BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K769019 1 BBa_K769019 Promoter(high)-lumP-DT 2012-09-18T11:00:00Z 2015-05-08T01:13:14Z P.kishitanii BBa_K769011+Promoter(high)-lumP-DT false false _1021_ 0 14294 9 It's complicated false blue shift false Tomomi YOKOYAMA component2186344 1 BBa_K322007 component2186341 1 BBa_J23100 component2186351 1 BBa_B0015 annotation2186344 1 BBa_K322007 range2186344 1 44 625 annotation2186351 1 BBa_B0015 range2186351 1 634 762 annotation2186341 1 BBa_J23100 range2186341 1 1 35 BBa_K322007 1 BBa_K322007 Lumazine protein (LumP). Causes blue shift of <i>P. phosphoreum</i> luciferase emmision spectrum. 2010-09-25T11:00:00Z 2015-05-08T01:11:59Z Source: genomic DNA of Photobacterium phosphoreum. This is the lumazine protein of Photobacterium phosphoreum. Lumazine protein (LumP) of Photobacterium phosphoreum is a small protein which binds a fluorescent pteridine, 6,7-dimethyl-8-(1'-D-ribityl)lumazine, as cofactor. In P. phosphoreum, it acts to shift the wavelength of light emitted by luciferase, LuxAB, from 495 nm to a shorter wavelength, around 475 nm (O'Kane et al, 1990 and references cited therein). false false _441_ 0 6225 9 It's complicated true We submitted a version of lumP last year (BBa_K216007), but made a new one since we were having some issues working with our clone (lots of red colonies). So we made a new one in pSB1C3 starting from the original PCR product. On sequencing this (as well as looking at the sequence results from the original plasmids, M1117 and M1118) it was found that the sequence differed from the one we had submitted in a number of mutations, some silent, some not. false Chris French and Maria Kowal annotation2083462 1 rbs range2083462 1 1 9 annotation2083463 1 lumP range2083463 1 10 578 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_K322007_sequence 1 gagattattatgttcaaaggtatagttcagggcactggaataattaaaaaaatatcaaaaaatgatgatacccaaagacatggcattacttttccaaaaaatatattggacttagttgcaaaagatactgtcatgctcgtaaatggatgctcattaactgttgttcgcatcacaggtgatgtcgtttatttcgatatagatcaggcacttaataccacaacctttgattcattagaaatcggtgacaaggtaaacttagaactacgtccagaatttggctcactccttgggaaaggtgctttaactggaaatataaagggggtagctactgttgataatattactgaagaagaagatcttcttaaagtttatattgaaattcctaaaaacctaaccaaaaatatttccgcagaagatcatattggaattaatggtgtatctaattctattgaagaagtttccaatgatattatttgcattaattatcccaaaaatttatctattaccaccaaccttagcactttagaagcaggttcgaaggttaacgtagaaacattaaatgtatcaaatgaatggtaataa BBa_K769019_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagaggagattattatgttcaaaggtatagttcagggcactggaataattaaaaaaatatcaaaaaatgatgatacccaaagacatggcattacttttccaaaaaatatattggacttagttgcaaaagatactgtcatgctcgtaaatggatgctcattaactgttgttcgcatcacaggtgatgtcgtttatttcgatatagatcaggcacttaataccacaacctttgattcattagaaatcggtgacaaggtaaacttagaactacgtccagaatttggctcactccttgggaaaggtgctttaactggaaatataaagggggtagctactgttgataatattactgaagaagaagatcttcttaaagtttatattgaaattcctaaaaacctaaccaaaaatatttccgcagaagatcatattggaattaatggtgtatctaattctattgaagaagtttccaatgatattatttgcattaattatcccaaaaatttatctattaccaccaaccttagcactttagaagcaggttcgaaggttaacgtagaaacattaaatgtatcaaatgaatggtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z