BBa_K774000 1 B-M Bacterial-Mammalian Promoter: BBaK216005 + BHN + CArG promoter sequence E9-ns2 2012-06-24T11:00:00Z 2015-05-08T01:13:15Z PyeaR promoter (BBa_K216005) and the E9-ns2 CArG promoter (Ref:1) Ref1: Scott, S.D., Joiner, M.C. & Marples, B., 2002. Optimizing radiation-responsive gene promoters for radiogenetic cancer therapy. Gene therapy, 9(20), p.1396-402. Available at: http://www.ncbi.nlm.nih.gov/pubmed/12365005 Released HQ 2013 A hybrid of the PyeaR promoter (BBa_K216005) and the E9-ns2 CArG promoter (Ref:1). Two versions will be synthesised, with the promoters altering their position in relation to the 5???-end of the sequence. false false _1026_ 0 11706 9 In stock true To provide additional restriction enzyme sites that may become useful during later cloning steps, BamHI, HindIII and NdeI have been added between the 2 promoters. It is envisaged that any open reading frame (e.g. RFP or GFP) will be cloned ???downstream??? (i.e. at the 3???-end) of these promoter sequences. false NRP-UEA-Norwich annotation2177195 1 BBaK216005 range2177195 1 1 100 annotation2177197 1 CArG promoter sequence E9-ns2 range2177197 1 120 208 annotation2177196 1 BHN range2177196 1 101 119 BBa_K774000_sequence 1 ttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaattatcaggcgtaccctgaaacgggatccaagcttcatatgccatataaggccatataaggccatataaggccatataaggccatataaggccatataaggccatataaggccatataaggccatataagg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z