LOCUS       BBa_K774007              990 bp    DNA     linear       26-Dec-2024
DEFINITION  Mammalian-Bacterial promoter with RFP reporter: CArG promoter 
            sequence + BBaK216005 + BBa_K08101
ACCESSION   BBa_K774007
VERSION     BBa_K774007.1  
FEATURES             Location/Qualifiers
     promoter        1..90
                     /label=E9-ns2 CArG promoter
     misc_feature    91..109
                     /label=BHN
     promoter        110..208
                     /label=BBaK216005
     promoter        1..208
                     /label=BBa_K774001
     RBS             217..231
                     /label=RBS-1\Strong
     RBS             224..228
                     /label=RBS
     misc_feature    217..231
                     /label=BBa_B0030
     RBS             217..231
                     /label=BBa_B0030
     CDS             238..912
                     /label=mrfp1
     misc_feature    919..943
                     /label=Help:Barcodes
     CDS             238..943
                     /label=BBa_E1010
     misc_feature    952..990
                     /label=B1006
     stem_loop       961..982
                     /label=modified thr terminator
     polyA_site      983..990
                     /label=PolyA
     polyA_site      952..960
                     /label=PolyA
     terminator      952..990
                     /label=BBa_B1006
     misc_feature    217..990
                     /label=BBa_K081014
ORIGIN
        1 ccatataagg ccatataagg ccatataagg ccatataagg ccatataagg ccatataagg
       61 ccatataagg ccatataagg ccatataagg ggatccaagc ttcatatgtt cccatctata
      121 atcctccctg attcttcgct gatatggtgc taaaaagtaa ccaataaatg gtatttaaaa
      181 tgcaaattat caggcgtacc ctgaaacgta ctagagatta aagaggagaa atactagatg
      241 gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc
      301 gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc
      361 cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg
      421 tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac
      481 tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac
      541 ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa
      601 gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg
      661 ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc
      721 aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac
      781 atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc
      841 acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac
      901 tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgctactaga gaaaaaaaaa
      961 ccccgcccct gacagggcgg ggtttttttt
//