LOCUS       BBa_K775005             2588 bp    DNA     linear       01-Jan-2026
DEFINITION  Niacin Chimeric GPCR with Fus1-EGFP reporter
ACCESSION   BBa_K775005
VERSION     BBa_K775005.1  
FEATURES             Location/Qualifiers
     promoter        1..112
                     /label=GPD promoter
     misc_feature    113..118
                     /label=BamHI restriction site
     RBS             119..124
                     /label=Kozak sequence
     misc_feature    125..127
                     /label=Startcodon
     misc_feature    128..151
                     /label=FLAGtag
     misc_feature    152..337
                     /label=N terminus RI7
     misc_feature    1151..1156
                     /label=Stopcodon
     misc_feature    1157..1162
                     /label=NdeI restriction site
     misc_feature    1163..1414
                     /label=CYC1 terminator
     misc_feature    1055..1150
                     /label=C terminus RI7
     CDS             338..1054
                     /label=Niacin receptor part
     CDS             1..1414
                     /label=BBa_K775000
     promoter        1423..1622
                     /label=FUS1 promoter
     misc_feature    1623..1628
                     /label=BamHi restriction site
     RBS             1629..1634
                     /label=Kozak sequence
     CDS             1635..2358
                     /label=EGFP
     misc_feature    2359..2364
                     /label=NdeI restriction site
     misc_feature    2364..2588
                     /label=ADH1 terminator
     misc_feature    1423..2588
                     /label=BBa_K775004
ORIGIN
        1 cggtaggtat tgattgtaat tctgtaaatc tatttcttaa acttcttaaa ttctactttt
       61 atagttagtc ttttttttag ttttaaaaca ccagaactta gtttcgacgg atggatccaa
      121 aaaaatggac tacaaggacg acgacgacaa ggagcgaagg aaccacagtg ggagagtgag
      181 tgaatttgtg ttgctgggtt tcccagctcc tgccccactg cgagtactac tatttttcct
      241 ttctcttctg gcctatgtgt tggtgttgac tgaaaacatg ctcatcatta tagcaattag
      301 gaaccaccca accctccaca aacccatgta tcatctcaaa tcctggaaat ccagccggat
      361 tttcttgttc aacctggccg tggctgactt tctcctgatc atttgcttgc cgttcttgac
      421 ggacaactat gtccagaact gggactggag gttcgggagc atcccctgcc gcgtgatgct
      481 cttcatgttg gccatgaacc gacagggcag catcatcttc ctcacggtgg tggctgtgga
      541 caggtacttc agggtggtcc acccgcacca cttcctgaac aagatctcca accggacggc
      601 ggccatcatc tcttgcttcc tgtggggcat caccatcggc ctgacagtcc acctcctcta
      661 cacggacatg atgacccgaa acggcgatgc aaacctgtgc agcagtttta gcatctgcta
      721 cactttcagg tggcacgatg caatgttcct cttggagttc ttcctgcccc tgggcatcat
      781 cctgttctgc tctggcagga tcatttggag cctaaggcag agacagatgg acaggcacgt
      841 caagatcaag agggccatca acttcatcat ggtggttgcc attgtgtttg tcatctgctt
      901 cctgcccagt gtggccgtga ggacccgcat cttctggctc ctctacaaac acaacgtgag
      961 gaactgtgac atctactcct ctgtggactt ggccttcttc accaccctta gctttaccta
     1021 catgaacagc atgctcgacc cggtggtcta ctactgcttg cgcaaccaag atgtcaaaag
     1081 agcgctacgt cgcacgctgc acctggccca ggaccaggag gccaatacca acaaaggcag
     1141 caaaattggt taataacata tgtcatgtaa ttagttatgt cacgcttaca ttcacgccct
     1201 ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct
     1261 atttattttt ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt
     1321 ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag
     1381 gttttgggac gctcgaaggc tttaatttgc ggcctactag agttttggtg cgatgatgaa
     1441 acaaacatga aacgtcagta atttgaaaca aataacgtaa ttctcgggat tggttttatt
     1501 taaatgacaa tgtaagagtg gctttgtaag gtatgtgttg ctcttaaaat atttggatac
     1561 gacatccttt atctttttcc tttaagagca ggatataagc catcaagttt ctgaaaatca
     1621 aaggatccaa aaaaatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     1681 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1741 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1801 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     1861 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1921 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1981 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     2041 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     2101 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     2161 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     2221 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     2281 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     2341 agctgtacaa gtaataacat atgtaataag cgaatttctt atgatttatg atttttatta
     2401 ttaaataagt tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa
     2461 aacgaaaatt cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat
     2521 agcatgaggt cgctcttatt gaccacacct ctaccggcat gccgagcaaa tgcctgcaaa
     2581 tcgctccc
//