BBa_K124014 1 BBa_K124014 Bacteriophage Holin Gene pS105 2008-10-08T11:00:00Z 2015-05-08T01:09:44Z This part was graciously donated to the Brown iGEM Team by Ry Young at Texas A&M. The Cell Lysis Cassette consists of an S, R, and Rz protein. When paired with an activator, the cassette is expressed and cell lysis is induced. The S105 strain of the cassette has the initial 6 base pairs at the beginning of the wild type sequence removed. This causes cell lysis to be induced at a faster rate than the wild type. false false _221_ 0 3367 9 It's complicated true No design considerations. true Katherine Jacobs BBa_K112806 1 BBa_K112806 [T4 endolysin] 2008-10-31T12:00:00Z 2015-05-08T01:09:19Z Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts Released HQ 2013 The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer. false true _224_ 0 3025 171 In stock false High expression of lysozyme only is toxic to bacteria. false Jin Huh annotation1999018 1 T4 Endolysin range1999018 1 17 511 BBa_K772101 1 BBa_K772101 Self-Destruction Kit: Endolysin + Holin 2012-09-25T11:00:00Z 2015-05-08T01:13:15Z Gene sequences of endolysin and holin are gathered from the Registry. Endolysin is a lysozyme which cleaves the glycosidic bond between the C-1 of N-acetylmuramic acid (NAM) and the C-4 of N-acetylglucosamine (NAG) in the bacterial peptidoglycan (PG). This leads to the complete lysis of the cell. Holin is a protein that assembles together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. It is sourced from T4 bacteriophage. This composite part can be used to induce self-destruction with a regulatory device in the upstream region. false false _1024_ 0 9573 9 Not in stock false There is no RBS in the upstream region, so during the assemble procedure, this must be taken care of. false Mustafa Elitok component2202512 1 BBa_K124014 component2202511 1 BBa_K112806 annotation2202512 1 BBa_K124014 range2202512 1 523 839 annotation2202511 1 BBa_K112806 range2202511 1 1 514 BBa_K772101_sequence 1 atacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagctactagaggctgctaaaaaagccggagtagaagatggtagaaatcaataagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttctataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagtatgccagaaaaacatgacctgttggccgccattctcgcggcaaaggaacaaggcatcggggcaatccttgcgtttgcaatggcgtaccttcgcggcaga BBa_K124014_sequence 1 gctgctaaaaaagccggagtagaagatggtagaaatcaataagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttctataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagtatgccagaaaaacatgacctgttggccgccattctcgcggcaaaggaacaaggcatcggggcaatccttgcgtttgcaatggcgtaccttcgcggcaga BBa_K112806_sequence 1 atacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z