LOCUS BBa_K774102 2903 bp DNA linear 29-Nov-2024 DEFINITION Multi sensor - for calculation of specific concentrations of nitrates nitrites and nitric oxide usin ACCESSION BBa_K774102 VERSION BBa_K774102.1 FEATURES Location/Qualifiers promoter 1..88 /label=nir promoter misc_feature 73..78 /label=-10 sequence misc_feature 5..20 /label=Ntc-B binding motif misc_feature 36..44 /label=Ntc-A binding motif promoter 1..88 /label=BBa_K125100 CDS 95..814 /label=GFP protein CDS 95..814 /label=BBa_E0040 promoter 823..912 /label=E9-ns2 CArG promoter misc_feature 913..931 /label=BHN promoter 932..1030 /label=BBaK216005 promoter 823..1030 /label=BBa_K774001 misc_feature 1043..1046 /label=conserved RBS 1039..1050 /label=BBa_B0034 CDS 1057..1779 /label=BBa_E0020 stem_loop 1799..1842 /label=stem_loop misc_feature 1788..1867 /label=BBa_B0010 terminator 1788..1867 /label=BBa_B0010 stem_loop 1883..1902 /label=T7 TE misc_feature 1909..1909 /label=stop polyA_site 1903..1916 /label=polya misc_feature 1876..1916 /label=BBa_B0012 terminator 1876..1916 /label=BBa_B0012 misc_feature 1039..1916 /label=BBa_E0420 misc_feature 823..1916 /label=BBa_K774006 promoter 1925..2068 /label=narGp promoter 1925..2068 /label=BBa_M33001 CDS 2095..2769 /label=mrfp1 misc_feature 2776..2800 /label=Help:Barcodes CDS 2095..2775 /label=BBa_E1010 misc_feature 2081..2084 /label=conserved RBS 2077..2088 /label=BBa_B0034 CDS 2095..2769 /label=mrfp1 misc_feature 2776..2800 /label=Help:Barcodes CDS 2095..2800 /label=BBa_E1010 stem_loop 2816..2835 /label=T7 TE misc_feature 2842..2842 /label=stop polyA_site 2836..2849 /label=polya misc_feature 2809..2849 /label=BBa_B0012 terminator 2809..2849 /label=BBa_B0012 stem_loop 2870..2892 /label=stem_loop misc_feature 2858..2903 /label=BBa_B0011 terminator 2858..2903 /label=BBa_B0011 terminator 2809..2903 /label=BBa_B0014 misc_feature 2077..2903 /label=BBa_K188634 ORIGIN 1 gctaaatgcg taaactgcat atgccttcgc tgagtgtaat ttacgttaca aattttaacg 61 aaacgggaac cctatattga tctctactta ctagatgcgt aaaggagaag aacttttcac 121 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt 181 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac 241 tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca 301 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc 361 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg 421 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga 481 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa 541 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca 601 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg 661 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa 721 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat 781 tacacatggc atggatgaac tatacaaata ataatactag agccatataa ggccatataa 841 ggccatataa ggccatataa ggccatataa ggccatataa ggccatataa ggccatataa 901 ggccatataa ggggatccaa gcttcatatg ttcccatcta taatcctccc tgattcttcg 961 ctgatatggt gctaaaaagt aaccaataaa tggtatttaa aatgcaaatt atcaggcgta 1021 ccctgaaacg tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg 1081 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg ccacaagttc 1141 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 1201 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc 1261 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 1321 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 1381 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 1441 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc 1501 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc 1561 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 1621 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 1681 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 1741 gggatcactc tcggcatgga cgagctgtac aagtaataat actagagcca ggcatcaaat 1801 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 1861 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 1921 agagatccta aaggggtatc ttaggaattt actttatttt tcatccccat cactcttgat 1981 cgttatcaat tcccacgctg tttcagagcg ttaccttgcc cttaaacatt agcaatgtcg 2041 atttatcaga gggccgacag gctcccacta ctagagaaag aggagaaata ctagatggct 2101 tcctccgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt 2161 aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag 2221 accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc 2281 ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac 2341 ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt 2401 ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt 2461 aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt 2521 tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa 2581 atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg 2641 gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc 2701 tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc 2761 accggtgctt aataacgctg atagtgctag tgtagatcgc tactagagtc acactggctc 2821 accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta 2881 ttaatccggc ttttttatta ttt //