BBa_K762001 1 BBa_K762001 TCC Negative control 2012-09-23T11:00:00Z 2015-05-08T01:13:13Z Based off of UW-Madison's BBa_K762100 Negative control for Translational Coupling Cassette by UW-Madison 2012's team. Based off UW-Madison's BBa_K762100 but with a stop codon in the middle of the sequence. This stop codon will prevent the hairpin from going away, preventing translation and florescence. false false _1013_ 0 11672 9 It's complicated false Designed purposely to include a stop codon prematurely in the sequence to prevent translation false Menuka Samaranayake annotation2203886 1 BBa_K762000 range2203886 1 1736 2453 annotation2203885 1 Embedded Stop 2 range2203885 1 867 869 annotation2203884 1 Embedded Stop 1 range2203884 1 834 836 annotation2203888 1 mRNA Hairpin range2203888 1 1737 1771 annotation2203890 1 RBS for Response Gene range2203890 1 1759 1764 annotation2203882 1 RBS range2203882 1 44 62 annotation2203887 1 His-Tag for fused Target Gene range2203887 1 1736 1753 annotation2203891 1 Red Florescent Protein ORF range2203891 1 1773 2450 annotation2203881 1 BBa_J23102 range2203881 1 1 35 annotation2203883 1 Limonene Synthase ORF range2203883 1 63 1727 annotation2203889 1 Stop Codon for fused Target Gene range2203889 1 1754 1756 BBa_K762001_sequence 1 ttgacagctagctcagtcctaggtactgtgctagctactagaggaaagaggagaaatactagatgcgtcgtagcgcgaattatcaaccttcaatttgggaccacgactttttacaaagcctgaacagcaactatacggacgaagcttataaacgtcgcgcagaagagctgcgcggaaaagttaaaattgcgatcaaagatgttattgaaccgctggaccaacttgagctgattgataacctgcaacgtctgggcctcgcgcatcgcttcgaaacagaaattcgcaatatcttgaacaatatttataacaataataaagactataattggcgtaaggagaacttgtatgcgacatcgctggagttccgtctgttacgccagcatggctacccagtcagccaggaagtctttaacggttttaaggatgaccaaggtggttttatttgtgatgattttaaagggattctgtcgttacacgaagcctcttactatagtttggaaggggaaagcatcatggaagaagcatggcaattcacttcgaaacatctgaaagaagtcatgattagcaaaaacatggaagaggacgttttcgtagcagaacaagcgaaacgcgctctggagctgcctctgcattggaaggtacccatgctggaagcccgctggtttattcacatctatgagcgtcgtgaggataaaaaccatcttctgctggagctcgccaaaatggaatttaacacgttgcaggctatttatcaggaagaactgaaggaaatctcgggttggtggaaagataccgggctgggtgagaagttatcctttgcgcgtacaggatggtgacgcccgttttatgaacacggcgaccgtgtataagcctctttcgcatattgccgccgtgtgttgactattagcattgccctgatcacagtgattgacgacatctacgacgtatatggtaccttggatgagctggaaattttcacggatgctgtagaacgctgggacattaactatgcgctgaaacatttaccgggatacatgaagatgtgttttctggccctgtataattttgtgaatgaatttgcctattatgttctcaaacagcaagacttcgatctgctgctttcgattaagaatgcttggcttggtctgattcaggcatatctggttgaagccaaatggtatcatagtaagtacactccgaaattagaggaatatttagaaaatggacttgtgtctatcaccggcccactgatcattacgattagctatctcagcgggaccaatcccattatcaagaaagagttggagtttctggaatctaatccagatatcgtgcattggtcatctaagattttccgtctgcaagatgatttgggcacgtcgagcgacgaaattcaacgcggagatgtgccgaaatcaattcaatgttatatgcatgagacgggtgcgtcagaagaagtcgcgcgccaacacatcaaagacatgatgcgccagatgtggaaaaaagtgaatgcgtatactgccgacaaagactcgccattgactggtaccaccacggagtttctgctgaatctggtacgtatgtcacatttcatgtacctgcatggtgacggtcatggtgttcagaatcaagaaacaattgatgtaggtttcacgctgctgtttcagccgattcctctcgaggataaacatatggcctttacagcgagtcctggaaccaaaggttaatactagagcatcatcaccaccatcattaggatggtggtgatgataatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z