##gff-version 3
##sequence-region BBa_K774103 1 3100
BBa_K774103	.	promoter	2122	2265	.	+	0	ID=BBa_M33001;Name=BBa_M33001
BBa_K774103	.	promoter	2122	2265	.	+	0	ID=annotation2069943;Name=narGp;Parent=BBa_M33001
BBa_K774103	.	engineered_region	2274	3100	.	+	0	ID=BBa_K188634;Name=BBa_K188634
BBa_K774103	.	terminator	3006	3100	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K188634
BBa_K774103	.	terminator	3006	3046	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K774103	.	polyA_site	3033	3046	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K774103	.	stop_codon	3039	3039	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K774103	.	stem_loop	3013	3032	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K774103	.	engineered_region	3006	3046	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K774103	.	terminator	3055	3100	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K774103	.	engineered_region	3055	3100	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K774103	.	stem_loop	3067	3089	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K774103	.	ribosome_entry_site	2274	2285	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K188634
BBa_K774103	.	conserved_region	2278	2281	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K774103	.	CDS	2292	2997	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K188634
BBa_K774103	.	CDS	2292	2966	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K774103	.	engineered_tag	2973	2997	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K774103	.	engineered_region	1020	2113	.	+	0	ID=BBa_K774006;Name=BBa_K774006
BBa_K774103	.	engineered_region	1236	2113	.	+	0	ID=BBa_E0420;Name=BBa_E0420;Parent=BBa_K774006
BBa_K774103	.	terminator	1985	2064	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0420
BBa_K774103	.	stem_loop	1996	2039	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K774103	.	engineered_region	1985	2064	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K774103	.	terminator	2073	2113	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0420
BBa_K774103	.	polyA_site	2100	2113	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K774103	.	stop_codon	2106	2106	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K774103	.	stem_loop	2080	2099	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K774103	.	engineered_region	2073	2113	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K774103	.	ribosome_entry_site	1236	1247	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0420
BBa_K774103	.	conserved_region	1240	1243	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K774103	.	CDS	1254	1976	.	+	0	ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_E0420
BBa_K774103	.	promoter	1020	1227	.	+	0	ID=BBa_K774001;Name=BBa_K774001;Parent=BBa_K774006
BBa_K774103	.	promoter	1129	1227	.	+	0	ID=annotation2177194;Name=BBaK216005;Parent=BBa_K774001
BBa_K774103	.	sequence_feature	1110	1128	.	+	0	ID=annotation2177193;Name=BHN;Parent=BBa_K774001
BBa_K774103	.	promoter	1020	1109	.	+	0	ID=annotation2177192;Name=E9-ns2 CArG promoter;Parent=BBa_K774001
BBa_K774103	.	promoter	1	88	.	+	0	ID=BBa_K125100;Name=BBa_K125100
BBa_K774103	.	non_covalent_binding_site	36	44	.	+	0	ID=annotation1968489;Name=Ntc-A binding motif;Parent=BBa_K125100
BBa_K774103	.	non_covalent_binding_site	5	20	.	+	0	ID=annotation1968488;Name=Ntc-B binding motif;Parent=BBa_K125100
BBa_K774103	.	sequence_feature	73	78	.	+	0	ID=annotation1968487;Name=-10 sequence;Parent=BBa_K125100
BBa_K774103	.	promoter	1	88	.	+	0	ID=annotation1968486;Name=nir promoter;Parent=BBa_K125100
BBa_K774103	.	engineered_region	97	1011	.	+	0	ID=BBa_K175038;Name=BBa_K175038
BBa_K774103	.	engineered_region	116	874	.	+	0	ID=BBa_K145015;Name=BBa_K145015;Parent=BBa_K175038
BBa_K774103	.	CDS	119	831	.	+	0	ID=annotation1969620;Name=cds;Parent=BBa_K145015
BBa_K774103	.	stop_codon	869	871	.	+	0	ID=annotation1969617;Name=stop;Parent=BBa_K145015
BBa_K774103	.	tag	832	868	.	+	0	ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015
BBa_K774103	.	stop_codon	872	874	.	+	0	ID=annotation1969618;Name=stop;Parent=BBa_K145015
BBa_K774103	.	start_codon	116	118	.	+	0	ID=annotation1969616;Name=start;Parent=BBa_K145015
BBa_K774103	.	terminator	883	962	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K175038
BBa_K774103	.	stem_loop	894	937	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K774103	.	engineered_region	883	962	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K774103	.	ribosome_entry_site	97	109	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K175038
BBa_K774103	.	ribosome_entry_site	97	109	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K774103	.	engineered_region	97	109	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K774103	.	ribosome_entry_site	103	106	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K774103	.	terminator	971	1011	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K175038
BBa_K774103	.	polyA_site	998	1011	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K774103	.	stop_codon	1004	1004	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K774103	.	stem_loop	978	997	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K774103	.	engineered_region	971	1011	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K774103
gctaaatgcgtaaactgcatatgccttcgctgagtgtaatttacgttacaaattttaacgaaacgggaaccctatattg
atctctacttactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattc
ttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaa
acttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggt
gttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtac
aggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgt
taatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataac
tcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaag
atggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaacca
ttacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaaca
gctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagctt
aataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccatataa
ggccatataaggccatataaggccatataaggccatataaggccatataaggccatataaggccatataaggccatata
aggggatccaagcttcatatgttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaata
aatggtatttaaaatgcaaattatcaggcgtaccctgaaacgtactagagaaagaggagaaatactagatggtgagcaa
gggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtg
tccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgc
cctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacga
cttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacc
cgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggca
acatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcat
caaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacaccccc
atcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgaga
agcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaata
atactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagatcctaaagggg
tatcttaggaatttactttatttttcatccccatcactcttgatcgttatcaattcccacgctgtttcagagcgttacc
ttgcccttaaacattagcaatgtcgatttatcagagggccgacaggctcccactactagagaaagaggagaaatactag
atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagt
tcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtcc
gctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatc
ccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtta
ccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccga
cggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaa
ggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaa
aaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgt
tgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaa
tccggcttttttattattt