BBa_K774102
type: ComponentDefinition
persistent identity: BBa K774102
display id: BBa_K774102
version: 1
was generated by: iGEM to SBOL conversion
description: Multi sensor - for calculation of specific concentrations of nitrates nitrites and nitric oxide usin
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
mutable provenance: all parts on registry
owned by: james
owned by: myers
mutable description: A serious problem encountered again and again by synthetic biologists is, that for a particular ligand specific promoters do not exist and that it is very difficult to construct a transcription factor that is specific to the required ligand. There are however often broad spectrum (non-specific) promoters for a ligand of interest. These promoters and their transcription factors will induce transcription initiation when exposed to (or not exposed to) the investigated ligand, but also when under influence of other similar ligands. Assuming competitive binding, this is an interesting effect which can be exploited to give specific and accurate concentrations of each of the inducing ligands. In its simplest form of the Multi-Sensor system, there are two different transcription factors, each of which will cause transcription when exposed to either one or both of two different competitive ligands. They both posses different active sites, which causes there to be a different bias in both of those for each ligand. This will lead to transcription rates of one of the promoters to indicate a ratio between the two different ligands (for example nitrates and nitrites) as seen in line 1 ("Figure 3."). Because the two different transcription factors have different binding efficiencies to the two ligands, the line of the other promoter will take a different angle (line 2). The point where these two lines cross, gives the precise concentration of both ligands even though the two different promoters are non-specific. Figure 4: Each plane represents a range of possible concentration ratios of three ligands at a particular transcription rate (arbitrary). The intersection of the two different planes is shown as a purple line. It represents the range of possible ratios when only two planes are observed. Figure 5: Each plane represents a range of possible concentration ratios of three ligands at a particular transcription rate (arbitrary). The intersection between each pair of planes is ind…
discontinued: true
dominant: false
experience: experience/None
group u list: _1026_
m user id: 0
owner id: 13139
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: there are possible issues with peak seperation in a flourimiter.
star: false
creator: Pascoe James Harvey
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_E1010
component: BBa_E0040
component: BBa_K125100
component: BBa_M33001
component: BBa_K774006
component: BBa_K188634
sequence annotation: BBa_K125100
sequence annotation: BBa_E0040
sequence annotation: BBa_K774006
sequence annotation: BBa_M33001
sequence annotation: BBa_K188634
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K774102_sequence/1

BBa_E1010
type: Component
persistent identity: component2202234
display id: component2202234
version: 1
top level: BBa_K774102
definition: mRFP1
access: public

BBa_E0040
type: Component
persistent identity: component2202214
display id: component2202214
version: 1
top level: BBa_K774102
definition: GFP
access: public

BBa_K125100
type: Component
persistent identity: component2202212
display id: component2202212
version: 1
top level: BBa_K774102
definition: BBa_K125100
access: public

BBa_M33001
type: Component
persistent identity: component2202231
display id: component2202231
version: 1
top level: BBa_K774102
definition: BBa_M33001
access: public

BBa_K774006
type: Component
persistent identity: component2202229
display id: component2202229
version: 1
top level: BBa_K774102
definition: MB-CFP
access: public

BBa_K188634
type: Component
persistent identity: component2202243
display id: component2202243
version: 1
top level: BBa_K774102
definition: BBa_K188634
access: public

BBa_K125100
type: SequenceAnnotation
persistent identity: annotation2202212
display id: annotation2202212
version: 1
top level: BBa_K774102
location: https://synbiohub.org/public/igem/BBa_K774102/annotation2202212/range2202212/1
component: BBa_K125100

https://synbiohub.org/public/igem/BBa_K774102/annotation2202212/range2202212/1
type: Range
persistent identity: range2202212
display id: range2202212
version: 1
top level: BBa_K774102
direction: direction/reverse
start: 1
end: 88
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2202214
display id: annotation2202214
version: 1
top level: BBa_K774102
location: https://synbiohub.org/public/igem/BBa_K774102/annotation2202214/range2202214/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K774102/annotation2202214/range2202214/1
type: Range
persistent identity: range2202214
display id: range2202214
version: 1
top level: BBa_K774102
direction: direction/reverse
start: 95
end: 814
orientation: inline

BBa_K774006
type: SequenceAnnotation
persistent identity: annotation2202229
display id: annotation2202229
version: 1
top level: BBa_K774102
location: https://synbiohub.org/public/igem/BBa_K774102/annotation2202229/range2202229/1
component: BBa_K774006

https://synbiohub.org/public/igem/BBa_K774102/annotation2202229/range2202229/1
type: Range
persistent identity: range2202229
display id: range2202229
version: 1
top level: BBa_K774102
direction: direction/reverse
start: 823
end: 1916
orientation: inline

BBa_M33001
type: SequenceAnnotation
persistent identity: annotation2202231
display id: annotation2202231
version: 1
top level: BBa_K774102
location: https://synbiohub.org/public/igem/BBa_K774102/annotation2202231/range2202231/1
component: BBa_M33001

https://synbiohub.org/public/igem/BBa_K774102/annotation2202231/range2202231/1
type: Range
persistent identity: range2202231
display id: range2202231
version: 1
top level: BBa_K774102
direction: direction/reverse
start: 1925
end: 2068
orientation: inline

BBa_K188634
type: SequenceAnnotation
persistent identity: annotation2202243
display id: annotation2202243
version: 1
top level: BBa_K774102
location: https://synbiohub.org/public/igem/BBa_K774102/annotation2202243/range2202243/1
component: BBa_K188634

https://synbiohub.org/public/igem/BBa_K774102/annotation2202243/range2202243/1
type: Range
persistent identity: range2202243
display id: range2202243
version: 1
top level: BBa_K774102
direction: direction/reverse
start: 2077
end: 2903
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2202234
display id: annotation2202234
version: 1
top level: BBa_K774102
location: https://synbiohub.org/public/igem/BBa_K774102/annotation2202234/range2202234/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K774102/annotation2202234/range2202234/1
type: Range
persistent identity: range2202234
display id: range2202234
version: 1
top level: BBa_K774102
direction: direction/reverse
start: 2095
end: 2775
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_K125100
type: ComponentDefinition
persistent identity: BBa K125100
display id: BBa_K125100
version: 1
was generated by: iGEM to SBOL conversion
description: nir promoter from Synechocystis sp. PCC6803
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: The ''nirA'' promoter originates from ''Synechocystis'' sp. PCC6803.
owned by: james
owned by: myers
mutable description: The ''nir'' promoter is nitrate inducible.
discontinued: false
dominant: false
experience: experience/None
group u list: _
m user id: 0
owner id: 3229
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Krystle Salazar and Grace Kwan
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: nir promoter
sequence annotation: Ntc-B binding motif
sequence annotation: Ntc-A binding motif
sequence annotation: -10 sequence
sequence: https://synbiohub.org/public/igem/BBa_K125100_sequence/1

nir promoter
type: SequenceAnnotation
persistent identity: annotation1968486
display id: annotation1968486
version: 1
top level: BBa_K125100
location: https://synbiohub.org/public/igem/BBa_K125100/annotation1968486/range1968486/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K125100/annotation1968486/range1968486/1
type: Range
persistent identity: range1968486
display id: range1968486
version: 1
top level: BBa_K125100
direction: direction/forward
start: 1
end: 88
orientation: inline

Ntc-B binding motif
type: SequenceAnnotation
persistent identity: annotation1968488
display id: annotation1968488
version: 1
top level: BBa_K125100
location: https://synbiohub.org/public/igem/BBa_K125100/annotation1968488/range1968488/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K125100/annotation1968488/range1968488/1
type: Range
persistent identity: range1968488
display id: range1968488
version: 1
top level: BBa_K125100
direction: direction/forward
start: 5
end: 20
orientation: inline

Ntc-A binding motif
type: SequenceAnnotation
persistent identity: annotation1968489
display id: annotation1968489
version: 1
top level: BBa_K125100
location: https://synbiohub.org/public/igem/BBa_K125100/annotation1968489/range1968489/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K125100/annotation1968489/range1968489/1
type: Range
persistent identity: range1968489
display id: range1968489
version: 1
top level: BBa_K125100
direction: direction/forward
start: 36
end: 44
orientation: inline

-10 sequence
type: SequenceAnnotation
persistent identity: annotation1968487
display id: annotation1968487
version: 1
top level: BBa_K125100
location: https://synbiohub.org/public/igem/BBa_K125100/annotation1968487/range1968487/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K125100/annotation1968487/range1968487/1
type: Range
persistent identity: range1968487
display id: range1968487
version: 1
top level: BBa_K125100
direction: direction/forward
start: 73
end: 78
orientation: inline

BBa_M33001
type: ComponentDefinition
persistent identity: BBa M33001
display id: BBa_M33001
version: 1
was generated by: iGEM to SBOL conversion
description: Nar operon promoter (narGp) => PoPs
created: 2010-05-31T11:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: Part was synthesized based on the promoter of the nar operon naturally present in E. coli.
owned by: james
owned by: myers
mutable description: The narG promoter is located at the beginning the nar operon and is naturally activated during nitrogen respiration of E. Coli by the Fnr protein to signal reduction of nitrate to nitrite. In the presence of nitrate, Fnr protein binds to a region upstream of narGp to act as an activator.
discontinued: false
dominant: false
experience: experience/None
group u list: _577_
m user id: 0
owner id: 6923
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Design includes BioBrick suffixes and prefixes, and the promoter sequence itself was checked for restriction digest sites to ensure compliance with Assembly Standard 10.
star: false
creator: Daniel Bui and Aaditya Shidham
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: narGp
sequence: https://synbiohub.org/public/igem/BBa_M33001_sequence/1

narGp
type: SequenceAnnotation
persistent identity: annotation2069943
display id: annotation2069943
version: 1
top level: BBa_M33001
location: https://synbiohub.org/public/igem/BBa_M33001/annotation2069943/range2069943/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_M33001/annotation2069943/range2069943/1
type: Range
persistent identity: range2069943
display id: range2069943
version: 1
top level: BBa_M33001
direction: direction/forward
start: 1
end: 144
orientation: inline

BBa_K188634
type: ComponentDefinition
persistent identity: BBa K188634
display id: BBa_K188634
version: 1
was generated by: iGEM to SBOL conversion
description: RBS+mRFP+double Terminator
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:13Z
mutable provenance: no
owned by: james
owned by: myers
mutable description: RBS + highly engineered mutant of red fluorescent protein from Discosoma striata (coral) + double terminator
discontinued: false
dominant: false
experience: experience/Works
group u list: _296_
m user id: 0
owner id: 4218
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no
star: false
creator: shau-yi wu
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_B0034
component: BBa_B0014
component: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K188634_sequence/1

BBa_B0034
type: Component
persistent identity: component2249810
display id: component2249810
version: 1
top level: BBa_K188634
definition: BBa_B0034
access: public

BBa_B0014
type: Component
persistent identity: component2249820
display id: component2249820
version: 1
top level: BBa_K188634
definition: BBa_B0014
access: public

BBa_E1010
type: Component
persistent identity: component2249813
display id: component2249813
version: 1
top level: BBa_K188634
definition: mRFP1
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2249810
display id: annotation2249810
version: 1
top level: BBa_K188634
location: https://synbiohub.org/public/igem/BBa_K188634/annotation2249810/range2249810/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K188634/annotation2249810/range2249810/1
type: Range
persistent identity: range2249810
display id: range2249810
version: 1
top level: BBa_K188634
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2249813
display id: annotation2249813
version: 1
top level: BBa_K188634
location: https://synbiohub.org/public/igem/BBa_K188634/annotation2249813/range2249813/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K188634/annotation2249813/range2249813/1
type: Range
persistent identity: range2249813
display id: range2249813
version: 1
top level: BBa_K188634
direction: direction/reverse
start: 19
end: 724
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2249820
display id: annotation2249820
version: 1
top level: BBa_K188634
location: https://synbiohub.org/public/igem/BBa_K188634/annotation2249820/range2249820/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K188634/annotation2249820/range2249820/1
type: Range
persistent identity: range2249820
display id: range2249820
version: 1
top level: BBa_K188634
direction: direction/reverse
start: 733
end: 827
orientation: inline

MB-CFP
type: ComponentDefinition
persistent identity: BBa K774006
display id: BBa_K774006
version: 1
was generated by: iGEM to SBOL conversion
description: Mammalian-Bacterial promoter with eCFP reporter: CArG promoter sequence + BBaK216005 + BBa_E0420
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:15Z
mutable provenance: E9-ns2 CArG promoter sequence + BHN + BBaK216005
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Our hybrid promoter hopes to add to the systems already in the registry by creating a hybrid promoter that combines the bacterial promoter PyeaR and the mammalian CArG element , in the orientation mammalian to bacterial, both of which respond to exogenous nitrogenous species. Combining the two would allow a more modular NO sensor that can be used in mammalian and bacterial cells interchangeably. The hybrid promoter has been attached to the reporter: enhanced Cyan Fluorescence Protein (eCFP). The hybrid promoter has been characterised by observing expression of flourescent protein, and found to have increased transcription in response to increasing concentrations of potassium nitrate.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1026_
m user id: 0
owner id: 12400
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: This biobrick contains the RBS and terminators, meaning we can simply add the promoter of choice to complete the system.
star: false
creator: NRP-UEA-Norwich
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_K774001
component: BBa_E0420
sequence annotation: BBa_K774001
sequence annotation: BBa_E0420
sequence: https://synbiohub.org/public/igem/BBa_K774006_sequence/1

BBa_K774001
type: Component
persistent identity: component2187180
display id: component2187180
version: 1
top level: MB-CFP
definition: M-B
access: public

BBa_E0420
type: Component
persistent identity: component2187190
display id: component2187190
version: 1
top level: MB-CFP
definition: BBa_E0420
access: public

BBa_K774001
type: SequenceAnnotation
persistent identity: annotation2187180
display id: annotation2187180
version: 1
top level: MB-CFP
location: https://synbiohub.org/public/igem/BBa_K774006/annotation2187180/range2187180/1
component: BBa_K774001

https://synbiohub.org/public/igem/BBa_K774006/annotation2187180/range2187180/1
type: Range
persistent identity: range2187180
display id: range2187180
version: 1
top level: MB-CFP
direction: direction/reverse
start: 1
end: 208
orientation: inline

BBa_E0420
type: SequenceAnnotation
persistent identity: annotation2187190
display id: annotation2187190
version: 1
top level: MB-CFP
location: https://synbiohub.org/public/igem/BBa_K774006/annotation2187190/range2187190/1
component: BBa_E0420

https://synbiohub.org/public/igem/BBa_K774006/annotation2187190/range2187190/1
type: Range
persistent identity: range2187190
display id: range2187190
version: 1
top level: MB-CFP
direction: direction/reverse
start: 217
end: 1094
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

M-B
type: ComponentDefinition
persistent identity: BBa K774001
display id: BBa_K774001
version: 1
was generated by: iGEM to SBOL conversion
description: Mammalian-Bacterial Promoter: E9-ns2 CArG promoter sequence + BHN + BBaK216005
created: 2012-06-24T11:00:00Z
modified: 2015-05-08T01:13:15Z
mutable provenance: PyeaR promoter (BBaK216005) and the E9-ns2 CArG promoter (Ref1) Ref1: Scott, S.D., Joiner, M.C. & Marples, B., 2002. Optimizing radiation-responsive gene promoters for radiogenetic cancer therapy. Gene therapy, 9(20), p.1396-402. Available at: http://www.ncbi.nlm.nih.gov/pubmed/12365005 [Accessed June 24, 2012].
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: A hybrid of the PyeaR promoter (BBaK216005) and the E9-ns2 CArG promoter (Ref1). Two versions will be synthesised, with the promoters altering their position in relation to the 5???-end of the sequence.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1026_
m user id: 0
owner id: 11706
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: To provide additional restriction enzyme sites that may become useful during later cloning steps, BamHI, HindIII and NdeI have been added between the 2 promoters. It is envisaged that any open reading frame (e.g. RFP or GFP) will be cloned ???downstream??? (i.e. at the 3???-end) of these promoter sequences.
star: false
creator: NRP-UEA-Norwich
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: E9-ns2 CArG promoter
sequence annotation: BHN
sequence annotation: BBaK216005
sequence: https://synbiohub.org/public/igem/BBa_K774001_sequence/1

E9-ns2 CArG promoter
type: SequenceAnnotation
persistent identity: annotation2177192
display id: annotation2177192
version: 1
top level: M-B
location: https://synbiohub.org/public/igem/BBa_K774001/annotation2177192/range2177192/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K774001/annotation2177192/range2177192/1
type: Range
persistent identity: range2177192
display id: range2177192
version: 1
top level: M-B
direction: direction/forward
start: 1
end: 90
orientation: inline

BHN
type: SequenceAnnotation
persistent identity: annotation2177193
display id: annotation2177193
version: 1
top level: M-B
location: https://synbiohub.org/public/igem/BBa_K774001/annotation2177193/range2177193/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K774001/annotation2177193/range2177193/1
type: Range
persistent identity: range2177193
display id: range2177193
version: 1
top level: M-B
direction: direction/forward
start: 91
end: 109
orientation: inline

BBaK216005
type: SequenceAnnotation
persistent identity: annotation2177194
display id: annotation2177194
version: 1
top level: M-B
location: https://synbiohub.org/public/igem/BBa_K774001/annotation2177194/range2177194/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K774001/annotation2177194/range2177194/1
type: Range
persistent identity: range2177194
display id: range2177194
version: 1
top level: M-B
direction: direction/forward
start: 110
end: 208
orientation: inline

BBa_E0420
type: ComponentDefinition
persistent identity: BBa E0420
display id: BBa_E0420
version: 1
was generated by: iGEM to SBOL conversion
description: ECFP (RBS+ LVA- TERM) (B0034.E0020.B0015)
created: 2004-03-15T12:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Standard CFP output device w/o LVA tag.
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_E0020
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0420_sequence/1

BBa_B0034
type: Component
persistent identity: component942780
display id: component942780
version: 1
top level: BBa_E0420
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component942797
display id: component942797
version: 1
top level: BBa_E0420
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component942787
display id: component942787
version: 1
top level: BBa_E0420
definition: BBa_B0010
access: public

BBa_E0020
type: Component
persistent identity: component942782
display id: component942782
version: 1
top level: BBa_E0420
definition: ecfp
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942780
display id: annotation942780
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942780/range942780/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0420/annotation942780/range942780/1
type: Range
persistent identity: range942780
display id: range942780
version: 1
top level: BBa_E0420
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation942782
display id: annotation942782
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942782/range942782/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_E0420/annotation942782/range942782/1
type: Range
persistent identity: range942782
display id: range942782
version: 1
top level: BBa_E0420
direction: direction/forward
start: 19
end: 741
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942787
display id: annotation942787
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942787/range942787/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0420/annotation942787/range942787/1
type: Range
persistent identity: range942787
display id: range942787
version: 1
top level: BBa_E0420
direction: direction/forward
start: 750
end: 829
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942797
display id: annotation942797
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942797/range942797/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0420/annotation942797/range942797/1
type: Range
persistent identity: range942797
display id: range942797
version: 1
top level: BBa_E0420
direction: direction/forward
start: 838
end: 878
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa E0020
display id: BBa_E0020
version: 1
was generated by: iGEM to SBOL conversion
description: engineered cyan fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_E0020_sequence/1

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K774102_sequence/1
type: Sequence
persistent identity: BBa K774102 sequence
display id: BBa_K774102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctaaatgcgtaaactgcatatgccttcgctgagtgtaatttacgttacaaattttaacgaaacgggaaccctatattgatctctacttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccatataaggccatataaggccatataaggccatataaggccatataaggccatataaggccatataaggccatataaggccatataaggggatccaagcttcatatgttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaattatcaggcgtaccctgaaacgtactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagatcctaaaggggtatcttaggaatttactttatttttcatccccatcactcttgatcgttatcaattcccacgctg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125100_sequence/1
type: Sequence
persistent identity: BBa K125100 sequence
display id: BBa_K125100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctaaatgcgtaaactgcatatgccttcgctgagtgtaatttacgttacaaattttaacgaaacgggaaccctatattgatctctact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M33001_sequence/1
type: Sequence
persistent identity: BBa M33001 sequence
display id: BBa_M33001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcctaaaggggtatcttaggaatttactttatttttcatccccatcactcttgatcgttatcaattcccacgctgtttcagagcgttaccttgcccttaaacattagcaatgtcgatttatcagagggccgacaggctcccac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K188634_sequence/1
type: Sequence
persistent identity: BBa K188634 sequence
display id: BBa_K188634_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K774006_sequence/1
type: Sequence
persistent identity: BBa K774006 sequence
display id: BBa_K774006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccatataaggccatataaggccatataaggccatataaggccatataaggccatataaggccatataaggccatataaggccatataaggggatccaagcttcatatgttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaattatcaggcgtaccctgaaacgtactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K774001_sequence/1
type: Sequence
persistent identity: BBa K774001 sequence
display id: BBa_K774001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccatataaggccatataaggccatataaggccatataaggccatataaggccatataaggccatataaggccatataaggccatataaggggatccaagcttcatatgttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaattatcaggcgtaccctgaaacg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0420_sequence/1
type: Sequence
persistent identity: BBa E0420 sequence
display id: BBa_E0420_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0020_sequence/1
type: Sequence
persistent identity: BBa E0020 sequence
display id: BBa_E0020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z