BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K769100 1 BBa_K769100 luxA 2012-09-18T11:00:00Z 2015-05-08T01:13:14Z Photobacterium kishitanii strain NCMB 844 lux-rib operon luxA false false _1021_ 0 14229 9 Not in stock false luxA false Mayumi Nakamura BBa_K769013 1 BBa_K769013 Promoter(Pbad)-RBS-luxA-DT 2012-09-18T11:00:00Z 2015-05-08T01:13:14Z Photobacterium kishitanii strain NCMB 844 lux-rib operon Promoter(Pbad)-RBS-luxA-DT false false _1021_ 0 14229 9 Not in stock false Promoter(Pbad)-RBS-luxA-DT false Mayumi Nakamura component2186543 1 BBa_B0034 component2186551 1 BBa_B0015 component2186541 1 BBa_I0500 component2186544 1 BBa_K769100 annotation2186551 1 BBa_B0015 range2186551 1 2319 2447 annotation2186541 1 BBa_I0500 range2186541 1 1 1210 annotation2186543 1 BBa_B0034 range2186543 1 1219 1230 annotation2186544 1 BBa_K769100 range2186544 1 1237 2310 BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498841 1 Pbad range1498841 1 1080 1210 annotation1498843 1 AraC range1498843 1 1 1079 annotation1498842 1 PC range1498842 1 1043 1114 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K769013_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgaagtttggaaatatttgtttctcatatcagcctccaggtgagtcacataaagaagtcatggatcgctttgttcgtttaggcgttgcatcagaagaactaaattttgatacttactggactctcgagcatcattttactgaatttggactaacaggtaacctgtttgttgcttgtgctaacttacttggtcgaaccaccaaactgaatgttggtactatgggtattgttcttccaacagctcaccctgcacgtcagatggaagatttattacttttagatcaaatgtcaaaaggccgttttaattttggtgttgtgcgtggcttgtaccacaaagatttccgcgtttttggtgtaacgatggaagattctcgtgctatcactgaagattttcacaccatgattatggatggtacaaaaacaggtacacttcatactgatggtaaaaacatcgagttcccagatgtaaacgtttacccagaggcgtatttagagaaaattccaacatgcatgactgctgaatcagcagtaacaacgacttggcttgctgagcgtggcttaccgatggttcttagttggattattacaacgagtgaaaagaaagctcaaatggaactctataatgctgttgctcgagatagcggttacagtgaagagtacattaaaaacgttgatcacagtatgaccctcatctgttctgtagatgaagatggcaaaaaagctgaagatgtgtgccgtgagtttttaggtaattggtatgattcatacgtaaatgcaaccaatatctttagtgaaagtaaccaaactcgtggttatgattatcataaaggtcaatggaaagattttgttcttcaaggacatactaatactaaacgtcgtgttgattatagcaacgatctaaaccctgtaggtacacctgaaaaatgtattgaaattattcagcgtgatattgatgcaacaggtattactaatattacccttggtttcgaagcaaatggctctgaggaagaaatcattgcctctatgaaacgcttcatgacgcaagttgcaccattcttaaaagatccaaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc BBa_K769100_sequence 1 atgaagtttggaaatatttgtttctcatatcagcctccaggtgagtcacataaagaagtcatggatcgctttgttcgtttaggcgttgcatcagaagaactaaattttgatacttactggactctcgagcatcattttactgaatttggactaacaggtaacctgtttgttgcttgtgctaacttacttggtcgaaccaccaaactgaatgttggtactatgggtattgttcttccaacagctcaccctgcacgtcagatggaagatttattacttttagatcaaatgtcaaaaggccgttttaattttggtgttgtgcgtggcttgtaccacaaagatttccgcgtttttggtgtaacgatggaagattctcgtgctatcactgaagattttcacaccatgattatggatggtacaaaaacaggtacacttcatactgatggtaaaaacatcgagttcccagatgtaaacgtttacccagaggcgtatttagagaaaattccaacatgcatgactgctgaatcagcagtaacaacgacttggcttgctgagcgtggcttaccgatggttcttagttggattattacaacgagtgaaaagaaagctcaaatggaactctataatgctgttgctcgagatagcggttacagtgaagagtacattaaaaacgttgatcacagtatgaccctcatctgttctgtagatgaagatggcaaaaaagctgaagatgtgtgccgtgagtttttaggtaattggtatgattcatacgtaaatgcaaccaatatctttagtgaaagtaaccaaactcgtggttatgattatcataaaggtcaatggaaagattttgttcttcaaggacatactaatactaaacgtcgtgttgattatagcaacgatctaaaccctgtaggtacacctgaaaaatgtattgaaattattcagcgtgatattgatgcaacaggtattactaatattacccttggtttcgaagcaaatggctctgaggaagaaatcattgcctctatgaaacgcttcatgacgcaagttgcaccattcttaaaagatccaaaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z