BBa_K766006 1 BBa_K766006 AHL activated Plux-LacI 2012-09-23T11:00:00Z 2015-05-08T01:13:14Z We get LacI from the plasmid of our lab. Then we use overlap PCR to add the Plux promoter This device is a protein-expression unit which contains a Plux promoter and a transcriptional unit of LacI without LVA-tag. Plux can be activated by luxR-AHL complex and the promoter can activate the expression of downstream genes. We add LacI in this part. As we all know, LacI is a repressor which can bind with Lac operator. With the help of this feature, many logic gates or gene expression modulation can be achieved. There is not LVA-tag in this part so that the repressor can keep working for a relatively long time. false false _1018_ 0 8494 9 Not in stock false We want to design a part that can repress another gene's expression false Yu Zhao annotation2195320 1 RBS range2195320 1 64 75 annotation2195318 1 Plux promoter range2195318 1 1 55 annotation2195323 1 LacI repressor range2195323 1 82 1170 BBa_K766006_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatgtttaacaaagaggagaaatgatacatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z