##gff-version 3
##sequence-region BBa_K774102 1 2903
BBa_K774102	.	promoter	1925	2068	.	+	0	ID=BBa_M33001;Name=BBa_M33001
BBa_K774102	.	promoter	1925	2068	.	+	0	ID=annotation2069943;Name=narGp;Parent=BBa_M33001
BBa_K774102	.	CDS	95	814	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K774102	.	CDS	95	814	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K774102	.	engineered_region	823	1916	.	+	0	ID=BBa_K774006;Name=BBa_K774006
BBa_K774102	.	promoter	823	1030	.	+	0	ID=BBa_K774001;Name=BBa_K774001;Parent=BBa_K774006
BBa_K774102	.	sequence_feature	913	931	.	+	0	ID=annotation2177193;Name=BHN;Parent=BBa_K774001
BBa_K774102	.	promoter	823	912	.	+	0	ID=annotation2177192;Name=E9-ns2 CArG promoter;Parent=BBa_K774001
BBa_K774102	.	promoter	932	1030	.	+	0	ID=annotation2177194;Name=BBaK216005;Parent=BBa_K774001
BBa_K774102	.	engineered_region	1039	1916	.	+	0	ID=BBa_E0420;Name=BBa_E0420;Parent=BBa_K774006
BBa_K774102	.	ribosome_entry_site	1039	1050	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0420
BBa_K774102	.	conserved_region	1043	1046	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K774102	.	terminator	1788	1867	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0420
BBa_K774102	.	stem_loop	1799	1842	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K774102	.	engineered_region	1788	1867	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K774102	.	CDS	1057	1779	.	+	0	ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_E0420
BBa_K774102	.	terminator	1876	1916	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0420
BBa_K774102	.	stem_loop	1883	1902	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K774102	.	polyA_site	1903	1916	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K774102	.	stop_codon	1909	1909	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K774102	.	engineered_region	1876	1916	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K774102	.	CDS	2095	2775	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K774102	.	engineered_tag	2776	2800	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K774102	.	CDS	2095	2769	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K774102	.	promoter	1	88	.	+	0	ID=BBa_K125100;Name=BBa_K125100
BBa_K774102	.	promoter	1	88	.	+	0	ID=annotation1968486;Name=nir promoter;Parent=BBa_K125100
BBa_K774102	.	sequence_feature	73	78	.	+	0	ID=annotation1968487;Name=-10 sequence;Parent=BBa_K125100
BBa_K774102	.	non_covalent_binding_site	5	20	.	+	0	ID=annotation1968488;Name=Ntc-B binding motif;Parent=BBa_K125100
BBa_K774102	.	non_covalent_binding_site	36	44	.	+	0	ID=annotation1968489;Name=Ntc-A binding motif;Parent=BBa_K125100
BBa_K774102	.	engineered_region	2077	2903	.	+	0	ID=BBa_K188634;Name=BBa_K188634
BBa_K774102	.	CDS	2095	2800	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K188634
BBa_K774102	.	engineered_tag	2776	2800	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K774102	.	CDS	2095	2769	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K774102	.	terminator	2809	2903	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K188634
BBa_K774102	.	terminator	2858	2903	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K774102	.	engineered_region	2858	2903	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K774102	.	stem_loop	2870	2892	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K774102	.	terminator	2809	2849	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K774102	.	stem_loop	2816	2835	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K774102	.	polyA_site	2836	2849	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K774102	.	stop_codon	2842	2842	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K774102	.	engineered_region	2809	2849	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K774102	.	ribosome_entry_site	2077	2088	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K188634
BBa_K774102	.	conserved_region	2081	2084	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K774102
gctaaatgcgtaaactgcatatgccttcgctgagtgtaatttacgttacaaattttaacgaaacgggaaccctatattg
atctctacttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatg
ttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttg
cactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatac
ccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttca
aagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaagg
tattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatg
gcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcag
accattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgc
cctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggc
atggatgaactatacaaataataatactagagccatataaggccatataaggccatataaggccatataaggccatata
aggccatataaggccatataaggccatataaggccatataaggggatccaagcttcatatgttcccatctataatcctc
cctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaattatcaggcgtaccctgaa
acgtactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggt
cgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctg
accctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgc
agtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccagga
gcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaac
cgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagcc
acaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacgg
cagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactac
ctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccg
ccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttatatactagagatcctaaaggggtatcttaggaatttactttatttttcatccccatcactc
ttgatcgttatcaattcccacgctgtttcagagcgttaccttgcccttaaacattagcaatgtcgatttatcagagggc
cgacaggctcccactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgt
ttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaag
gtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttcca
gtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaa
tgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttca
tctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagc
ttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggt
cactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatca
aactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccgg
tgcttaataacgctgatagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttctgcgt
ttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt