##gff-version 3
##sequence-region BBa_K774005 1 990
BBa_K774005	.	promoter	1	208	.	+	0	ID=BBa_K774000;Name=BBa_K774000
BBa_K774005	.	sequence_feature	101	119	.	+	0	ID=annotation2177196;Name=BHN;Parent=BBa_K774000
BBa_K774005	.	promoter	120	208	.	+	0	ID=annotation2177197;Name=CArG promoter sequence E9-ns2;Parent=BBa_K774000
BBa_K774005	.	promoter	1	100	.	+	0	ID=annotation2177195;Name=BBaK216005;Parent=BBa_K774000
BBa_K774005	.	engineered_region	217	990	.	+	0	ID=BBa_K081014;Name=BBa_K081014
BBa_K774005	.	ribosome_entry_site	217	231	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081014
BBa_K774005	.	ribosome_entry_site	217	231	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K774005	.	engineered_region	217	231	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K774005	.	ribosome_entry_site	224	228	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K774005	.	terminator	952	990	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081014
BBa_K774005	.	polyA_site	952	960	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K774005	.	polyA_site	983	990	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K774005	.	engineered_region	952	990	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K774005	.	stem_loop	961	982	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K774005	.	CDS	238	943	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K081014
BBa_K774005	.	engineered_tag	919	943	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K774005	.	CDS	238	912	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
>BBa_K774005
ttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaat
tatcaggcgtaccctgaaacgggatccaagcttcatatgccatataaggccatataaggccatataaggccatataagg
ccatataaggccatataaggccatataaggccatataaggccatataaggtactagagattaaagaggagaaatactag
atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagt
tcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtcc
gctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatc
ccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtta
ccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccga
cggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaa
ggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaa
aaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgt
tgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctacta
gagaaaaaaaaaccccgcccctgacagggcggggtttttttt