LOCUS BBa_K769022 9476 bp DNA linear 22-Nov-2024 DEFINITION lux operon(K769011) + GFP(I13522) ACCESSION BBa_K769022 VERSION BBa_K769022.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 misc_feature 1237..2673 /label=luxC misc_feature 2686..3606 /label=luxD misc_feature 3644..4717 /label=luxA misc_feature 4764..5750 /label=luxB misc_feature 5773..6468 /label=luxF misc_feature 6552..7673 /label=luxE misc_feature 7690..8394 /label=luxG misc_feature 1237..8394 /label=BBa_K769010 stem_loop 8414..8457 /label=stem_loop misc_feature 8403..8482 /label=BBa_B0010 terminator 8403..8482 /label=BBa_B0010 stem_loop 8498..8517 /label=T7 TE misc_feature 8524..8524 /label=stop polyA_site 8518..8531 /label=polya misc_feature 8491..8531 /label=BBa_B0012 terminator 8491..8531 /label=BBa_B0012 terminator 8403..8531 /label=BBa_B0015 misc_feature 1..8531 /label=BBa_K769011 misc_feature 8540..8558 /label=TetR 1 misc_feature 8540..8593 /label=BBa_R0040 promoter 8559..8564 /label=-35 misc_feature 8565..8583 /label=TetR 2 promoter 8582..8587 /label=-10 promoter 8540..8593 /label=BBa_R0040 misc_feature 8606..8609 /label=conserved RBS 8602..8613 /label=BBa_B0034 CDS 8620..9339 /label=GFP protein CDS 8620..9339 /label=BBa_E0040 stem_loop 9359..9402 /label=stem_loop misc_feature 9348..9427 /label=BBa_B0010 terminator 9348..9427 /label=BBa_B0010 stem_loop 9443..9462 /label=T7 TE misc_feature 9469..9469 /label=stop polyA_site 9463..9476 /label=polya misc_feature 9436..9476 /label=BBa_B0012 terminator 9436..9476 /label=BBa_B0012 misc_feature 8540..9476 /label=BBa_I13522 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga taaagaaaat cccaatgatt 1261 attggtggcg gagagaggga tacttcagaa catgaatatc gtgaactcac actaaatagc 1321 tataaagtta gtatacctat cattaatcaa gatgatgttg aggcgattaa atcacaaaat 1381 gtggaaaata atctaaatat caatcagata gtgaatttct tatacactgt tggtcagaaa 1441 tggaaaagtg agaattattc tcgtcgacta acctatattc gtgatttggt aagatttctc 1501 ggatattctc ctgaaatggc aaaactagaa gctaactgga tctcaatgat attgagctca 1561 aaaagtgcct tatatgatat tgttgaaaca gagttaggtt ctcgtcatat tgtagatgaa 1621 tggttacctc agggtgattg ttatgtcaag gccatgccaa aaggaaaatc tgttcatttg 1681 ctagccggta atgtgcctct atctggtgtt acttctatta ttagagcaat tctgactaaa 1741 aatgaatgta tcattaaaac atcatcagct gatccattta cggcaatagc attagcttca 1801 agttttattg atacagatga acaccatcca attagccgtt caatgtcggt aatgtattgg 1861 tctcataacg aagatattgc aatcccacaa caaattatga attgtgctga tgttgttgtt 1921 agttggggtg gatatgatgc aattaaatgg gcaacagaac atacaccggt aaacgtcgac 1981 atattaaaat ttgggccgaa gaaaagtatt gcgattgttg ataatcctgt agatattaca 2041 gcttctgcta ttggtgtggc tcatgatatt tgtttttatg atcagcaggc ctgtttttca 2101 acccaagata tctattatat aggcgataac attgatgcgt tttttgatga gcttgtagaa 2161 caattaaatc tatatatgga tatattgcca aaaggcgatc aaacatttga tgaaaaggca 2221 tcattttcat taattgaaaa agagtgtcaa ttcgcaaaat ataaagttga gaaaggtgat 2281 aatcaatctt ggttacttgt taaatcaccg ctaggatctt ttggtaatca accattagct 2341 cgatctgcat atattcacca tgtctccgat atatcagaaa taacgcctta tatagaaaat 2401 agaattactc aaactgtaac agttactcct tgggagtcat catttaaata tagagatgtt 2461 ctagcctctc atggtgctga gcgtattgtt gagtcaggga tgaataatat tttccgtgtt 2521 ggtggtgcgc atgatggtat gagacctctt caacgtttag ttaaatatat ttcacatgaa 2581 agaccttata catatacaac caaagatgta gccgtaaaaa ttgaacaaac acgttatctc 2641 gaagaagata agtttttagt ctttgtacca taaaagggaa ttaatatgaa aagtgaaaac 2701 aattctgtac caattgatca tgttataaaa gttgataacg aacgtcatat acgtgtttgg 2761 gaaactttcc ctaaaaatca atgtgataaa agaaataata ctattgttat tgcttctggt 2821 tttgctcgaa gaatggatca ttttgcaggt ttagctgaat atttatcaac caatggattt 2881 catgttattc gttatgattc acttaatcat gttggattaa gtagcggtga aattgatcag 2941 ttctcaatgt cagtaggtaa gaaaagttta ttaaccgtta ttgactggtt gaaatcagag 3001 catggtattg atcaggtagg tttaattgca tcgagtcttt ctgctcgaat tgcttatgat 3061 attgtcgctg atgttaattt gtctttttta attactgccg ttggtgtggt caatttacgg 3121 aatactcttg aacaagcgct taaatatgat tacttgcaga tggagataga tgaaatacca 3181 gaagatttag atttcgatgg atataattta ggttcaaagg tatttgttac agattgtttt 3241 gaaaataact gggatacatt agattcaact ataaataaaa cgaaaaattt aaatgtccca 3301 tttatagctt ttgtcgccaa tgatgatagt tgggtacaac agcacgaagt cgaagaatta 3361 atgagtaata tcaattcaga taaaaccaag atttactctt taataggttc atcacatgat 3421 ttaggtgaaa acctaattgt gttaagaaat ttctatcaat caattactaa agcagcaatt 3481 gcattagata gtaatttagt agggttagta agtgagatta ttgaaccaca atttgaagct 3541 ctcactattg ctacagtaaa tgaacgtcgt ttgaaaaata aaatacaaag taagtcatta 3601 gcttaattac aactgataca taaaccaaca aaaggaatat attatgaagt ttggaaatat 3661 ttgtttctca tatcagcctc caggtgagtc acataaagaa gtcatggatc gctttgttcg 3721 tttaggcgtt gcatcagaag aactaaattt tgatacttac tggactctcg agcatcattt 3781 tactgaattt ggactaacag gtaacctgtt tgttgcttgt gctaacttac ttggtcgaac 3841 caccaaactg aatgttggta ctatgggtat tgttcttcca acagctcacc ctgcacgtca 3901 gatggaagat ttattacttt tagatcaaat gtcaaaaggc cgttttaatt ttggtgttgt 3961 gcgtggcttg taccacaaag atttccgcgt ttttggtgta acgatggaag attctcgtgc 4021 tatcactgaa gattttcaca ccatgattat ggatggtaca aaaacaggta cacttcatac 4081 tgatggtaaa aacatcgagt tcccagatgt aaacgtttac ccagaggcgt atttagagaa 4141 aattccaaca tgcatgactg ctgaatcagc agtaacaacg acttggcttg ctgagcgtgg 4201 cttaccgatg gttcttagtt ggattattac aacgagtgaa aagaaagctc aaatggaact 4261 ctataatgct gttgctcgag atagcggtta cagtgaagag tacattaaaa acgttgatca 4321 cagtatgacc ctcatctgtt ctgtagatga agatggcaaa aaagctgaag atgtgtgccg 4381 tgagttttta ggtaattggt atgattcata cgtaaatgca accaatatct ttagtgaaag 4441 taaccaaact cgtggttatg attatcataa aggtcaatgg aaagattttg ttcttcaagg 4501 acatactaat actaaacgtc gtgttgatta tagcaacgat ctaaaccctg taggtacacc 4561 tgaaaaatgt attgaaatta ttcagcgtga tattgatgca acaggtatta ctaatattac 4621 ccttggtttc gaagcaaatg gctctgagga agaaatcatt gcctctatga aacgcttcat 4681 gacgcaagtt gcaccattct taaaagatcc aaaataaata aatcactcag attaacttta 4741 ataaataata taaggaatat aacatgaatt ttggattatt cttcctcaac tttcagcctg 4801 aaaatacatc gtcagaaaca gttttagata atatgatcaa tactgtctct ttagttgata 4861 aagattataa aaactttaca actgctttag tcaacgagca ccatttttct aaaaatggta 4921 ttgtcggtgc tccgatgaca gctgcaagct tcctattagg actaactgaa cgtttacata 4981 ttggttcttt aaatcaagta attacaacgc atcatccggt tcgtattgca gaagaagcaa 5041 gtttgcttga tcaaatgtca gacagccgct ttattctagg tctaagtgat tgtgttaatg 5101 attttgagat ggatttcttt aaacggcaac gtgactcaca gcagctacaa tttgaagctt 5161 gctatgacat cattaatgaa gctatcacaa ctaattactg ccaagctaat aatgattttt 5221 ataacttccc tcgtatctca attaatcctc attgcttaag taaagagaat atgaagcaat 5281 atattttggc ttctagtgtg agtgttgttg agtgggctgc taaaaaagcg cttccactaa 5341 catatcgttg gagcgatagc cttgaagata aagagattct ttataagcgt tatttagaag 5401 ttgcagcaaa gcataatatt gacgtttcta atgtcgagca tcagttcccg ctgcttgtaa 5461 atttaaatca tgatcgtgat gttgctcatc aagaagcaac ggcctattta gtaagttata 5521 ttgctgaagt atacccacat ctaaatcagc aacaaaaaat tgctgaactt attagccaac 5581 atgcgattgg tactgataat gattactatg aatcaacatt aaatgcgtta gagcgtacag 5641 gttcaaaaaa tgtattactt tcttttgaat caatgaagaa tcatgatgat gttgtaaaag 5701 tgattaatat ggttaatgag aagattcaaa agaatttacc aagctcgtaa gtgtaaagga 5761 agcggtgtta ttatgaataa atggaattac ggagtcttct tcgttaactt ttataataaa 5821 ggccaacaag agccatcaaa aacgatgaat aatgcattag aaacattacg tattattgat 5881 gaagatacat ctatttatga tgtgattaat attgatgacc actatcttgt aaagaaagac 5941 agtgaagata aaaagctagc gtcttttatt acactaggag aaaaactata tgtgcttgct 6001 accagtgaaa acacagttga tattgcagcg aaatatgcat taccgttagt tttcaaatgg 6061 gatgatataa atgaggaacg acttaaattg ttgagttttt ataatgcatc cgcaagtaaa 6121 tataacaaga atatagattt ggttcgacac cagcttatgt tacatgtcaa tgttaatgag 6181 gcagaaactg tagcaaaaga agaactcaaa ttatatattg aaaactatgt agcatgtaca 6241 cagcctagta attttaatgg ctcgattgat agtattattc agagtaacgt gacagggagt 6301 tataaagact gtttgtcata tgtagcgaat cttgctggta aatttgataa tactgtggac 6361 ttcttacttt gttttgagtc aatgcaagat caaaataaga aaaaatcagt aatgatagat 6421 cttaataatc aagttattaa gttccgccaa gataataatc taatctaatc tacaatcatt 6481 gcctcttata atggcagtgc taatttaaag ttctgccatt atatttaatt atattttaaa 6541 taggattaaa catgactatt acattagata cttgcgaaaa agatattatt gtaagtacag 6601 agatcgacga tattattttt acatcatcac ctcttgatat tacttacgat gaacaagaaa 6661 gaataaagca taaattaata ttagaatcat ttcgttacca ctataataat aatgaagatt 6721 ataagtcttt ctgtaatact cagggggtag acgaaaatat ttcatcactt gatgatatcc 6781 ctgtttttcc gacctcaatg tttaagtatg caaaaatatg tacagcagat gagtctaaca 6841 ttgaaaactg gttcacaagt agtggtacga gtggtgtaaa aagtcatatt gcccgtgatc 6901 gtgtaagtat tgaacgttta cttggttctg taaattatgg aatgaaatat cttggttcat 6961 ttcatgaaaa tcagctagaa cttgttaata tgggacctga tcgttttaat gctaaaaatg 7021 tttggtttaa gtatgtaatg agtcttgttg agttattata tccaactaca tttactgtaa 7081 ataacgatga aatagatttt gaacttacta ttaaaagttt aaaagaaatc tataataaag 7141 gaaaaggtat ttgtttaatt ggccctccgt atttcattta tttgctatgc cagtacatga 7201 aagagaatga tattgaattt aatgcaggta atcgcatctt tattattact ggcggtggtt 7261 ggaaaactaa gcaaaaacaa gcactaaatc gtcaagattt taatcaacta ttgatggaaa 7321 ccttccattt agcacatgaa agtcagattc gagatacatt taatcaagtt gaattaaata 7381 cgtgtttctt tgaagataac cgtcagcgta agcatgttcc gccatgggtt tatgcacgtg 7441 cacttgatcc tgtaactcta aagcctgttg aagatggtca agaagggctt attagttaca 7501 tggatgcatc atcaacgagt tatccaacat ttatcgttac tgacgatatc ggtataattc 7561 atacaattaa agatccagat ccgtaccaag gcactacgat tgatattgtc cgtcgtttga 7621 atacgagaga gcagaaaggg tgttcattat caatggcatc aggcttgaaa tagcttacaa 7681 ggaagatgta tgattttaaa ttgtaaaata attaaaattg aagcttctga atgtaatatt 7741 tttaaagtat ttattaagcc tgataagtgt ctcaatttta aagctggtca atatgtttta 7801 gcatatttag atggtaaaaa attacctttc tcaattgcta attgtccgac atgtaatgag 7861 cttatagagt tacatgttgg gagttcggta aaagaaaccg cagttaaatc tatttctcat 7921 tttttagatg cttttgtgaa tagctctgaa atacaaatag atgcacctca tggtaatgct 7981 tggctacgtg aagatagtaa ttctccatta ttacttatag ctggaggtac tgggttatca 8041 tatatcaata gtattcttag taattgtgta aataggaatt tgccccgttc tatttatgtt 8101 tactggggag ttaataatat tgatttatta tatgcagaca ctcaattaaa agctctttct 8161 agcgatttta acaatgttaa atacgtacct gttttggaaa actttgacaa taattggtac 8221 ggaaaaaaag gcaatgttat tgatgcaata atagaggact ttggtgattt atcagaattt 8281 gatatctatg tttgcggccc tcaaggtatg acacgtagtg ttcgtgaaaa gttaacatca 8341 cttaaaaaag ctgatactga taaaatgttt gccgatgctt ttgcatatat gtgatactag 8401 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 8461 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 8521 ctgcgtttat atactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag 8581 agatactgag cactactaga gaaagaggag aaatactaga tgcgtaaagg agaagaactt 8641 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt 8701 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt 8761 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt 8821 gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc 8881 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag 8941 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt 9001 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca 9061 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt 9121 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca 9181 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt 9241 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct 9301 gggattacac atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat 9361 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 9421 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //