BBa_K772100 1 BBa_K772100 EpCAM Detection Device 2012-09-25T11:00:00Z 2015-05-08T01:13:15Z CD225 (EpCAM antibody) is commonly used for the test of presence of EpCAM in the laboratories. The sequences of OmpA and TEV protease terminuses are acquired from the Registry. This gene sequence includes both of EpCAM Binding Docks; BBa_K772001 and BBa_K772002. Thus, this composite part is the main component of CTC (Circulating Tumor Cell) detection system. Docks include an EpCAM (Epithelial Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease C or N terminus. Hopefully, after binding to the EpCAM antigen, TEV protease domains will fix together to form the full structure in order to activate the signalling pathway. (For more information: BBa_K772003) EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes. On the upstream region of protein sequences, there are a constitutive pLac promoter and appropriate RBS. To see how this part works: Experience. false false _1024_ 0 9573 9 It's complicated true There are two protein sequences between domains and the dock: One between the binding domain (antibody) and dock and one between the domain of TEV protease. false Mustafa Elitok component2211341 1 BBa_K772001 component2211348 1 BBa_K772002 annotation2211341 1 BBa_K772001 range2211341 1 1 1159 annotation2211348 1 BBa_K772002 range2211348 1 1168 2305 BBa_K772002 1 BBa_K772002 EpCAM Binding Dock: N terminus 2012-09-23T11:00:00Z 2015-05-08T01:13:15Z CD225 (EpCAM antibody) is commonly used for the test of presence of EpCAM in the laboratories. The sequences of OmpA and TEV protease terminuses are acquired from the Registry. This gene sequence includes an EpCAM (Epithelial p? Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease N terminus. It is planned to use this subpart with BBa_772, which contains only TEV protease C terminus differently instead of N terminus, in order to induce a signal reaction in the case of EpCAM presence. EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes. On the upstream region of protein sequence, there are a constitutive pLac promoter and appropiate RBS. To see how this part works: Experience. false false _1024_ 0 9573 9 Not in stock true There are two protein sequences between domains and the dock: One between the binding domain (antibody) and dock and one between the part of TEV protease. false Mustafa Elitok annotation2203682 1 OmpA (Dock) range2203682 1 399 836 annotation2203686 1 CD215 antibody range2203686 1 837 1138 annotation2203683 1 RBS range2203683 1 73 93 annotation2203681 1 pCons Promoter range2203681 1 1 72 annotation2203684 1 Binding Dock range2203684 1 94 1138 annotation2203685 1 TEV Protease N terminus range2203685 1 94 398 BBa_K772001 1 BBa_K772001 EpCAM Binding Dock: C-terminus 2012-09-23T11:00:00Z 2015-05-08T01:13:15Z CD225 (EpCAM antibody) is commonly used for the test of presence of EpCAM in the laboratories. The sequences of OmpA and TEV protease terminuses are acquired from the Registry. This gene sequence includes an EpCAM (Epithelial Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease C terminus. It is planned to use this subpart with BBa_772, which contains only TEV protease N terminus differently instead of C terminus, in order to induce a signal reaction in the case of EpCAM presence. EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes. On the upstream region of protein sequence, there are a constitutive pLac promoter and appropiate RBS. To see how this part works: Experience. false false _1024_ 0 9573 9 Not in stock true There are two protein sequences between domains and the dock: One between the binding domain (antibody) and dock and one between the part of TEV protease. false Mustafa Elitok annotation2203671 1 OmpA (Dock) range2203671 1 420 857 annotation2203670 1 RBS range2203670 1 73 93 annotation2203669 1 pCons Promoter range2203669 1 1 72 annotation2203673 1 CD215 Antibody range2203673 1 858 1159 annotation2203672 1 TEV Protease C terminus range2203672 1 94 419 annotation2203674 1 Binding Dock range2203674 1 94 1159 BBa_K772001_sequence 1 ctagagttgacggctagctcagtcctaggtacagtgctagcaaagaggagaaatactagatggactacaaagacgacgacgacaaaggtggaggaggttctggaggcggtggaagtggtggcggaggtagcaagagcatgtctagcatggtgtcagacaccagttgcacattcccttcatctgatggcatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaatgctgactcagtattgtgggggggccataaagttttcatgagcaaacctgaagagccttttcagccagttaaggaagcgactcaactcatgagtgaattggtgtactcgcaataaggtggaggaggttctggaggcggtggaagtggtggcggaggtagcaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcggtggaggaggttctggaggcggtggaagtggtggcggaggtagccaggttaaattacaacagagcggtgcagaactggttcgtccgggtgcaagcgttaaactgagctgtaaagcaagcggttatacctttaccaattattggattaattgggttaaacagcgtccgggtcagggtctggaatggattggtaatatttatccgagctatatttataccaattataatcaggaatttaaagataaagttaccctgaccgttgatgaaagcagcagcaccgcatatatgcagctgagcagcccgaccagcgaagatagcgcagtttattattgtacccgtagcccgtatggttatgatgaatatggtctggattattggggtcagggtaccaccgttaccgttagcagctaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata BBa_K772100_sequence 1 ctagagttgacggctagctcagtcctaggtacagtgctagcaaagaggagaaatactagatggactacaaagacgacgacgacaaaggtggaggaggttctggaggcggtggaagtggtggcggaggtagcaagagcatgtctagcatggtgtcagacaccagttgcacattcccttcatctgatggcatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaatgctgactcagtattgtgggggggccataaagttttcatgagcaaacctgaagagccttttcagccagttaaggaagcgactcaactcatgagtgaattggtgtactcgcaataaggtggaggaggttctggaggcggtggaagtggtggcggaggtagcaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcggtggaggaggttctggaggcggtggaagtggtggcggaggtagccaggttaaattacaacagagcggtgcagaactggttcgtccgggtgcaagcgttaaactgagctgtaaagcaagcggttatacctttaccaattattggattaattgggttaaacagcgtccgggtcagggtctggaatggattggtaatatttatccgagctatatttataccaattataatcaggaatttaaagataaagttaccctgaccgttgatgaaagcagcagcaccgcatatatgcagctgagcagcccgaccagcgaagatagcgcagtttattattgtacccgtagcccgtatggttatgatgaatatggtctggattattggggtcagggtaccaccgttaccgttagcagctaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatatactagagctagagttgacggctagctcagtcctaggtacagtgctagcaaagaggagaaatactagatggactacaaagacgacgacgacaaaggtggaggaggttctggaggcggtggaagtggtggcggaggtagcggagaaagcttgtttaagggaccacgtgattacaacccgatatcgagcaccatttgtcacttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttagaagaaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaagaacaccacgactttgcaacaacacctcattgatgggagggacatgataattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaaactggtggaggaggttctggaggcggtggaagtggtggcggaggtagcaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcggtggaggaggttctggaggcggtggaagtggtggcggaggtagccaggttaaattacaacagagcggtgcagaactggttcgtccgggtgcaagcgttaaactgagctgtaaagcaagcggttatacctttaccaattattggattaattgggttaaacagcgtccgggtcagggtctggaatggattggtaatatttatccgagctatatttataccaattataatcaggaatttaaagataaagttaccctgaccgttgatgaaagcagcagcaccgcatatatgcagctgagcagcccgaccagcgaagatagcgcagtttattattgtacccgtagcccgtatggttatgatgaatatggtctggattattggggtcagggtaccaccgttaccgttagcagctaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata BBa_K772002_sequence 1 ctagagttgacggctagctcagtcctaggtacagtgctagcaaagaggagaaatactagatggactacaaagacgacgacgacaaaggtggaggaggttctggaggcggtggaagtggtggcggaggtagcggagaaagcttgtttaagggaccacgtgattacaacccgatatcgagcaccatttgtcacttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttagaagaaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaagaacaccacgactttgcaacaacacctcattgatgggagggacatgataattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaaactggtggaggaggttctggaggcggtggaagtggtggcggaggtagcaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcggtggaggaggttctggaggcggtggaagtggtggcggaggtagccaggttaaattacaacagagcggtgcagaactggttcgtccgggtgcaagcgttaaactgagctgtaaagcaagcggttatacctttaccaattattggattaattgggttaaacagcgtccgggtcagggtctggaatggattggtaatatttatccgagctatatttataccaattataatcaggaatttaaagataaagttaccctgaccgttgatgaaagcagcagcaccgcatatatgcagctgagcagcccgaccagcgaagatagcgcagtttattattgtacccgtagcccgtatggttatgatgaatatggtctggattattggggtcagggtaccaccgttaccgttagcagctaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z