LOCUS BBa_K774103 3100 bp DNA linear 29-Nov-2024 DEFINITION Multi sensor - for calculation of specific concentrations of nitrates nitrites and nitric oxide ACCESSION BBa_K774103 VERSION BBa_K774103.1 FEATURES Location/Qualifiers promoter 1..88 /label=nir promoter misc_feature 73..78 /label=-10 sequence misc_feature 5..20 /label=Ntc-B binding motif misc_feature 36..44 /label=Ntc-A binding motif promoter 1..88 /label=BBa_K125100 RBS 97..109 /label=RBS-3\Weak RBS 103..106 /label=RBS misc_feature 97..109 /label=BBa_B0032 RBS 97..109 /label=BBa_B0032 misc_feature 116..118 /label=start misc_feature 869..871 /label=stop misc_feature 872..874 /label=stop misc_feature 832..868 /label=LVA tag CDS 119..831 /label=cds misc_feature 116..874 /label=BBa_K145015 stem_loop 894..937 /label=stem_loop misc_feature 883..962 /label=BBa_B0010 terminator 883..962 /label=BBa_B0010 stem_loop 978..997 /label=T7 TE misc_feature 1004..1004 /label=stop polyA_site 998..1011 /label=polya misc_feature 971..1011 /label=BBa_B0012 terminator 971..1011 /label=BBa_B0012 misc_feature 97..1011 /label=BBa_K175038 promoter 1020..1109 /label=E9-ns2 CArG promoter misc_feature 1110..1128 /label=BHN promoter 1129..1227 /label=BBaK216005 promoter 1020..1227 /label=BBa_K774001 misc_feature 1240..1243 /label=conserved RBS 1236..1247 /label=BBa_B0034 CDS 1254..1976 /label=BBa_E0020 stem_loop 1996..2039 /label=stem_loop misc_feature 1985..2064 /label=BBa_B0010 terminator 1985..2064 /label=BBa_B0010 stem_loop 2080..2099 /label=T7 TE misc_feature 2106..2106 /label=stop polyA_site 2100..2113 /label=polya misc_feature 2073..2113 /label=BBa_B0012 terminator 2073..2113 /label=BBa_B0012 misc_feature 1236..2113 /label=BBa_E0420 misc_feature 1020..2113 /label=BBa_K774006 promoter 2122..2265 /label=narGp promoter 2122..2265 /label=BBa_M33001 misc_feature 2278..2281 /label=conserved RBS 2274..2285 /label=BBa_B0034 CDS 2292..2966 /label=mrfp1 misc_feature 2973..2997 /label=Help:Barcodes CDS 2292..2997 /label=BBa_E1010 stem_loop 3013..3032 /label=T7 TE misc_feature 3039..3039 /label=stop polyA_site 3033..3046 /label=polya misc_feature 3006..3046 /label=BBa_B0012 terminator 3006..3046 /label=BBa_B0012 stem_loop 3067..3089 /label=stem_loop misc_feature 3055..3100 /label=BBa_B0011 terminator 3055..3100 /label=BBa_B0011 terminator 3006..3100 /label=BBa_B0014 misc_feature 2274..3100 /label=BBa_K188634 ORIGIN 1 gctaaatgcg taaactgcat atgccttcgc tgagtgtaat ttacgttaca aattttaacg 61 aaacgggaac cctatattga tctctactta ctagagtcac acaggaaagt actagatgcg 121 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt 181 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact 241 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac 301 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga 361 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga 421 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag 481 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga 541 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa 601 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta 661 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc 721 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga 781 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaaa ggcctgctgc 841 aaacgacgaa aactacgctt tagtagctta ataatactag agccaggcat caaataaaac 901 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 961 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagc 1021 catataaggc catataaggc catataaggc catataaggc catataaggc catataaggc 1081 catataaggc catataaggc catataaggg gatccaagct tcatatgttc ccatctataa 1141 tcctccctga ttcttcgctg atatggtgct aaaaagtaac caataaatgg tatttaaaat 1201 gcaaattatc aggcgtaccc tgaaacgtac tagagaaaga ggagaaatac tagatggtga 1261 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg 1321 tgaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc 1381 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga 1441 ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg 1501 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg 1561 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc 1621 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg 1681 agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca 1741 aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact 1801 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga 1861 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg 1921 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taataatact 1981 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 2041 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 2101 ttctgcgttt atatactaga gatcctaaag gggtatctta ggaatttact ttatttttca 2161 tccccatcac tcttgatcgt tatcaattcc cacgctgttt cagagcgtta ccttgccctt 2221 aaacattagc aatgtcgatt tatcagaggg ccgacaggct cccactacta gagaaagagg 2281 agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc 2341 gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc 2401 cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg 2461 cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg 2521 ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta 2581 tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg 2641 agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc 2701 agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc 2761 tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag 2821 ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca 2881 tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg 2941 ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac 3001 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagagagaa 3061 tataaaaagc cagattatta atccggcttt tttattattt //