LOCUS BBa_K771202 4158 bp DNA linear 23-Dec-2024 DEFINITION VioB with Membrane Anchor 3 ACCESSION BBa_K771202 VERSION BBa_K771202.1 FEATURES Location/Qualifiers RBS 5..13 /label=RBS misc_feature 22..24 /label=Start misc_feature 4156..4158 /label=stop misc_feature 1..1107 /label=BBa_K771003 CDS 1114..1161 /label=FL3 CDS 1162..4158 /label=VioB ORIGIN 1 aaaaataagg aggaaaaaaa aatgaaaaag atttggctgg cgctggctgg tttagtttta 61 gcgtttagcg catcggcggg cgtgaaggaa tctctggtgg ctgaggccgc cgcaaaagaa 121 gcagcagcta aggaagctgc ggcgaagatg accagtagct atctgcattt tccggagttt 181 gatccggtca ttttctcaat aggacccgtg gcgcttcact ggtacggcct gatgtatctg 241 gtgggtttca tttttgcaat gtggctggca acacgacggg cgaatcgtcc gggcagcggc 301 tggaccaaaa atgaagttga aaacttactc tatgcgggct tcctcggcgt cttcctcggg 361 ggacgtattg gttatgttct gttctacaat ttcccgcagt ttatggccga tccgctgtat 421 ctgttccgtg tctgggacgg cggcatgtct ttccacggcg gcctgattgg cgttatcgtg 481 gtgatgatta tcttcgcccg ccgtactaaa cgttccttct tccaggtctc tgattttatc 541 gcaccactca ttccgtttgg tcttggtgcc gggcgtctgg gcaactttat taacggtgaa 601 ttgtggggcc gcgttgaccc gaacttcccg tttgccatgc tgttccctgg ctcccgtaca 661 gaagatattt tgctgctgca aaccaacccg cagtggcaat ccattttcga cacttacggt 721 gtgctgccgc gccacccatc acagctttac gagctgctgc tggaaggtgt ggtgctgttt 781 attatcctca acctgtatat tcgtaaacca cgcccaatgg gagctgtctc aggtttgttc 841 ctgattggtt acggcgcgtt tcgcatcatt gttgagtttt tccgccagcc cgacgcgcag 901 tttaccggtg cctgggtgca gtacatcagc atggggcaaa ttctttccat cccgatgatt 961 gtcgcgggtg tgatcatgat ggtctgggca tatcgtcgca gcccacagca acacgtttcc 1021 actagagctg aggccgccgc aaaagaagca gcagctaagg aagctgcggc gaagccccct 1081 ccagcgctgc cgccaaaacg tcgccggact agagctgagg ccgccgcaaa agaagcagca 1141 gctaaggaag ctgcggcgaa gatgagcatt ctggatttcc cgcgtatcca cttccgtggc 1201 tgggcccgtg tcaatgcgcc gaccgcgaac cgcgatccgc acggccacat cgatatggcc 1261 agcaataccg tggcgatggc gggtgagccg ttcgacctgg cacgccatcc tacggagttc 1321 caccgtcacc tgcgctccct gggtccgcgc ttcggcttgg atggtcgtgc tgacccggaa 1381 ggcccgttca gcctggccga gggctacaac gctgccggta acaaccactt ttcgtgggag 1441 agcgcaaccg ttagccacgt gcaatgggat ggcggtgagg cggatcgtgg tgacggtctg 1501 gtcggtgctc gtttggcact gtggggtcac tacaatgatt atctgcgtac caccttcaat 1561 cgtgctcgtt gggtcgacag cgacccgacg cgccgtgacg ctgcacaaat ctatgcgggc 1621 caattcacca ttagcccggc tggtgccggt ccgggtacgc cgtggctgtt tacggcagac 1681 attgatgata gccatggtgc acgttggacg cgtggcggcc acattgcaga gcgtggcggc 1741 cacttcttgg atgaagagtt tggtctggca cgcctgtttc agttctctgt gccgaaagat 1801 cacccacatt ttctgtttca cccgggtccg tttgattccg aggcctggcg tcgtctgcaa 1861 ttggctctgg aggatgacga cgttctgggt ctgaccgtgc aatatgcgtt gttcaatatg 1921 agcaccccgc ctcagccgaa cagcccggtt tttcacgata tggtcggtgt tgtcggtctg 1981 tggcgtcgtg gtgaactggc gagctacccg gctggtcgtc tgctgcgtcc gcgtcaaccg 2041 ggtctgggtg acctgaccct gcgcgtcaac ggtggtcgcg ttgcgctgaa tttggcgtgt 2101 gccattccgt tcagcactcg tgccgcgcag ccaagcgcac cggaccgcct gaccccggac 2161 ctgggtgcca aactgccgct gggcgatctg ctgctgcgtg atgaggacgg cgcactgttg 2221 gcacgtgtgc cgcaggctct gtaccaagac tattggacga atcacggtat tgtggacctg 2281 ccgctgctgc gcgaaccgcg tggtagcttg accctgagca gcgaactggc ggagtggcgt 2341 gagcaagact gggtcaccca aagcgacgcg tctaacctgt acctggaggc accggatcgc 2401 cgtcacggtc gctttttccc tgagagcatc gcgctgcgca gctactttcg cggtgaagcg 2461 cgtgcgcgtc cggatatccc gcatcgtatc gagggcatgg gcctggtcgg cgtcgaatct 2521 cgtcaggatg gcgacgctgc ggaatggcgt ctgacgggtc tgcgtccggg tccggcacgc 2581 attgttctgg acgatggtgc cgaggcgatc cctctgcgtg ttctgcctga cgattgggcg 2641 ctggatgacg cgaccgtcga agaagtggat tacgcctttt tgtaccgcca cgttatggcg 2701 tattacgagc tggtgtatcc attcatgagc gacaaggtgt tttccctggc tgatcgttgc 2761 aaatgtgaaa cgtacgcacg tctgatgtgg cagatgtgtg atccgcagaa ccgcaacaag 2821 tcctattaca tgccgagcac ccgcgaactg tcggcaccga aagctcgttt gttcttgaag 2881 tatctggccc acgtggaagg ccaggcacgc ctgcaagcac ctccgccagc gggtccggca 2941 cgcattgaat ctaaagccca gttggcggca gagctgcgta aagccgtcga cctggagctg 3001 tctgtgatgc tgcaatacct gtacgcggcg tatagcattc cgaactatgc acagggccaa 3061 caacgtgttc gtgacggtgc gtggaccgcc gagcagctgc aactggcgtg cggtagcggt 3121 gaccgtcgcc gtgatggcgg tattcgtgca gcactgctgg aaattgctca tgaagaaatg 3181 attcattacc tggtcgttaa caacctgctg atggccctgg gcgagccgtt ctacgcgggt 3241 gtcccgctga tgggcgaagc ggcacgtcag gcgtttggcc tggacaccga gttcgctctg 3301 gaaccgttta gcgaaagcac gctggcacgt tttgttcgtc tggaatggcc gcactttatc 3361 ccagcaccgg gcaaatccat cgcggactgc tatgccgcca ttcgtcaggc gtttttggat 3421 ctgccggact tgtttggtgg cgaggcaggt aagcgtggcg gtgaacacca cctgttcctg 3481 aatgagctga ccaaccgtgc gcatccgggt tatcaactgg aagttttcga tcgcgactcg 3541 gcgctgtttg gtattgcatt tgtgaccgat cagggcgaag gtggcgctct ggacagcccg 3601 cactacgaac atagccattt tcaacgtctg cgtgaaatga gcgcgcgtat catggctcaa 3661 agcgcaccgt tcgaaccggc gctgccggcg ttgcgtaatc cggttctgga tgagagcccg 3721 ggttgccaac gtgtcgcaga cggtcgtgcg cgtgcgctga tggcattgta ccaaggcgtt 3781 tatgagctga tgtttgcgat gatggcgcag cacttcgccg tgaaaccgct gggtagcttg 3841 cgtcgcagcc gcctgatgaa cgcagcaatc gatctgatga ccggtctgtt gcgtccgctg 3901 agctgcgcgc tgatgaacct gccaagcggc atcgccggtc gcacggccgg tccgccgctg 3961 ccgggtccgg ttgacacccg tagctatgac gactacgcgc tgggctgtcg catgctggca 4021 cgccgttgcg agcgtctgct ggagcaggcg agcatgctgg aaccgggttg gctgccggat 4081 gcgcagatgg agctgctgga tttctatcgt cgccaaatgc tggacttggc gtgcggcaaa 4141 ctgagccgcg aggcctaa //