LOCUS       BBa_K774004             1094 bp    DNA     linear       13-May-2024
DEFINITION  Bacterial-Mammalian promoter with eCFP reporter: BBaK216005 + CArG 
            promoter sequence + BBa_E0420
ACCESSION   BBa_K774004
VERSION     BBa_K774004.1  
FEATURES             Location/Qualifiers
     promoter        1..100
                     /label=BBaK216005
     misc_feature    101..119
                     /label=BHN
     promoter        120..208
                     /label=CArG promoter sequence E9-ns2
     promoter        1..208
                     /label=BBa_K774000
     misc_feature    221..224
                     /label=conserved
     RBS             217..228
                     /label=BBa_B0034
     CDS             235..957
                     /label=BBa_E0020
     stem_loop       977..1020
                     /label=stem_loop
     misc_feature    966..1045
                     /label=BBa_B0010
     terminator      966..1045
                     /label=BBa_B0010
     stem_loop       1061..1080
                     /label=T7 TE
     misc_feature    1087..1087
                     /label=stop
     polyA_site      1081..1094
                     /label=polya
     misc_feature    1054..1094
                     /label=BBa_B0012
     terminator      1054..1094
                     /label=BBa_B0012
     misc_feature    217..1094
                     /label=BBa_E0420
ORIGIN
        1 ttcccatcta taatcctccc tgattcttcg ctgatatggt gctaaaaagt aaccaataaa
       61 tggtatttaa aatgcaaatt atcaggcgta ccctgaaacg ggatccaagc ttcatatgcc
      121 atataaggcc atataaggcc atataaggcc atataaggcc atataaggcc atataaggcc
      181 atataaggcc atataaggcc atataaggta ctagagaaag aggagaaata ctagatggtg
      241 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      301 gtgaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      361 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      421 accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      481 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      541 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      601 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      661 gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc
      721 aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
      781 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      841 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
      901 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaataatac
      961 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1021 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1081 tttctgcgtt tata
//