LOCUS       BBa_K769013             2447 bp    DNA     linear       23-Dec-2024
DEFINITION  Promoter(Pbad)-RBS-luxA-DT
ACCESSION   BBa_K769013
VERSION     BBa_K769013.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..2310
                     /label=BBa_K769100
     stem_loop       2330..2373
                     /label=stem_loop
     misc_feature    2319..2398
                     /label=BBa_B0010
     terminator      2319..2398
                     /label=BBa_B0010
     stem_loop       2414..2433
                     /label=T7 TE
     misc_feature    2440..2440
                     /label=stop
     polyA_site      2434..2447
                     /label=polya
     misc_feature    2407..2447
                     /label=BBa_B0012
     terminator      2407..2447
                     /label=BBa_B0012
     terminator      2319..2447
                     /label=BBa_B0015
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga agtttggaaa tatttgtttc
     1261 tcatatcagc ctccaggtga gtcacataaa gaagtcatgg atcgctttgt tcgtttaggc
     1321 gttgcatcag aagaactaaa ttttgatact tactggactc tcgagcatca ttttactgaa
     1381 tttggactaa caggtaacct gtttgttgct tgtgctaact tacttggtcg aaccaccaaa
     1441 ctgaatgttg gtactatggg tattgttctt ccaacagctc accctgcacg tcagatggaa
     1501 gatttattac ttttagatca aatgtcaaaa ggccgtttta attttggtgt tgtgcgtggc
     1561 ttgtaccaca aagatttccg cgtttttggt gtaacgatgg aagattctcg tgctatcact
     1621 gaagattttc acaccatgat tatggatggt acaaaaacag gtacacttca tactgatggt
     1681 aaaaacatcg agttcccaga tgtaaacgtt tacccagagg cgtatttaga gaaaattcca
     1741 acatgcatga ctgctgaatc agcagtaaca acgacttggc ttgctgagcg tggcttaccg
     1801 atggttctta gttggattat tacaacgagt gaaaagaaag ctcaaatgga actctataat
     1861 gctgttgctc gagatagcgg ttacagtgaa gagtacatta aaaacgttga tcacagtatg
     1921 accctcatct gttctgtaga tgaagatggc aaaaaagctg aagatgtgtg ccgtgagttt
     1981 ttaggtaatt ggtatgattc atacgtaaat gcaaccaata tctttagtga aagtaaccaa
     2041 actcgtggtt atgattatca taaaggtcaa tggaaagatt ttgttcttca aggacatact
     2101 aatactaaac gtcgtgttga ttatagcaac gatctaaacc ctgtaggtac acctgaaaaa
     2161 tgtattgaaa ttattcagcg tgatattgat gcaacaggta ttactaatat tacccttggt
     2221 ttcgaagcaa atggctctga ggaagaaatc attgcctcta tgaaacgctt catgacgcaa
     2281 gttgcaccat tcttaaaaga tccaaaataa tactagagcc aggcatcaaa taaaacgaaa
     2341 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     2401 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//