LOCUS       BBa_K774005              990 bp    DNA     linear       22-Dec-2024
DEFINITION  Bacterial-Mammalian promoter with RFP reporter: BBaK216005 + BHN + 
            CArG promoter sequence E9-ns2 + B
ACCESSION   BBa_K774005
VERSION     BBa_K774005.1  
FEATURES             Location/Qualifiers
     promoter        1..100
                     /label=BBaK216005
     misc_feature    101..119
                     /label=BHN
     promoter        120..208
                     /label=CArG promoter sequence E9-ns2
     promoter        1..208
                     /label=BBa_K774000
     RBS             217..231
                     /label=RBS-1\Strong
     RBS             224..228
                     /label=RBS
     misc_feature    217..231
                     /label=BBa_B0030
     RBS             217..231
                     /label=BBa_B0030
     CDS             238..912
                     /label=mrfp1
     misc_feature    919..943
                     /label=Help:Barcodes
     CDS             238..943
                     /label=BBa_E1010
     misc_feature    952..990
                     /label=B1006
     stem_loop       961..982
                     /label=modified thr terminator
     polyA_site      983..990
                     /label=PolyA
     polyA_site      952..960
                     /label=PolyA
     terminator      952..990
                     /label=BBa_B1006
     misc_feature    217..990
                     /label=BBa_K081014
ORIGIN
        1 ttcccatcta taatcctccc tgattcttcg ctgatatggt gctaaaaagt aaccaataaa
       61 tggtatttaa aatgcaaatt atcaggcgta ccctgaaacg ggatccaagc ttcatatgcc
      121 atataaggcc atataaggcc atataaggcc atataaggcc atataaggcc atataaggcc
      181 atataaggcc atataaggcc atataaggta ctagagatta aagaggagaa atactagatg
      241 gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc
      301 gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc
      361 cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg
      421 tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac
      481 tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac
      541 ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa
      601 gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg
      661 ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc
      721 aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac
      781 atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc
      841 acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac
      901 tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgctactaga gaaaaaaaaa
      961 ccccgcccct gacagggcgg ggtttttttt
//