LOCUS       BBa_K775006             2597 bp    DNA     linear       17-May-2024
DEFINITION  Isoamylacetate Chimeric GPCR with Fus1-EGFP reporter
ACCESSION   BBa_K775006
VERSION     BBa_K775006.1  
FEATURES             Location/Qualifiers
     CDS             1..1423
                     /label=BBa_K775001
     promoter        1432..1631
                     /label=FUS1 promoter
     misc_feature    1632..1637
                     /label=BamHi restriction site
     RBS             1638..1643
                     /label=Kozak sequence
     CDS             1644..2367
                     /label=EGFP
     misc_feature    2368..2373
                     /label=NdeI restriction site
     misc_feature    2373..2597
                     /label=ADH1 terminator
     misc_feature    1432..2597
                     /label=BBa_K775004
ORIGIN
        1 cggtaggtat tgattgtaat tctgtaaatc tatttcttaa acttcttaaa ttctactttt
       61 atagttagtc ttttttttag ttttaaaaca ccagaactta gtttcgacgg atggatccaa
      121 aaaaatggac tacaaggacg acgacgacaa ggagcgaagg aaccacagtg ggagagtgag
      181 tgaatttgtg ttgctgggtt tcccagctcc tgccccactg cgagtactac tatttttcct
      241 ttctcttctg gcctatgtgt tggtgttgac tgaaaacatg ctcatcatta tagcaattag
      301 gaaccaccca accctccaca aacccatgta tcacctggac actcgactcc acatccccat
      361 gtactttttt ctcagccact tgtcctttgt ggatgcctgc tcctcttctg tcataagtcc
      421 taagatgttg tctgacatgt ttgtggataa aaaggtgatc tccttcttag gttgtgctat
      481 ccagctttgt ttatttagcc agtttgtagt gacagaatgt ttcctcctgg cctccatggc
      541 ttatgatcgg tatgttgcca tctgtaagcc tttgctgtat actctcatca tgtcccagcg
      601 agtctgtgta cagttagtga ttggccctta cagcataggc tttgtaagca ctatggtcca
      661 tattatttct gcatttgtcc ttccatactg tggtccaaat ctcatcaatc actttttctg
      721 tgaccttctt cctgtcctct cactggcatg tgcaaatact cagatgaaaa aacgtttgct
      781 tttcatcgtg gctgggattc taggtgtatt cagtggtata atcatattgg tgtcctatgt
      841 ttacattgcc atcaccatcc taaagatcag ttctgctgat gggaggcgca aagccttctc
      901 cacctgctct tcacacctga cagcagtctc tattctttac gggactctct tctttatcta
      961 tgtacgtcca agctccagct tctctctgga tatcaataag gttgtgtcac tattttatac
     1021 cactgttatc cctatgttga acccattcat ttatagcctg tactgcttgc gcaaccaaga
     1081 tgtcaaaaga gcgctacgtc gcacgctgca cctggcccag gaccaggagg ccaataccaa
     1141 caaaggcagc aaaattggtt aataacatat gtcatgtaat tagttatgtc acgcttacat
     1201 tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc
     1261 taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa
     1321 atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg
     1381 cttgagaagg ttttgggacg ctcgaaggct ttaatttgcg gcctactaga gttttggtgc
     1441 gatgatgaaa caaacatgaa acgtcagtaa tttgaaacaa ataacgtaat tctcgggatt
     1501 ggttttattt aaatgacaat gtaagagtgg ctttgtaagg tatgtgttgc tcttaaaata
     1561 tttggatacg acatccttta tctttttcct ttaagagcag gatataagcc atcaagtttc
     1621 tgaaaatcaa aggatccaaa aaaatggtga gcaagggcga ggagctgttc accggggtgg
     1681 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1741 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     1801 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     1861 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1921 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1981 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2041 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     2101 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     2161 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2221 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     2281 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2341 gcatggacga gctgtacaag taataacata tgtaataagc gaatttctta tgatttatga
     2401 tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt
     2461 aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt
     2521 ctcaggtata gcatgaggtc gctcttattg accacacctc taccggcatg ccgagcaaat
     2581 gcctgcaaat cgctccc
//