##gff-version 3
##sequence-region BBa_K764033 1 1731
BBa_K764033	.	engineered_region	83	1731	.	+	0	ID=BBa_K764028;Name=BBa_K764028
BBa_K764033	.	engineered_region	192	1731	.	+	0	ID=BBa_K764024;Name=BBa_K764024;Parent=BBa_K764028
BBa_K764033	.	engineered_region	192	1628	.	+	0	ID=BBa_K322921;Name=BBa_K322921;Parent=BBa_K764024
BBa_K764033	.	ribosome_entry_site	192	194	.	+	0	ID=annotation2098459;Name=RBS;Parent=BBa_K322921
BBa_K764033	.	point_mutation	402	407	.	+	0	ID=annotation2098481;Name=Removed EcoRI site;Parent=BBa_K322921
BBa_K764033	.	CDS	204	1625	.	+	0	ID=annotation2098480;Name=Coding sequence;Parent=BBa_K322921
BBa_K764033	.	terminator	1637	1731	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K764024
BBa_K764033	.	terminator	1686	1731	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K764033	.	engineered_region	1686	1731	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K764033	.	stem_loop	1698	1720	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K764033	.	terminator	1637	1677	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K764033	.	stem_loop	1644	1663	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K764033	.	stop_codon	1670	1670	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K764033	.	polyA_site	1664	1677	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K764033	.	engineered_region	1637	1677	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K764033	.	engineered_region	83	183	.	+	0	ID=BBa_K764027;Name=BBa_K764027;Parent=BBa_K764028
BBa_K764033	.	promoter	1	74	.	+	0	ID=BBa_J13002;Name=BBa_J13002
BBa_K764033	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J13002
BBa_K764033	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K764033	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J13002
BBa_K764033	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K764033	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K764033	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K764033	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K764033	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
>BBa_K764033
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gagaattcgcggccgcttctagatgaagaagacagcaatagccattgcagtcgcacttgcaggattcgcgaccgtcgcc
caagccactagtagcggccgctgcatactagaggagacatgaacgatgaacatcaaaaagtttgcaaaacaagcaacag
tattaacctttactaccgcactgctggcaggaggcgcaactcaagcgtttgcgaaagaaacgaaccaaaagccatataa
ggaaacatacggcatttcccatattacacgccatgatatgctgcaaatccctgaacagcaaaaaaatgaaaaatatcaa
gttcctgagttcgattcgtccacaattaaaaatatctcttctgcaaaaggcctggacgtttgggacagctggccattac
aaaacgctgacggcactgtcgcaaactatcacggctaccacatcgtctttgcattagccggagatcctaaaaatgcgga
tgacacatcgatttacatgttctatcaaaaagtcggcgaaacttctattgacagctggaaaaacgctggccgcgtcttt
aaagacagcgacaaattcgatgcaaatgattctatcctaaaagaccaaacacaagaatggtcaggttcagccacattta
catctgacggaaaaatccgtttattctacactgatttctccggtaaacattacggcaaacaaacactgacaactgcaca
agttaacgtatcagcatcagacagctctttgaacatcaacggtgtagaggattataaatcaatctttgacggtgacgga
aaaacgtatcaaaatgtacagcagttcatcgatgaaggcaactacagctcaggcgacaaccatacgctgagagatcctc
actacgtagaagataaaggccacaaatacttagtatttgaagcaaacactggaactgaagatggctaccaaggcgaaga
atctttatttaacaaagcatactatggcaaaagcacatcattcttccgtcaagaaagtcaaaaacttctgcaaagcgat
aaaaaacgcacggctgagttagcaaacggcgctctcggtatgattgagctaaacgatgattacacactgaaaaaagtga
tgaaaccgctgattgcatctaacacagtaacagatgaaattgaacgcgcgaacgtctttaaaatgaacggcaaatggta
cctgttcactgactcccgcggatcaaaaatgacgattgacggcattacgtctaacgatatttacatgcttggttatgtt
tctaattctttaactggcccatacaagccgctgaacaaaactggccttgtgttaaaaatggatcttgatcctaacgatg
taacctttacttactcacacttcgctgtacctcaagcgaaaggaaacaatgtcgtgattacaagctatatgacaaacag
aggattctacgcagacaaacaatcaacgtttgcgccaagcttcctgctgaacatcaaaggcaagaaaacatctgttgtc
aaagacagcatccttgaacaaggacaattaacagttaacaaataataatactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt