BBa_K772001 1 BBa_K772001 EpCAM Binding Dock: C-terminus 2012-09-23T11:00:00Z 2015-05-08T01:13:15Z CD225 (EpCAM antibody) is commonly used for the test of presence of EpCAM in the laboratories. The sequences of OmpA and TEV protease terminuses are acquired from the Registry. This gene sequence includes an EpCAM (Epithelial Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease C terminus. It is planned to use this subpart with BBa_772, which contains only TEV protease N terminus differently instead of C terminus, in order to induce a signal reaction in the case of EpCAM presence. EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes. On the upstream region of protein sequence, there are a constitutive pLac promoter and appropiate RBS. To see how this part works: Experience. false false _1024_ 0 9573 9 Not in stock true There are two protein sequences between domains and the dock: One between the binding domain (antibody) and dock and one between the part of TEV protease. false Mustafa Elitok annotation2203669 1 pCons Promoter range2203669 1 1 72 annotation2203672 1 TEV Protease C terminus range2203672 1 94 419 annotation2203671 1 OmpA (Dock) range2203671 1 420 857 annotation2203670 1 RBS range2203670 1 73 93 annotation2203673 1 CD215 Antibody range2203673 1 858 1159 annotation2203674 1 Binding Dock range2203674 1 94 1159 BBa_K772001_sequence 1 ctagagttgacggctagctcagtcctaggtacagtgctagcaaagaggagaaatactagatggactacaaagacgacgacgacaaaggtggaggaggttctggaggcggtggaagtggtggcggaggtagcaagagcatgtctagcatggtgtcagacaccagttgcacattcccttcatctgatggcatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaatgctgactcagtattgtgggggggccataaagttttcatgagcaaacctgaagagccttttcagccagttaaggaagcgactcaactcatgagtgaattggtgtactcgcaataaggtggaggaggttctggaggcggtggaagtggtggcggaggtagcaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcggtggaggaggttctggaggcggtggaagtggtggcggaggtagccaggttaaattacaacagagcggtgcagaactggttcgtccgggtgcaagcgttaaactgagctgtaaagcaagcggttatacctttaccaattattggattaattgggttaaacagcgtccgggtcagggtctggaatggattggtaatatttatccgagctatatttataccaattataatcaggaatttaaagataaagttaccctgaccgttgatgaaagcagcagcaccgcatatatgcagctgagcagcccgaccagcgaagatagcgcagtttattattgtacccgtagcccgtatggttatgatgaatatggtctggattattggggtcagggtaccaccgttaccgttagcagctaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z