BBa_B0014 1 BBa_B0014 double terminator (B0012-B0011) 2003-07-15T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0012 and BBa_B0011 false true _1_ 0 24 7 In stock false true Reshma Shetty component939311 1 BBa_B0011 component939303 1 BBa_B0012 annotation939303 1 BBa_B0012 range939303 1 1 41 annotation939311 1 BBa_B0011 range939311 1 50 95 BBa_K764025 1 BBa_K764025 Hyaluronan synthase + BBa_B0014 2012-09-15T11:00:00Z 2015-05-08T01:13:14Z <partinfo>BBa_K764022</partinfo> + <partinfo> BBa_B0014</partinfo> Released HQ 2013 Gene encoding Hyaluronan synthase with terminator attached false false _1015_ 0 12505 9 In stock false 3A assembly false Frederic Dudbridge component2202485 1 BBa_K764022 component2202492 1 BBa_B0014 annotation2202492 1 BBa_B0014 range2202492 1 1272 1366 annotation2202485 1 BBa_K764022 range2202485 1 1 1263 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation7019 1 BBa_B0011 range7019 1 1 46 annotation1683 1 stem_loop range1683 1 13 35 BBa_K764022 1 BBa_K764022 Hyaluronan synthase 2012-09-15T11:00:00Z 2015-05-08T01:13:13Z GenBank accession number and the paper reference Gene encoding Hyaluronan synthases false false _1015_ 0 12505 9 In stock false Synthesised with the correct biobrick primers false Frederic Dudbridge BBa_K764022_sequence 1 atgccaattttcaaaaagactctcatcgtattgtcctttatcttcctgatctccattctgatctacctgaacatgtacctgttcggcaccagcaccgtgggtatctatggtgtgattctgatcacctacctggtgattaagttgggtctgagctttctgtatgagccgttcaaaggcaagccgcatgactacaaagtcgcagcagttattccgagctacaatgaagatgcggagagcctgctggaaaccctgaaatctgtcctggcgcagacctacccgctgagcgagatttacatcgttgatgacggttccagcaatactgacgccattcaactgatcgaagagtacgtgaaccgcgaagtcgacatttgccgcaatgtgattgttcatcgtagcttggtcaataagggtaaacgccacgctcaggcgtgggcgtttgagcgttcggacgctgacgtgtttttgaccgttgatagcgatacctatatctaccctaacgcgctggaagagttgctgaagtccttcaacgacgaaaccgtctatgcggccacgggtcatctgaacgcacgtaaccgtcaaaccaacttgctgacccgtctgaccgatatccgctatgacaatgcgtttggcgttgaacgtgcagcgcagtcgctgacgggtaatatcctggtctgtagcggtccgctgagcatctaccgtcgtgaagtgattattccaaatctggagcgttataagaaccaaacgttcctgggtctgccggtgagcattggtgacgaccgttgcctgacgaactacgcaattgatttgggccgtacggtttatcagtctacggcacgctgcgatacggatgtgccgttccaactgaaaagctatctgaaacaacagaaccgctggaataagagctttttccgtgagagcatcatcagcgtcaagaaaattctgagcaatccgatcgttgctctgtggaccatttttgaggttgttatgttcatgatgctgattgtggcgattggcaatctgctgtttaaccaggccatccagttggatctgatcaaattgttcgcgtttctgagcatcattttcatcgttgccctgtgtcgcaacgtgcactatatggttaagcacccggcgtcttttctgctgagcccgctgtacggcatcctgcacctgttcgtcctgcaaccgctgaaactgtacagcctgtgtaccattaagaataccgagtggggcacgcgtaagaaagtcactattttcaaataataa BBa_B0014_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K764025_sequence 1 atgccaattttcaaaaagactctcatcgtattgtcctttatcttcctgatctccattctgatctacctgaacatgtacctgttcggcaccagcaccgtgggtatctatggtgtgattctgatcacctacctggtgattaagttgggtctgagctttctgtatgagccgttcaaaggcaagccgcatgactacaaagtcgcagcagttattccgagctacaatgaagatgcggagagcctgctggaaaccctgaaatctgtcctggcgcagacctacccgctgagcgagatttacatcgttgatgacggttccagcaatactgacgccattcaactgatcgaagagtacgtgaaccgcgaagtcgacatttgccgcaatgtgattgttcatcgtagcttggtcaataagggtaaacgccacgctcaggcgtgggcgtttgagcgttcggacgctgacgtgtttttgaccgttgatagcgatacctatatctaccctaacgcgctggaagagttgctgaagtccttcaacgacgaaaccgtctatgcggccacgggtcatctgaacgcacgtaaccgtcaaaccaacttgctgacccgtctgaccgatatccgctatgacaatgcgtttggcgttgaacgtgcagcgcagtcgctgacgggtaatatcctggtctgtagcggtccgctgagcatctaccgtcgtgaagtgattattccaaatctggagcgttataagaaccaaacgttcctgggtctgccggtgagcattggtgacgaccgttgcctgacgaactacgcaattgatttgggccgtacggtttatcagtctacggcacgctgcgatacggatgtgccgttccaactgaaaagctatctgaaacaacagaaccgctggaataagagctttttccgtgagagcatcatcagcgtcaagaaaattctgagcaatccgatcgttgctctgtggaccatttttgaggttgttatgttcatgatgctgattgtggcgattggcaatctgctgtttaaccaggccatccagttggatctgatcaaattgttcgcgtttctgagcatcattttcatcgttgccctgtgtcgcaacgtgcactatatggttaagcacccggcgtcttttctgctgagcccgctgtacggcatcctgcacctgttcgtcctgcaaccgctgaaactgtacagcctgtgtaccattaagaataccgagtggggcacgcgtaagaaagtcactattttcaaataataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z