LOCUS BBa_K775004 1166 bp DNA linear 22-Nov-2024 DEFINITION GPCR reporter FUS1-EGFP ACCESSION BBa_K775004 VERSION BBa_K775004.1 FEATURES Location/Qualifiers promoter 1..200 /label=FUS1 promoter misc_feature 201..206 /label=BamHi restriction site RBS 207..212 /label=Kozak sequence CDS 213..936 /label=EGFP misc_feature 937..942 /label=NdeI restriction site misc_feature 942..1166 /label=ADH1 terminator ORIGIN 1 ttttggtgcg atgatgaaac aaacatgaaa cgtcagtaat ttgaaacaaa taacgtaatt 61 ctcgggattg gttttattta aatgacaatg taagagtggc tttgtaaggt atgtgttgct 121 cttaaaatat ttggatacga catcctttat ctttttcctt taagagcagg atataagcca 181 tcaagtttct gaaaatcaaa ggatccaaaa aaatggtgag caagggcgag gagctgttca 241 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 301 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 361 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc 421 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 481 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 541 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 601 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 661 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 721 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 781 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 841 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 901 tcactctcgg catggacgag ctgtacaagt aataacatat gtaataagcg aatttcttat 961 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa 1021 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca 1081 ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc 1141 cgagcaaatg cctgcaaatc gctccc //