LOCUS       BBa_K774100              972 bp    DNA     linear       22-Nov-2024
DEFINITION  NIR A promoter, GFP fusion
ACCESSION   BBa_K774100
VERSION     BBa_K774100.1  
FEATURES             Location/Qualifiers
     promoter        1..88
                     /label=nir promoter
     misc_feature    73..78
                     /label=-10 sequence
     misc_feature    5..20
                     /label=Ntc-B binding motif
     misc_feature    36..44
                     /label=Ntc-A binding motif
     promoter        1..88
                     /label=BBa_K125100
     RBS             97..109
                     /label=RBS-3\Weak
     RBS             103..106
                     /label=RBS
     misc_feature    97..109
                     /label=BBa_B0032
     RBS             97..109
                     /label=BBa_B0032
     CDS             116..835
                     /label=GFP protein
     CDS             116..835
                     /label=BBa_E0040
     stem_loop       855..898
                     /label=stem_loop
     misc_feature    844..923
                     /label=BBa_B0010
     terminator      844..923
                     /label=BBa_B0010
     stem_loop       939..958
                     /label=T7 TE
     misc_feature    965..965
                     /label=stop
     polyA_site      959..972
                     /label=polya
     misc_feature    932..972
                     /label=BBa_B0012
     terminator      932..972
                     /label=BBa_B0012
     misc_feature    97..972
                     /label=BBa_E0240
ORIGIN
        1 gctaaatgcg taaactgcat atgccttcgc tgagtgtaat ttacgttaca aattttaacg
       61 aaacgggaac cctatattga tctctactta ctagagtcac acaggaaagt actagatgcg
      121 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
      181 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
      241 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
      301 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga
      361 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
      421 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
      481 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
      541 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
      601 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
      661 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
      721 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
      781 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta
      841 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      901 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      961 tctgcgttta ta
//