BBa_K775003
1
BBa_K775003
RI7-odr10 (diacetyl) chimeric GPCR with promoter and terminator
2012-09-04T11:00:00Z
2015-05-08T01:13:16Z
N-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat)
Ligand binding domain: C. elegans 7-transmembrane receptor ODR-10 U49449.1
C-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat)
Released HQ 2013
BBa_K775003 encodes a chimeric diacetyl-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the C. elegans 7-transmembrane receptor ODR-10, flanked by the N- and C- terminals of the rat OR RI7.
false
false
_1027_
0
12035
9
In stock
false
-
false
Sietske Grijseels
BBa_K775004
1
BBa_K775004
GPCR reporter FUS1-EGFP
2012-09-20T11:00:00Z
2015-05-08T01:13:16Z
The sequence of the Fus1 promoter comes from S. cerevisiae
This reporter can be used in combination with the expression of a G-protein coupled receptor in yeast. The Fus1 promoter is a promoter in the mating pathway (a G-protein coupled receptor pathway. If the receptor of this pathway is activated by the pheromone of the opposite mating type the mating pathway is activated and the Fus1 promoter is switched on.
false
false
_1027_
0
12035
9
It's complicated
true
-
false
Sietske Grijseels
annotation2191553
1
EGFP
range2191553
1
213
936
annotation2191552
1
Kozak sequence
range2191552
1
207
212
annotation2191550
1
FUS1 promoter
range2191550
1
1
200
annotation2191551
1
BamHi restriction site
range2191551
1
201
206
annotation2191555
1
ADH1 terminator
range2191555
1
942
1166
annotation2191554
1
NdeI restriction site
range2191554
1
937
942
BBa_K775008
1
BBa_K775008
diacetyl chimeric GPCR with Fus1-EGFP reporter
2012-09-21T11:00:00Z
2015-05-08T01:13:16Z
-
This part is a combination between BBa_K775003(diacetyl chimeric GPCR)and BBa_K775004 (Fus1-EGFP repoter).
false
true
_1027_
0
12035
9
It's complicated
false
-
false
Sietske Grijseels
component2203271
1
BBa_K775004
component2203264
1
BBa_K775003
annotation2203271
1
BBa_K775004
range2203271
1
1453
2618
annotation2203264
1
BBa_K775003
range2203264
1
1
1444
BBa_K775004_sequence
1
ttttggtgcgatgatgaaacaaacatgaaacgtcagtaatttgaaacaaataacgtaattctcgggattggttttatttaaatgacaatgtaagagtggctttgtaaggtatgtgttgctcttaaaatatttggatacgacatcctttatctttttcctttaagagcaggatataagccatcaagtttctgaaaatcaaaggatccaaaaaaatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataacatatgtaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc
BBa_K775003_sequence
1
cggtaggtattgattgtaattctgtaaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagttttaaaacaccagaacttagtttcgacggatggatccaaaaaaatggagcgaaggaaccacagtgggagagtgagtgaatttgtgttgctgggtttcccagctcctgccccactgcgagtactactatttttcctttctcttctggcctatgtgttggtgttgactgaaaacatgctcatcattatagcaattaggaaccacccaaccctccacaaacccatgtattttttctcagtattctcgattttttacgccatcatcgagttcatattacgacctataatgcatattgagaacaccactttctttttgatctcaaggaaaagattcaactactccaccaaacttggaaaaatcaactctgcgttttactgtgcttgttttgccaccagttttgttgtctcaggagttcactttgtttatcgatattttgcaacttgcaaaccgaatctacttcgtttgttcaacttgccaactcttctactttggccacttggttgcagtgtacccgtgacaatgtgggctagtgtctcatattttttgtatccagataccgagtacacggaagcggctgtcaccaatgtactaaataaccactataactggatcaaaaaggagaatgtatcgtacattgcatacgtctattaccaatacgaaaacggagtaaggcatatctacctcaaaaacttgcttggatgctttgttcattactttgtcatgtcgatgacgtttgttgtgatgttctactgcggatatgccacgtggaaaactatgaatgaacacaaggatgtatctgatagaactcgagcgctacagaaacaacttttcaaagctttagttcttcagacactcatcccaactatcttcatgtacgccccaactggagtcatgttcatcgcaccgttttttgacgtgaatttgaatgcaaacgccaatttcattgtgttttgctcatttctgtacccgggactcgatccactcattctgattttgatcatttactgcttgcgcaaccaagatgtcaaaagagcgctacgtcgcacgctgcacctggcccaggaccaggaggccaataccaacaaaggcagcaaaattggttaataacatatgtcatgtaattagttatgtcacgcttacattcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgcggcc
BBa_K775008_sequence
1
cggtaggtattgattgtaattctgtaaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagttttaaaacaccagaacttagtttcgacggatggatccaaaaaaatggagcgaaggaaccacagtgggagagtgagtgaatttgtgttgctgggtttcccagctcctgccccactgcgagtactactatttttcctttctcttctggcctatgtgttggtgttgactgaaaacatgctcatcattatagcaattaggaaccacccaaccctccacaaacccatgtattttttctcagtattctcgattttttacgccatcatcgagttcatattacgacctataatgcatattgagaacaccactttctttttgatctcaaggaaaagattcaactactccaccaaacttggaaaaatcaactctgcgttttactgtgcttgttttgccaccagttttgttgtctcaggagttcactttgtttatcgatattttgcaacttgcaaaccgaatctacttcgtttgttcaacttgccaactcttctactttggccacttggttgcagtgtacccgtgacaatgtgggctagtgtctcatattttttgtatccagataccgagtacacggaagcggctgtcaccaatgtactaaataaccactataactggatcaaaaaggagaatgtatcgtacattgcatacgtctattaccaatacgaaaacggagtaaggcatatctacctcaaaaacttgcttggatgctttgttcattactttgtcatgtcgatgacgtttgttgtgatgttctactgcggatatgccacgtggaaaactatgaatgaacacaaggatgtatctgatagaactcgagcgctacagaaacaacttttcaaagctttagttcttcagacactcatcccaactatcttcatgtacgccccaactggagtcatgttcatcgcaccgttttttgacgtgaatttgaatgcaaacgccaatttcattgtgttttgctcatttctgtacccgggactcgatccactcattctgattttgatcatttactgcttgcgcaaccaagatgtcaaaagagcgctacgtcgcacgctgcacctggcccaggaccaggaggccaataccaacaaaggcagcaaaattggttaataacatatgtcatgtaattagttatgtcacgcttacattcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgcggcctactagagttttggtgcgatgatgaaacaaacatgaaacgtcagtaatttgaaacaaataacgtaattctcgggattggttttatttaaatgacaatgtaagagtggctttgtaaggtatgtgttgctcttaaaatatttggatacgacatcctttatctttttcctttaagagcaggatataagccatcaagtttctgaaaatcaaaggatccaaaaaaatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataacatatgtaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z