LOCUS       BBa_K772104             1379 bp    DNA     linear       23-May-2024
DEFINITION  pLac IPTG inducible generator
ACCESSION   BBa_K772104
VERSION     BBa_K772104.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    1316..1354
                     /label=LVA
     CDS             227..1354
                     /label=lacI-LVA
     misc_feature    227..1354
                     /label=BBa_C0012
     misc_feature    1355..1379
                     /label=Help:Barcodes
     CDS             227..1354
                     /label=BBa_C0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg tgaatgtgaa
      241 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg
      301 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat
      361 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt
      421 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc
      481 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag
      541 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct
      601 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa
      661 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc
      721 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat
      781 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca
      841 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc
      901 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat
      961 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct
     1021 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta
     1081 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga
     1141 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc
     1201 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt
     1261 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc
     1321 aaacgacgaa aactacgctt tagtagctta ataactctga tagtgctagt gtagatctc
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