LOCUS       BBa_K772009             1745 bp    DNA     linear       22-May-2024
DEFINITION  GFP - LasR protein complex with flag tags
ACCESSION   BBa_K772009
VERSION     BBa_K772009.1  
FEATURES             Location/Qualifiers
     RBS             1..20
                     /label=RBS
     misc_feature    21..46
                     /label=Flag tag
     misc_feature    47..767
                     /label=GFP
     CDS             768..857
                     /label=TEV cleavage site
     misc_feature    858..1614
                     /label=LasR
     misc_feature    1615..1745
                     /label=Double terminator
     CDS             21..1614
                     /label=Anchor-inducer + flag
ORIGIN
        1 ctagagaaag aggagaaata ctagagacta caaagacgac gacgacaaaa tgcgtaaagg
       61 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      121 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      181 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      241 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
      301 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
      361 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
      421 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
      481 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa
      541 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
      601 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca
      661 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt
      721 aacagctgct gggattacac atggcatgga tgaactatac aaataataag ccgcggatca
      781 agagcaggtg gaggcggagg cggcggaggt ggaggtgaaa atctttattt tcaaggaggc
      841 aaactgggag gcggcggaat ggccttggtt gacggttttc ttgagctgga acgctcaagt
      901 ggaaaattgg agtggagcgc catcctccag aagatggcga gcgaccttgg attctcgaag
      961 atcctgttcg gcctgttgcc taaggacagc caggactacg agaacgcctt catcgtcggc
     1021 aactacccgg ccgcctggcg cgagcattac gaccgggctg gctacgcgcg ggtcgacccg
     1081 acggtcagtc actgtaccca gagcgtactg ccgattttct gggaaccgtc catctaccag
     1141 acgcgaaagc agcacgagtt cttcgaggaa gcctcggccg ccggcctggt gtatgggctg
     1201 accatgccgc tgcatggtgc tcgcggcgaa ctcggcgcgc tgagcctcag cgtggaagcg
     1261 gaaaaccggg ccgaggccaa ccgtttcata gagtcggtcc tgccgaccct gtggatgctc
     1321 aaggactacg cactgcaaag cggtgccgga ctggccttcg aacatccggt cagcaaaccg
     1381 gtggttctga ccagccggga gaaggaagtg ttgcagtggt gcgccatcgg caagaccagt
     1441 tgggagatat cggttatctg caactgctcg gaagccaatg tgaacttcca tatgggaaat
     1501 attcggcgga agttcggtgt gacctcccgc cgcgtagcgg ccattatggc cgttaatttg
     1561 ggtcttatta ctctcgctgc aaacgacgaa aactacgctt tagtagctta ataaccaggc
     1621 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1681 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1741 atata
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