LOCUS       BBa_K769014             2294 bp    DNA     linear       26-Nov-2024
DEFINITION  Promoter(Pbad)-RBS-luxD-DT
ACCESSION   BBa_K769014
VERSION     BBa_K769014.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..2157
                     /label=BBa_K769101
     stem_loop       2177..2220
                     /label=stem_loop
     misc_feature    2166..2245
                     /label=BBa_B0010
     terminator      2166..2245
                     /label=BBa_B0010
     stem_loop       2261..2280
                     /label=T7 TE
     misc_feature    2287..2287
                     /label=stop
     polyA_site      2281..2294
                     /label=polya
     misc_feature    2254..2294
                     /label=BBa_B0012
     terminator      2254..2294
                     /label=BBa_B0012
     terminator      2166..2294
                     /label=BBa_B0015
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga aaagtgaaaa caattctgta
     1261 ccaattgatc atgttataaa agttgataac gaacgtcata tacgtgtttg ggaaactttc
     1321 cctaaaaatc aatgtgataa aagaaataat actattgtta ttgcttctgg ttttgctcga
     1381 agaatggatc attttgcagg tttagctgaa tatttatcaa ccaatggatt tcatgttatt
     1441 cgttatgatt cacttaatca tgttggatta agtagcggtg aaattgatca gttctcaatg
     1501 tcagtaggta agaaaagttt attaaccgtt attgactggt tgaaatcaga gcatggtatt
     1561 gatcaggtag gtttaattgc atcgagtctt tctgctcgaa ttgcttatga tattgtcgct
     1621 gatgttaatt tgtctttttt aattactgcc gttggtgtgg tcaatttacg gaatactctt
     1681 gaacaagcgc ttaaatatga ttacttgcag atggagatag atgaaatacc agaagattta
     1741 gatttcgatg gatataattt aggttcaaag gtatttgtta cagattgttt tgaaaataac
     1801 tgggatacat tagattcaac tataaataaa acgaaaaatt taaatgtccc atttatagct
     1861 tttgtcgcca atgatgatag ttgggtacaa cagcacgaag tcgaagaatt aatgagtaat
     1921 atcaattcag ataaaaccaa gatttactct ttaataggtt catcacatga tttaggtgaa
     1981 aacctaattg tgttaagaaa tttctatcaa tcaattacta aagcagcaat tgcattagat
     2041 agtaatttag tagggttagt aagtgagatt attgaaccac aatttgaagc tctcactatt
     2101 gctacagtaa atgaacgtcg tttgaaaaat aaaatacaaa gtaagtcatt agcttaatac
     2161 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     2221 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     2281 tttctgcgtt tata
//