LOCUS       BBa_K772103             1269 bp    DNA     linear       22-Nov-2024
DEFINITION  GFP - LacZ protein complex generator
ACCESSION   BBa_K772103
VERSION     BBa_K772103.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..82
                     /label=RBS
     misc_feature    83..803
                     /label=GFP
     CDS             804..893
                     /label=TEV cleavage site
     misc_feature    894..1137
                     /label=LacZ
     misc_feature    1138..1269
                     /label=Double Terminator
     CDS             83..1137
                     /label=Anchor - Reporter
     misc_feature    63..1269
                     /label=BBa_K772006
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agctagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc actggagttg
      121 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
      181 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
      241 aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg
      301 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt
      361 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag
      421 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag
      481 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca
      541 tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg
      601 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
      661 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca
      721 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
      781 gcatggatga actatacaaa taataagccg cggatcaaga gcaggtggag gcggaggcgg
      841 cggaggtgga ggtgaaaatc tttattttca aggaggcaaa ctgggaggcg gcggagagga
      901 aacagctatg accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga
      961 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     1021 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     1081 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgacc
     1141 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1201 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1261 gtttatata
//