LOCUS BBa_K769018 8725 bp DNA linear 23-Dec-2024 DEFINITION Promoter(Pbad)-RBS-luxD-DT-Promoter(high)-RBS-lux CABFEG-DT ACCESSION BBa_K769018 VERSION BBa_K769018.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..2157 /label=BBa_K769101 stem_loop 2177..2220 /label=stem_loop misc_feature 2166..2245 /label=BBa_B0010 terminator 2166..2245 /label=BBa_B0010 stem_loop 2261..2280 /label=T7 TE misc_feature 2287..2287 /label=stop polyA_site 2281..2294 /label=polya misc_feature 2254..2294 /label=BBa_B0012 terminator 2254..2294 /label=BBa_B0012 terminator 2166..2294 /label=BBa_B0015 misc_feature 1..2294 /label=BBa_K769014 promoter 2303..2337 /label=BBa_J23100 misc_feature 2350..2353 /label=conserved RBS 2346..2357 /label=BBa_B0034 CDS 2364..8588 /label=BBa_K769103 stem_loop 8608..8651 /label=stem_loop misc_feature 8597..8676 /label=BBa_B0010 terminator 8597..8676 /label=BBa_B0010 stem_loop 8692..8711 /label=T7 TE misc_feature 8718..8718 /label=stop polyA_site 8712..8725 /label=polya misc_feature 8685..8725 /label=BBa_B0012 terminator 8685..8725 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga aaagtgaaaa caattctgta 1261 ccaattgatc atgttataaa agttgataac gaacgtcata tacgtgtttg ggaaactttc 1321 cctaaaaatc aatgtgataa aagaaataat actattgtta ttgcttctgg ttttgctcga 1381 agaatggatc attttgcagg tttagctgaa tatttatcaa ccaatggatt tcatgttatt 1441 cgttatgatt cacttaatca tgttggatta agtagcggtg aaattgatca gttctcaatg 1501 tcagtaggta agaaaagttt attaaccgtt attgactggt tgaaatcaga gcatggtatt 1561 gatcaggtag gtttaattgc atcgagtctt tctgctcgaa ttgcttatga tattgtcgct 1621 gatgttaatt tgtctttttt aattactgcc gttggtgtgg tcaatttacg gaatactctt 1681 gaacaagcgc ttaaatatga ttacttgcag atggagatag atgaaatacc agaagattta 1741 gatttcgatg gatataattt aggttcaaag gtatttgtta cagattgttt tgaaaataac 1801 tgggatacat tagattcaac tataaataaa acgaaaaatt taaatgtccc atttatagct 1861 tttgtcgcca atgatgatag ttgggtacaa cagcacgaag tcgaagaatt aatgagtaat 1921 atcaattcag ataaaaccaa gatttactct ttaataggtt catcacatga tttaggtgaa 1981 aacctaattg tgttaagaaa tttctatcaa tcaattacta aagcagcaat tgcattagat 2041 agtaatttag tagggttagt aagtgagatt attgaaccac aatttgaagc tctcactatt 2101 gctacagtaa atgaacgtcg tttgaaaaat aaaatacaaa gtaagtcatt agcttaatac 2161 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 2221 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 2281 tttctgcgtt tatatactag agttgacggc tagctcagtc ctaggtacag tgctagctac 2341 tagagaaaga ggagaaatac tagatgataa agaaaatccc aatgattatt ggtggcggag 2401 agagggatac ttcagaacat gaatatcgtg aactcacact aaatagctat aaagttagta 2461 tacctatcat taatcaagat gatgttgagg cgattaaatc acaaaatgtg gaaaataatc 2521 taaatatcaa tcagatagtg aatttcttat acactgttgg tcagaaatgg aaaagtgaga 2581 attattctcg tcgactaacc tatattcgtg atttggtaag atttctcgga tattctcctg 2641 aaatggcaaa actagaagct aactggatct caatgatatt gagctcaaaa agtgccttat 2701 atgatattgt tgaaacagag ttaggttctc gtcatattgt agatgaatgg ttacctcagg 2761 gtgattgtta tgtcaaggcc atgccaaaag gaaaatctgt tcatttgcta gccggtaatg 2821 tgcctctatc tggtgttact tctattatta gagcaattct gactaaaaat gaatgtatca 2881 ttaaaacatc atcagctgat ccatttacgg caatagcatt agcttcaagt tttattgata 2941 cagatgaaca ccatccaatt agccgttcaa tgtcggtaat gtattggtct cataacgaag 3001 atattgcaat cccacaacaa attatgaatt gtgctgatgt tgttgttagt tggggtggat 3061 atgatgcaat taaatgggca acagaacata caccggtaaa cgtcgacata ttaaaatttg 3121 ggccgaagaa aagtattgcg attgttgata atcctgtaga tattacagct tctgctattg 3181 gtgtggctca tgatatttgt ttttatgatc agcaggcctg tttttcaacc caagatatct 3241 attatatagg cgataacatt gatgcgtttt ttgatgagct tgtagaacaa ttaaatctat 3301 atatggatat attgccaaaa ggcgatcaaa catttgatga aaaggcatca ttttcattaa 3361 ttgaaaaaga gtgtcaattc gcaaaatata aagttgagaa aggtgataat caatcttggt 3421 tacttgttaa atcaccgcta ggatcttttg gtaatcaacc attagctcga tctgcatata 3481 ttcaccatgt ctccgatata tcagaaataa cgccttatat agaaaataga attactcaaa 3541 ctgtaacagt tactccttgg gagtcatcat ttaaatatag agatgttcta gcctctcatg 3601 gtgctgagcg tattgttgag tcagggatga ataatatttt ccgtgttggt ggtgcgcatg 3661 atggtatgag acctcttcaa cgtttagtta aatatatttc acatgaaaga ccttatacat 3721 atacaaccaa agatgtagcc gtaaaaattg aacaaacacg ttatctcgaa gaagataagt 3781 ttttagtctt tgtaccataa ttacaactga tacataaacc aacaaaagga atatattatg 3841 aagtttggaa atatttgttt ctcatatcag cctccaggtg agtcacataa agaagtcatg 3901 gatcgctttg ttcgtttagg cgttgcatca gaagaactaa attttgatac ttactggact 3961 ctcgagcatc attttactga atttggacta acaggtaacc tgtttgttgc ttgtgctaac 4021 ttacttggtc gaaccaccaa actgaatgtt ggtactatgg gtattgttct tccaacagct 4081 caccctgcac gtcagatgga agatttatta cttttagatc aaatgtcaaa aggccgtttt 4141 aattttggtg ttgtgcgtgg cttgtaccac aaagatttcc gcgtttttgg tgtaacgatg 4201 gaagattctc gtgctatcac tgaagatttt cacaccatga ttatggatgg tacaaaaaca 4261 ggtacacttc atactgatgg taaaaacatc gagttcccag atgtaaacgt ttacccagag 4321 gcgtatttag agaaaattcc aacatgcatg actgctgaat cagcagtaac aacgacttgg 4381 cttgctgagc gtggcttacc gatggttctt agttggatta ttacaacgag tgaaaagaaa 4441 gctcaaatgg aactctataa tgctgttgct cgagatagcg gttacagtga agagtacatt 4501 aaaaacgttg atcacagtat gaccctcatc tgttctgtag atgaagatgg caaaaaagct 4561 gaagatgtgt gccgtgagtt tttaggtaat tggtatgatt catacgtaaa tgcaaccaat 4621 atctttagtg aaagtaacca aactcgtggt tatgattatc ataaaggtca atggaaagat 4681 tttgttcttc aaggacatac taatactaaa cgtcgtgttg attatagcaa cgatctaaac 4741 cctgtaggta cacctgaaaa atgtattgaa attattcagc gtgatattga tgcaacaggt 4801 attactaata ttacccttgg tttcgaagca aatggctctg aggaagaaat cattgcctct 4861 atgaaacgct tcatgacgca agttgcacca ttcttaaaag atccaaaata aataaatcac 4921 tcagattaac tttaataaat aatataagga atataacatg aattttggat tattcttcct 4981 caactttcag cctgaaaata catcgtcaga aacagtttta gataatatga tcaatactgt 5041 ctctttagtt gataaagatt ataaaaactt tacaactgct ttagtcaacg agcaccattt 5101 ttctaaaaat ggtattgtcg gtgctccgat gacagctgca agcttcctat taggactaac 5161 tgaacgttta catattggtt ctttaaatca agtaattaca acgcatcatc cggttcgtat 5221 tgcagaagaa gcaagtttgc ttgatcaaat gtcagacagc cgctttattc taggtctaag 5281 tgattgtgtt aatgattttg agatggattt ctttaaacgg caacgtgact cacagcagct 5341 acaatttgaa gcttgctatg acatcattaa tgaagctatc acaactaatt actgccaagc 5401 taataatgat ttttataact tccctcgtat ctcaattaat cctcattgct taagtaaaga 5461 gaatatgaag caatatattt tggcttctag tgtgagtgtt gttgagtggg ctgctaaaaa 5521 agcgcttcca ctaacatatc gttggagcga tagccttgaa gataaagaga ttctttataa 5581 gcgttattta gaagttgcag caaagcataa tattgacgtt tctaatgtcg agcatcagtt 5641 cccgctgctt gtaaatttaa atcatgatcg tgatgttgct catcaagaag caacggccta 5701 tttagtaagt tatattgctg aagtataccc acatctaaat cagcaacaaa aaattgctga 5761 acttattagc caacatgcga ttggtactga taatgattac tatgaatcaa cattaaatgc 5821 gttagagcgt acaggttcaa aaaatgtatt actttctttt gaatcaatga agaatcatga 5881 tgatgttgta aaagtgatta atatggttaa tgagaagatt caaaagaatt taccaagctc 5941 gtaagtgtaa aggaagcggt gttattatga ataaatggaa ttacggagtc ttcttcgtta 6001 acttttataa taaaggccaa caagagccat caaaaacgat gaataatgca ttagaaacat 6061 tacgtattat tgatgaagat acatctattt atgatgtgat taatattgat gaccactatc 6121 ttgtaaagaa agacagtgaa gataaaaagc tagcgtcttt tattacacta ggagaaaaac 6181 tatatgtgct tgctaccagt gaaaacacag ttgatattgc agcgaaatat gcattaccgt 6241 tagttttcaa atgggatgat ataaatgagg aacgacttaa attgttgagt ttttataatg 6301 catccgcaag taaatataac aagaatatag atttggttcg acaccagctt atgttacatg 6361 tcaatgttaa tgaggcagaa actgtagcaa aagaagaact caaattatat attgaaaact 6421 atgtagcatg tacacagcct agtaatttta atggctcgat tgatagtatt attcagagta 6481 acgtgacagg gagttataaa gactgtttgt catatgtagc gaatcttgct ggtaaatttg 6541 ataatactgt ggacttctta ctttgttttg agtcaatgca agatcaaaat aagaaaaaat 6601 cagtaatgat agatcttaat aatcaagtta ttaagttccg ccaagataat aatctaatct 6661 aatctacaat cattgcctct tataatggca gtgctaattt aaagttctgc cattatattt 6721 aattatattt taaataggat taaacatgac tattacatta gatacttgcg aaaaagatat 6781 tattgtaagt acagagatcg acgatattat ttttacatca tcacctcttg atattactta 6841 cgatgaacaa gaaagaataa agcataaatt aatattagaa tcatttcgtt accactataa 6901 taataatgaa gattataagt ctttctgtaa tactcagggg gtagacgaaa atatttcatc 6961 acttgatgat atccctgttt ttccgacctc aatgtttaag tatgcaaaaa tatgtacagc 7021 agatgagtct aacattgaaa actggttcac aagtagtggt acgagtggtg taaaaagtca 7081 tattgcccgt gatcgtgtaa gtattgaacg tttacttggt tctgtaaatt atggaatgaa 7141 atatcttggt tcatttcatg aaaatcagct agaacttgtt aatatgggac ctgatcgttt 7201 taatgctaaa aatgtttggt ttaagtatgt aatgagtctt gttgagttat tatatccaac 7261 tacatttact gtaaataacg atgaaataga ttttgaactt actattaaaa gtttaaaaga 7321 aatctataat aaaggaaaag gtatttgttt aattggccct ccgtatttca tttatttgct 7381 atgccagtac atgaaagaga atgatattga atttaatgca ggtaatcgca tctttattat 7441 tactggcggt ggttggaaaa ctaagcaaaa acaagcacta aatcgtcaag attttaatca 7501 actattgatg gaaaccttcc atttagcaca tgaaagtcag attcgagata catttaatca 7561 agttgaatta aatacgtgtt tctttgaaga taaccgtcag cgtaagcatg ttccgccatg 7621 ggtttatgca cgtgcacttg atcctgtaac tctaaagcct gttgaagatg gtcaagaagg 7681 gcttattagt tacatggatg catcatcaac gagttatcca acatttatcg ttactgacga 7741 tatcggtata attcatacaa ttaaagatcc agatccgtac caaggcacta cgattgatat 7801 tgtccgtcgt ttgaatacga gagagcagaa agggtgttca ttatcaatgg catcaggctt 7861 gaaatagctt acaaggaaga tgtatgattt taaattgtaa aataattaaa attgaagctt 7921 ctgaatgtaa tatttttaaa gtatttatta agcctgataa gtgtctcaat tttaaagctg 7981 gtcaatatgt tttagcatat ttagatggta aaaaattacc tttctcaatt gctaattgtc 8041 cgacatgtaa tgagcttata gagttacatg ttgggagttc ggtaaaagaa accgcagtta 8101 aatctatttc tcatttttta gatgcttttg tgaatagctc tgaaatacaa atagatgcac 8161 ctcatggtaa tgcttggcta cgtgaagata gtaattctcc attattactt atagctggag 8221 gtactgggtt atcatatatc aatagtattc ttagtaattg tgtaaatagg aatttgcccc 8281 gttctattta tgtttactgg ggagttaata atattgattt attatatgca gacactcaat 8341 taaaagctct ttctagcgat tttaacaatg ttaaatacgt acctgttttg gaaaactttg 8401 acaataattg gtacggaaaa aaaggcaatg ttattgatgc aataatagag gactttggtg 8461 atttatcaga atttgatatc tatgtttgcg gccctcaagg tatgacacgt agtgttcgtg 8521 aaaagttaac atcacttaaa aaagctgata ctgataaaat gtttgccgat gcttttgcat 8581 atatgtgata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 8641 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 8701 tcgggtgggc ctttctgcgt ttata //