BBa_K775009 1 BBa_K775009 Niacin Chimeric GPCR with Fus1-EGFP reporter (2) 2012-09-23T11:00:00Z 2015-05-08T01:13:16Z - Released HQ 2013 - false false _1027_ 0 12035 9 In stock false - false Sietske Grijseels component2195587 1 BBa_K775000 component2195575 1 BBa_K775004 annotation2195575 1 BBa_K775004 range2195575 1 1 1166 annotation2195587 1 BBa_K775000 range2195587 1 1175 2588 BBa_K775004 1 BBa_K775004 GPCR reporter FUS1-EGFP 2012-09-20T11:00:00Z 2015-05-08T01:13:16Z The sequence of the Fus1 promoter comes from S. cerevisiae This reporter can be used in combination with the expression of a G-protein coupled receptor in yeast. The Fus1 promoter is a promoter in the mating pathway (a G-protein coupled receptor pathway. If the receptor of this pathway is activated by the pheromone of the opposite mating type the mating pathway is activated and the Fus1 promoter is switched on. false false _1027_ 0 12035 9 It's complicated true - false Sietske Grijseels annotation2191552 1 Kozak sequence range2191552 1 207 212 annotation2191553 1 EGFP range2191553 1 213 936 annotation2191555 1 ADH1 terminator range2191555 1 942 1166 annotation2191554 1 NdeI restriction site range2191554 1 937 942 annotation2191550 1 FUS1 promoter range2191550 1 1 200 annotation2191551 1 BamHi restriction site range2191551 1 201 206 BBa_K775000 1 BBa_K775000 RI7-GPR109A Niacin chimeric GPCR 2012-08-30T11:00:00Z 2015-05-08T01:13:15Z Homo sapiens NP_808219.1 Rat Released HQ 2013 BBa_K775000 encodes a chimeric niacin-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the mammalian GPR109A, flanked by the N- and C- terminals of the rat OR RI7. false false _1027_ 0 12035 9 In stock false Promoter: GPD Terminator: CYC1. false Sietske Grijseels annotation2181640 1 Kozak sequence range2181640 1 119 124 annotation2181642 1 FLAGtag range2181642 1 128 151 annotation2181646 1 CYC1 terminator range2181646 1 1163 1414 annotation2181648 1 Niacin receptor part range2181648 1 338 1054 annotation2181643 1 N terminus RI7 range2181643 1 152 337 annotation2181647 1 C terminus RI7 range2181647 1 1055 1150 annotation2181639 1 BamHI restriction site range2181639 1 113 118 annotation2181638 1 GPD promoter range2181638 1 1 112 annotation2181645 1 NdeI restriction site range2181645 1 1157 1162 annotation2181644 1 Stopcodon range2181644 1 1151 1156 annotation2181641 1 Startcodon range2181641 1 125 127 BBa_K775004_sequence 1 ttttggtgcgatgatgaaacaaacatgaaacgtcagtaatttgaaacaaataacgtaattctcgggattggttttatttaaatgacaatgtaagagtggctttgtaaggtatgtgttgctcttaaaatatttggatacgacatcctttatctttttcctttaagagcaggatataagccatcaagtttctgaaaatcaaaggatccaaaaaaatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataacatatgtaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc BBa_K775009_sequence 1 ttttggtgcgatgatgaaacaaacatgaaacgtcagtaatttgaaacaaataacgtaattctcgggattggttttatttaaatgacaatgtaagagtggctttgtaaggtatgtgttgctcttaaaatatttggatacgacatcctttatctttttcctttaagagcaggatataagccatcaagtttctgaaaatcaaaggatccaaaaaaatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataacatatgtaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccctactagagcggtaggtattgattgtaattctgtaaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagttttaaaacaccagaacttagtttcgacggatggatccaaaaaaatggactacaaggacgacgacgacaaggagcgaaggaaccacagtgggagagtgagtgaatttgtgttgctgggtttcccagctcctgccccactgcgagtactactatttttcctttctcttctggcctatgtgttggtgttgactgaaaacatgctcatcattatagcaattaggaaccacccaaccctccacaaacccatgtatcatctcaaatcctggaaatccagccggattttcttgttcaacctggccgtggctgactttctcctgatcatttgcttgccgttcttgacggacaactatgtccagaactgggactggaggttcgggagcatcccctgccgcgtgatgctcttcatgttggccatgaaccgacagggcagcatcatcttcctcacggtggtggctgtggacaggtacttcagggtggtccacccgcaccacttcctgaacaagatctccaaccggacggcggccatcatctcttgcttcctgtggggcatcaccatcggcctgacagtccacctcctctacacggacatgatgacccgaaacggcgatgcaaacctgtgcagcagttttagcatctgctacactttcaggtggcacgatgcaatgttcctcttggagttcttcctgcccctgggcatcatcctgttctgctctggcaggatcatttggagcctaaggcagagacagatggacaggcacgtcaagatcaagagggccatcaacttcatcatggtggttgccattgtgtttgtcatctgcttcctgcccagtgtggccgtgaggacccgcatcttctggctcctctacaaacacaacgtgaggaactgtgacatctactcctctgtggacttggccttcttcaccacccttagctttacctacatgaacagcatgctcgacccggtggtctactactgcttgcgcaaccaagatgtcaaaagagcgctacgtcgcacgctgcacctggcccaggaccaggaggccaataccaacaaaggcagcaaaattggttaataacatatgtcatgtaattagttatgtcacgcttacattcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgcggcc BBa_K775000_sequence 1 cggtaggtattgattgtaattctgtaaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagttttaaaacaccagaacttagtttcgacggatggatccaaaaaaatggactacaaggacgacgacgacaaggagcgaaggaaccacagtgggagagtgagtgaatttgtgttgctgggtttcccagctcctgccccactgcgagtactactatttttcctttctcttctggcctatgtgttggtgttgactgaaaacatgctcatcattatagcaattaggaaccacccaaccctccacaaacccatgtatcatctcaaatcctggaaatccagccggattttcttgttcaacctggccgtggctgactttctcctgatcatttgcttgccgttcttgacggacaactatgtccagaactgggactggaggttcgggagcatcccctgccgcgtgatgctcttcatgttggccatgaaccgacagggcagcatcatcttcctcacggtggtggctgtggacaggtacttcagggtggtccacccgcaccacttcctgaacaagatctccaaccggacggcggccatcatctcttgcttcctgtggggcatcaccatcggcctgacagtccacctcctctacacggacatgatgacccgaaacggcgatgcaaacctgtgcagcagttttagcatctgctacactttcaggtggcacgatgcaatgttcctcttggagttcttcctgcccctgggcatcatcctgttctgctctggcaggatcatttggagcctaaggcagagacagatggacaggcacgtcaagatcaagagggccatcaacttcatcatggtggttgccattgtgtttgtcatctgcttcctgcccagtgtggccgtgaggacccgcatcttctggctcctctacaaacacaacgtgaggaactgtgacatctactcctctgtggacttggccttcttcaccacccttagctttacctacatgaacagcatgctcgacccggtggtctactactgcttgcgcaaccaagatgtcaaaagagcgctacgtcgcacgctgcacctggcccaggaccaggaggccaataccaacaaaggcagcaaaattggttaataacatatgtcatgtaattagttatgtcacgcttacattcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgcggcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z