LOCUS       BBa_K775007             2594 bp    DNA     linear       22-Nov-2024
DEFINITION  OR1G1 Isoamylacetate Chimeric GPCR  with Fus1-EGFP reporter
ACCESSION   BBa_K775007
VERSION     BBa_K775007.1  
FEATURES             Location/Qualifiers
     CDS             1..1420
                     /label=BBa_K775002
     promoter        1429..1628
                     /label=FUS1 promoter
     misc_feature    1629..1634
                     /label=BamHi restriction site
     RBS             1635..1640
                     /label=Kozak sequence
     CDS             1641..2364
                     /label=EGFP
     misc_feature    2365..2370
                     /label=NdeI restriction site
     misc_feature    2370..2594
                     /label=ADH1 terminator
     misc_feature    1429..2594
                     /label=BBa_K775004
ORIGIN
        1 cggtaggtat tgattgtaat tctgtaaatc tatttcttaa acttcttaaa ttctactttt
       61 atagttagtc ttttttttag ttttaaaaca ccagaactta gtttcgacgg atggatccaa
      121 aaaaatggac tacaaggacg acgacgacaa ggagcgaagg aaccacagtg ggagagtgag
      181 tgaatttgtg ttgctgggtt tcccagctcc tgccccactg cgagtactac tatttttcct
      241 ttctcttctg gcctatgtgt tggtgttgac tgaaaacatg ctcatcatta tagcaattag
      301 gaaccaccca accctccaca aacccatgta tactgacact caactccata cccccatgta
      361 cttctttcta gccaacctct cccttgcaga tgcctgcttt gtgtccacca cagtccctaa
      421 gatgctggca aacatacaga tccagagtca ggccatctcc tactcagggt gtctactaca
      481 gttgtatttt ttcatgttat ttgtgatgct ggaggcattc ctcttggcgg tcatggccta
      541 tgactgctac gtggccattt gccacccact tcattacatt ctgatcatga gccctgggct
      601 ctgcatcttc ctcgtgtctg catcctggat catgaatgcc ctccactccc ttctacacac
      661 acttctgatg aacagcctgt ccttctgcgc aaaccatgag atcccacact tcttctgtga
      721 catcaatccc ctcctgagtc tgtcctgcac agaccccttc accaatgagc tggtgatctt
      781 catcactggg ggtctcacag gactcatttg tgtgctttgc ctgattatct cttacacgaa
      841 cgttttctcg accatcctga agatcccatc agctcagggg aagcggaaag ccttttccac
      901 ctgtagctct catctctccg tggtctctct cttctttggg acttcttttt gtgttgattt
      961 cagttctccc tcaacccact cggcccagaa ggacacagtt gcatcagtga tgtacacagt
     1021 ggtaactcca atgttgaatc cctttatcta cagtttgtac tgcttgcgca accaagatgt
     1081 caaaagagcg ctacgtcgca cgctgcacct ggcccaggac caggaggcca ataccaacaa
     1141 aggcagcaaa attggttaat aacatatgtc atgtaattag ttatgtcacg cttacattca
     1201 cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag
     1261 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt
     1321 tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt
     1381 gagaaggttt tgggacgctc gaaggcttta atttgcggcc tactagagtt ttggtgcgat
     1441 gatgaaacaa acatgaaacg tcagtaattt gaaacaaata acgtaattct cgggattggt
     1501 tttatttaaa tgacaatgta agagtggctt tgtaaggtat gtgttgctct taaaatattt
     1561 ggatacgaca tcctttatct ttttccttta agagcaggat ataagccatc aagtttctga
     1621 aaatcaaagg atccaaaaaa atggtgagca agggcgagga gctgttcacc ggggtggtgc
     1681 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1741 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     1801 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     1861 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     1921 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1981 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2041 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     2101 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     2161 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2221 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     2281 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     2341 tggacgagct gtacaagtaa taacatatgt aataagcgaa tttcttatga tttatgattt
     2401 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
     2461 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
     2521 aggtatagca tgaggtcgct cttattgacc acacctctac cggcatgccg agcaaatgcc
     2581 tgcaaatcgc tccc
//