga aattcaacgc ggagatgtgc cgaaatcaat tcaatgttat atgcatgaga 1441 cgggtgcgtc agaagaagtc gcgcgccaac acatcaaaga catgatgcgc cagatgtgga 1501 aaaaagtgaa tgcgtatact gccgacaaag actcgccatt gactggtacc accacggagt 1561 ttctgctgaa tctggtacgt atgtcacatt tcatgtacct gcatggtgac ggtcatggtg 1621 ttcagaatca agaaacaatt gatgtaggtt tcacgctgct gtttcagccg attcctctcg 1681 aggataaaca tatggccttt acagcgagtc ctggaaccaa aggttaatac tagagcatca 1741 tcaccaccat cattaggatg gtggtgatga taatggcttc ctccgaagac gttatcaaag 1801 agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg 1861 aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca 1921 aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca 1981 aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag 2041 gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg 2101 actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc 2161 cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta 2221 tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg 2281 gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc 2341 cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca 2401 tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taa //