BBa_K763004 1 BBa_K763004 pLexA + Gene encoding ZsGreen1 2012-09-06T11:00:00Z 2015-05-08T01:13:13Z The sequence of the promoter comes from E. coli genome, whereas the sequence encoding the AmCyan fluorescent protein is not natural, since it was optimized for codon usage and maximum fluorescence production. When E.coli cells are irradiated with UV light (240 nm), bacteria start synthesizing the fluorescent protein. Why? Eubacteria respond to DNA damage with a set of mechanisms called SOS response. The induction of this system involves more than forty independent SOS genes, most of which encode proteins engaged in protection, repairing, replication, mutagenesis and metabolism of DNA. How does this system work? Under normal conditions, LexA protein represses the transcription of many DNA repairing proteins by binding to some sequences called SOS ???boxes??? in their operators. But, when a damage in the DNA occurs, (different types of genotoxic stress can make this system start), the accumulation RecA-ssDNA-ATP complexes activate LexA for autocleavage. false false _1014_ 0 11756 9 It's complicated false No design considerations needed. false Pedro Luis Dorado Morales BBa_K763004_sequence 1 aaaacacttgatactgtatgagcatacagtataattgcttcaacagaacatattgactatccggtattacccggcatgacaggagtaaaaatggaagggtgcgtcgatggacataaatttgtgatcacgggagagggcattggatatccgttcaaagggaaacaggctattaatctgtgtgtggtcgaaggtggaccattgccatttgccgaagacatattgtcagctgcctttatgtacggaaacagggttttcactgaatatcctcaagacatagctgactatttcaagaactcgtgtcctgctggttatacatgggacaggtcttttctctttgaggatggagcagtttgcatatgtaatgcagatataacagtgagtgttgaagaaaactgcatgtatcatgagtccaaattttatggagtgaattttcctgctgatggacctgtgatgaaaaagatgacagataactgggagccatcctgcgagaagatcataccagtacctaagcaggggatattgaaaggggatgtctccatgtacctccttctgaaggatggtgggcgtttacggtgccaattcgacacagtttacaaagcaaagtctgtgccaagaaagatgccggactggcacttcatccagcataagctcacccgtgaagaccgcagcgatgctaagaatcagaaatggcatctgacagaacatgctattgcatccggatctgcattgccctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z