LOCUS       BBa_K764034             1780 bp    DNA     linear       29-Nov-2024
DEFINITION  Pbad Strong_rbs_ompA-sacB-Term
ACCESSION   BBa_K764034
VERSION     BBa_K764034.1  
FEATURES             Location/Qualifiers
     promoter        1..103
                     /label=pLac/ara-1
     promoter        1..103
                     /label=BBa_K094120
     misc_feature    116..119
                     /label=conserved
     RBS             112..123
                     /label=BBa_B0034
     misc_feature    1..123
                     /label=BBa_K764001
     misc_feature    132..232
                     /label=BBa_K764027
     RBS             241..243
                     /label=RBS
     CDS             253..1674
                     /label=Coding sequence
     misc_feature    451..456
                     /label=Removed EcoRI site
     misc_feature    241..1677
                     /label=BBa_K322921
     stem_loop       1693..1712
                     /label=T7 TE
     misc_feature    1719..1719
                     /label=stop
     polyA_site      1713..1726
                     /label=polya
     misc_feature    1686..1726
                     /label=BBa_B0012
     terminator      1686..1726
                     /label=BBa_B0012
     stem_loop       1747..1769
                     /label=stem_loop
     misc_feature    1735..1780
                     /label=BBa_B0011
     terminator      1735..1780
                     /label=BBa_B0011
     terminator      1686..1780
                     /label=BBa_B0014
     misc_feature    241..1780
                     /label=BBa_K764024
     misc_feature    132..1780
                     /label=BBa_K764028
ORIGIN
        1 catagcattt ttatccataa gattagcgga tctaaccttt acaattgtga gcgctcacaa
       61 ttatgataga ttcaattgtg agcggataac aatttcacac agatactaga gaaagaggag
      121 aaatactaga gaattcgcgg ccgcttctag atgaagaaga cagcaatagc cattgcagtc
      181 gcacttgcag gattcgcgac cgtcgcccaa gccactagta gcggccgctg catactagag
      241 gagacatgaa cgatgaacat caaaaagttt gcaaaacaag caacagtatt aacctttact
      301 accgcactgc tggcaggagg cgcaactcaa gcgtttgcga aagaaacgaa ccaaaagcca
      361 tataaggaaa catacggcat ttcccatatt acacgccatg atatgctgca aatccctgaa
      421 cagcaaaaaa atgaaaaata tcaagttcct gagttcgatt cgtccacaat taaaaatatc
      481 tcttctgcaa aaggcctgga cgtttgggac agctggccat tacaaaacgc tgacggcact
      541 gtcgcaaact atcacggcta ccacatcgtc tttgcattag ccggagatcc taaaaatgcg
      601 gatgacacat cgatttacat gttctatcaa aaagtcggcg aaacttctat tgacagctgg
      661 aaaaacgctg gccgcgtctt taaagacagc gacaaattcg atgcaaatga ttctatccta
      721 aaagaccaaa cacaagaatg gtcaggttca gccacattta catctgacgg aaaaatccgt
      781 ttattctaca ctgatttctc cggtaaacat tacggcaaac aaacactgac aactgcacaa
      841 gttaacgtat cagcatcaga cagctctttg aacatcaacg gtgtagagga ttataaatca
      901 atctttgacg gtgacggaaa aacgtatcaa aatgtacagc agttcatcga tgaaggcaac
      961 tacagctcag gcgacaacca tacgctgaga gatcctcact acgtagaaga taaaggccac
     1021 aaatacttag tatttgaagc aaacactgga actgaagatg gctaccaagg cgaagaatct
     1081 ttatttaaca aagcatacta tggcaaaagc acatcattct tccgtcaaga aagtcaaaaa
     1141 cttctgcaaa gcgataaaaa acgcacggct gagttagcaa acggcgctct cggtatgatt
     1201 gagctaaacg atgattacac actgaaaaaa gtgatgaaac cgctgattgc atctaacaca
     1261 gtaacagatg aaattgaacg cgcgaacgtc tttaaaatga acggcaaatg gtacctgttc
     1321 actgactccc gcggatcaaa aatgacgatt gacggcatta cgtctaacga tatttacatg
     1381 cttggttatg tttctaattc tttaactggc ccatacaagc cgctgaacaa aactggcctt
     1441 gtgttaaaaa tggatcttga tcctaacgat gtaaccttta cttactcaca cttcgctgta
     1501 cctcaagcga aaggaaacaa tgtcgtgatt acaagctata tgacaaacag aggattctac
     1561 gcagacaaac aatcaacgtt tgcgccaagc ttcctgctga acatcaaagg caagaaaaca
     1621 tctgttgtca aagacagcat ccttgaacaa ggacaattaa cagttaacaa ataataatac
     1681 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagagagaa
     1741 tataaaaagc cagattatta atccggcttt tttattattt
//