##gff-version 3
##sequence-region BBa_K774100 1 972
BBa_K774100	.	promoter	1	88	.	+	0	ID=BBa_K125100;Name=BBa_K125100
BBa_K774100	.	non_covalent_binding_site	36	44	.	+	0	ID=annotation1968489;Name=Ntc-A binding motif;Parent=BBa_K125100
BBa_K774100	.	sequence_feature	73	78	.	+	0	ID=annotation1968487;Name=-10 sequence;Parent=BBa_K125100
BBa_K774100	.	non_covalent_binding_site	5	20	.	+	0	ID=annotation1968488;Name=Ntc-B binding motif;Parent=BBa_K125100
BBa_K774100	.	promoter	1	88	.	+	0	ID=annotation1968486;Name=nir promoter;Parent=BBa_K125100
BBa_K774100	.	engineered_region	97	972	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_K774100	.	terminator	932	972	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K774100	.	polyA_site	959	972	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K774100	.	stem_loop	939	958	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K774100	.	stop_codon	965	965	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K774100	.	engineered_region	932	972	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K774100	.	ribosome_entry_site	97	109	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K774100	.	ribosome_entry_site	97	109	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K774100	.	ribosome_entry_site	103	106	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K774100	.	engineered_region	97	109	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K774100	.	terminator	844	923	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K774100	.	stem_loop	855	898	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K774100	.	engineered_region	844	923	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K774100	.	CDS	116	835	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K774100	.	CDS	116	835	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_K774100
gctaaatgcgtaaactgcatatgccttcgctgagtgtaatttacgttacaaattttaacgaaacgggaaccctatattg
atctctacttactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattc
ttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaa
acttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggt
gttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtac
aggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgt
taatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataac
tcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaag
atggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaacca
ttacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaaca
gctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttata